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Relative contributions of adipose-resident CD146(+) pericytes and CD34(+) adventitial progenitor cells in bone tissue engineering
Pericytes and other perivascular stem/stromal cells are of growing interest in the field of tissue engineering. A portion of perivascular cells are well recognized to have MSC (mesenchymal stem cell) characteristics, including multipotentiality, self-renewal, immunoregulatory functions, and diverse...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323123/ https://www.ncbi.nlm.nih.gov/pubmed/30622740 http://dx.doi.org/10.1038/s41536-018-0063-2 |
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author | Wang, Yiyun Xu, Jiajia Chang, Leslie Meyers, Carolyn A. Zhang, Lei Broderick, Kristen Lee, Min Peault, Bruno James, Aaron W. |
author_facet | Wang, Yiyun Xu, Jiajia Chang, Leslie Meyers, Carolyn A. Zhang, Lei Broderick, Kristen Lee, Min Peault, Bruno James, Aaron W. |
author_sort | Wang, Yiyun |
collection | PubMed |
description | Pericytes and other perivascular stem/stromal cells are of growing interest in the field of tissue engineering. A portion of perivascular cells are well recognized to have MSC (mesenchymal stem cell) characteristics, including multipotentiality, self-renewal, immunoregulatory functions, and diverse roles in tissue repair. Here, we investigate the differential but overlapping roles of two perivascular cell subsets in paracrine induction of bone repair. CD146(+)CD34(−)CD31(−)CD45(−)pericytes and CD34(+)CD146(−)CD31(−)CD45(−)adventitial cells were derived from human adipose tissue and applied alone or in combination to calvarial bone defects in mice. In vitro, osteogenic differentiation and tubulogenesis assays were performed using either fluorescence activated cell sorting-derived CD146(+) pericytes or CD34(+) adventitial cells. Results showed that CD146(+) pericytes induced increased cord formation in vitro and angiogenesis in vivo in comparison with patient-matched CD34(+) adventitial cells. In contrast, CD34(+) adventitial cells demonstrated heightened paracrine-induced osteogenesis in vitro. When applied in a critical-size calvarial defect model in NOD/SCID mice, the combination treatment of CD146(+) pericytes with CD34(+) adventitial cells led to greater re-ossification than either cell type alone. In summary, adipose-derived CD146(+) pericytes and CD34(+) adventitial cells display functionally distinct yet overlapping and complementary roles in bone defect repair. Consequently, CD146(+) pericytes and CD34(+) adventitial cells may demonstrate synergistic bone healing when applied as a combination cellular therapy. |
format | Online Article Text |
id | pubmed-6323123 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-63231232019-01-08 Relative contributions of adipose-resident CD146(+) pericytes and CD34(+) adventitial progenitor cells in bone tissue engineering Wang, Yiyun Xu, Jiajia Chang, Leslie Meyers, Carolyn A. Zhang, Lei Broderick, Kristen Lee, Min Peault, Bruno James, Aaron W. NPJ Regen Med Article Pericytes and other perivascular stem/stromal cells are of growing interest in the field of tissue engineering. A portion of perivascular cells are well recognized to have MSC (mesenchymal stem cell) characteristics, including multipotentiality, self-renewal, immunoregulatory functions, and diverse roles in tissue repair. Here, we investigate the differential but overlapping roles of two perivascular cell subsets in paracrine induction of bone repair. CD146(+)CD34(−)CD31(−)CD45(−)pericytes and CD34(+)CD146(−)CD31(−)CD45(−)adventitial cells were derived from human adipose tissue and applied alone or in combination to calvarial bone defects in mice. In vitro, osteogenic differentiation and tubulogenesis assays were performed using either fluorescence activated cell sorting-derived CD146(+) pericytes or CD34(+) adventitial cells. Results showed that CD146(+) pericytes induced increased cord formation in vitro and angiogenesis in vivo in comparison with patient-matched CD34(+) adventitial cells. In contrast, CD34(+) adventitial cells demonstrated heightened paracrine-induced osteogenesis in vitro. When applied in a critical-size calvarial defect model in NOD/SCID mice, the combination treatment of CD146(+) pericytes with CD34(+) adventitial cells led to greater re-ossification than either cell type alone. In summary, adipose-derived CD146(+) pericytes and CD34(+) adventitial cells display functionally distinct yet overlapping and complementary roles in bone defect repair. Consequently, CD146(+) pericytes and CD34(+) adventitial cells may demonstrate synergistic bone healing when applied as a combination cellular therapy. Nature Publishing Group UK 2019-01-07 /pmc/articles/PMC6323123/ /pubmed/30622740 http://dx.doi.org/10.1038/s41536-018-0063-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Wang, Yiyun Xu, Jiajia Chang, Leslie Meyers, Carolyn A. Zhang, Lei Broderick, Kristen Lee, Min Peault, Bruno James, Aaron W. Relative contributions of adipose-resident CD146(+) pericytes and CD34(+) adventitial progenitor cells in bone tissue engineering |
title | Relative contributions of adipose-resident CD146(+) pericytes and CD34(+) adventitial progenitor cells in bone tissue engineering |
title_full | Relative contributions of adipose-resident CD146(+) pericytes and CD34(+) adventitial progenitor cells in bone tissue engineering |
title_fullStr | Relative contributions of adipose-resident CD146(+) pericytes and CD34(+) adventitial progenitor cells in bone tissue engineering |
title_full_unstemmed | Relative contributions of adipose-resident CD146(+) pericytes and CD34(+) adventitial progenitor cells in bone tissue engineering |
title_short | Relative contributions of adipose-resident CD146(+) pericytes and CD34(+) adventitial progenitor cells in bone tissue engineering |
title_sort | relative contributions of adipose-resident cd146(+) pericytes and cd34(+) adventitial progenitor cells in bone tissue engineering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323123/ https://www.ncbi.nlm.nih.gov/pubmed/30622740 http://dx.doi.org/10.1038/s41536-018-0063-2 |
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