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In vitro dissolution and bioavailability study of furosemide nanosuspension prepared using design of experiment (DoE)

BACKGROUND: Nanotechnology can offer the advantages of increasing solubility and bioavailability of delivering drugs like Furosemide. The aim of the current study is to investigate the in vitro and in vivo performance of furosemide nanosuspensions. METHODS: Furosemide nanosuspensions were prepared b...

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Autores principales: Shariare, Mohammad H., Altamimi, Mohammad A., Marzan, Akbar L., Tabassum, Rahnuma, Jahan, Basarat, Reza, Hasan M., Rahman, Mahbubur, Ahsan, G.U., Kazi, Mohsin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323151/
https://www.ncbi.nlm.nih.gov/pubmed/30662312
http://dx.doi.org/10.1016/j.jsps.2018.09.002
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author Shariare, Mohammad H.
Altamimi, Mohammad A.
Marzan, Akbar L.
Tabassum, Rahnuma
Jahan, Basarat
Reza, Hasan M.
Rahman, Mahbubur
Ahsan, G.U.
Kazi, Mohsin
author_facet Shariare, Mohammad H.
Altamimi, Mohammad A.
Marzan, Akbar L.
Tabassum, Rahnuma
Jahan, Basarat
Reza, Hasan M.
Rahman, Mahbubur
Ahsan, G.U.
Kazi, Mohsin
author_sort Shariare, Mohammad H.
collection PubMed
description BACKGROUND: Nanotechnology can offer the advantages of increasing solubility and bioavailability of delivering drugs like Furosemide. The aim of the current study is to investigate the in vitro and in vivo performance of furosemide nanosuspensions. METHODS: Furosemide nanosuspensions were prepared by antisolvent precipitation method using full factorial experimental design. Four factors were employed namely; Stirring time, Injection rate, antisolvent: solvent ratio & stabilizer: drug ratio (at two levels = high & low). The in vitro dissolution experiments were conducted to compare the representative formulation with raw drug powder. The bioavailability of nanosuspension was, also, evaluated in mice as an animal model. RESULTS: Solid state characterization (PXRD, DSC and FESEM) did show physical changes during preparation and optimization of the furosemide nanosuspensions. Individual material attributes showed more significant impact on the average particle size of the nanocrystals compared to process parameters. Two-way interactions between material attributes and process parameters significantly affected nanosuspension particle size distribution. Dissolution rate of furosemide nanosuspemsion was significantly higher than that observed for raw furosemide powder. The in vivo pharmacokinetics parameters of nanosuspension in comparison to pure drug showed significant increase in C(max) and AUC((0-t),) about 233% and 266%, respectively. The oral bioavailability of furosemide from nanosuspension was about 2.3 fold higher as compared with the bioavailability from pure drug. CONCLUSIONS: Furosemide nanosuspensions prepared using antisolvent precipitation method enhanced the dissolution rate and oral bioavailability compared to raw furosemide powder.
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spelling pubmed-63231512019-01-18 In vitro dissolution and bioavailability study of furosemide nanosuspension prepared using design of experiment (DoE) Shariare, Mohammad H. Altamimi, Mohammad A. Marzan, Akbar L. Tabassum, Rahnuma Jahan, Basarat Reza, Hasan M. Rahman, Mahbubur Ahsan, G.U. Kazi, Mohsin Saudi Pharm J Article BACKGROUND: Nanotechnology can offer the advantages of increasing solubility and bioavailability of delivering drugs like Furosemide. The aim of the current study is to investigate the in vitro and in vivo performance of furosemide nanosuspensions. METHODS: Furosemide nanosuspensions were prepared by antisolvent precipitation method using full factorial experimental design. Four factors were employed namely; Stirring time, Injection rate, antisolvent: solvent ratio & stabilizer: drug ratio (at two levels = high & low). The in vitro dissolution experiments were conducted to compare the representative formulation with raw drug powder. The bioavailability of nanosuspension was, also, evaluated in mice as an animal model. RESULTS: Solid state characterization (PXRD, DSC and FESEM) did show physical changes during preparation and optimization of the furosemide nanosuspensions. Individual material attributes showed more significant impact on the average particle size of the nanocrystals compared to process parameters. Two-way interactions between material attributes and process parameters significantly affected nanosuspension particle size distribution. Dissolution rate of furosemide nanosuspemsion was significantly higher than that observed for raw furosemide powder. The in vivo pharmacokinetics parameters of nanosuspension in comparison to pure drug showed significant increase in C(max) and AUC((0-t),) about 233% and 266%, respectively. The oral bioavailability of furosemide from nanosuspension was about 2.3 fold higher as compared with the bioavailability from pure drug. CONCLUSIONS: Furosemide nanosuspensions prepared using antisolvent precipitation method enhanced the dissolution rate and oral bioavailability compared to raw furosemide powder. Elsevier 2019-01 2018-09-03 /pmc/articles/PMC6323151/ /pubmed/30662312 http://dx.doi.org/10.1016/j.jsps.2018.09.002 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Shariare, Mohammad H.
Altamimi, Mohammad A.
Marzan, Akbar L.
Tabassum, Rahnuma
Jahan, Basarat
Reza, Hasan M.
Rahman, Mahbubur
Ahsan, G.U.
Kazi, Mohsin
In vitro dissolution and bioavailability study of furosemide nanosuspension prepared using design of experiment (DoE)
title In vitro dissolution and bioavailability study of furosemide nanosuspension prepared using design of experiment (DoE)
title_full In vitro dissolution and bioavailability study of furosemide nanosuspension prepared using design of experiment (DoE)
title_fullStr In vitro dissolution and bioavailability study of furosemide nanosuspension prepared using design of experiment (DoE)
title_full_unstemmed In vitro dissolution and bioavailability study of furosemide nanosuspension prepared using design of experiment (DoE)
title_short In vitro dissolution and bioavailability study of furosemide nanosuspension prepared using design of experiment (DoE)
title_sort in vitro dissolution and bioavailability study of furosemide nanosuspension prepared using design of experiment (doe)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323151/
https://www.ncbi.nlm.nih.gov/pubmed/30662312
http://dx.doi.org/10.1016/j.jsps.2018.09.002
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