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An experimental study of amniotic lacrimal duct stents in the treatment of perimenopausal female rabbits with dry eye

The aim of the present study was to investigate the effects of an amniotic membrane lacrimal stent in the treatment of dry eye syndrome in a perimenopausal rabbit model. In total, 48 healthy female rabbits were randomly divided into four groups (12 rabbits/group): Group A, sham-operated group; group...

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Autores principales: Ma, Mingyang, Yuan, Qing, Ye, Lei, Liu, Kangcheng, Ye, Linhong, Min, You-Lan, Jiang, Nan, Li, Qinghai, Shi, Wenqing, Xu, Xiaowei, Zhu, Peiwen, Shao, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323207/
https://www.ncbi.nlm.nih.gov/pubmed/30569093
http://dx.doi.org/10.3892/mmr.2018.9731
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author Ma, Mingyang
Yuan, Qing
Ye, Lei
Liu, Kangcheng
Ye, Linhong
Min, You-Lan
Jiang, Nan
Li, Qinghai
Shi, Wenqing
Xu, Xiaowei
Zhu, Peiwen
Shao, Yi
author_facet Ma, Mingyang
Yuan, Qing
Ye, Lei
Liu, Kangcheng
Ye, Linhong
Min, You-Lan
Jiang, Nan
Li, Qinghai
Shi, Wenqing
Xu, Xiaowei
Zhu, Peiwen
Shao, Yi
author_sort Ma, Mingyang
collection PubMed
description The aim of the present study was to investigate the effects of an amniotic membrane lacrimal stent in the treatment of dry eye syndrome in a perimenopausal rabbit model. In total, 48 healthy female rabbits were randomly divided into four groups (12 rabbits/group): Group A, sham-operated group; group B, negative control group; group C, sham-implantation group; and group D, implantation with amniotic membrane lacrimal stents. A Schirmer I test (SIT), corneal fluorescein staining, optical coherence tomography angiography and corneal confocal microscopy were conducted and the biomechanical properties of the amniotic membrane were measured prior to and 2, 4 and 6 weeks after the operations. There were marked differences in the amount of tear secretion and the SIT following the operation compared with prior to the operation. The amount of tear secretion of group B and C was significantly reduced (F=8.894; P=0.0017). Compared with groups A, B and C, group D demonstrated a significant increase in the amount of tear secretion and a significant reduction in SIT (P<0.05). However, there was no statistical difference between the tear secretion of the A, B and C groups (P>0.05). The cornea epithelial of groups A and D was significantly thinner compared with groups B and C. The superior part [superior temporal (ST)5, superior (S)5, superior nasal (SN)5, ST6, S6 and SN6] was significantly thinner compared with the central part of the cornea epithelium. The corneal epithelia of all groups were thin in the center and thick at the edge. At 6 weeks after the operation, group D exhibited a markedly decreased number of anterior stromal luminescent cells, compared with groups A, B and C. Furthermore, the bending degree of corneal stromal neural trunks was significantly improved, and the density, branches and curvature of corneal epithelium sub-basal nerves ameliorated to a certain extent (P<0.05). Amniotic membrane lacrimal stents demonstrated specific therapeutic effects on dry eye in perimenopausal female rabbits.
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spelling pubmed-63232072019-01-15 An experimental study of amniotic lacrimal duct stents in the treatment of perimenopausal female rabbits with dry eye Ma, Mingyang Yuan, Qing Ye, Lei Liu, Kangcheng Ye, Linhong Min, You-Lan Jiang, Nan Li, Qinghai Shi, Wenqing Xu, Xiaowei Zhu, Peiwen Shao, Yi Mol Med Rep Articles The aim of the present study was to investigate the effects of an amniotic membrane lacrimal stent in the treatment of dry eye syndrome in a perimenopausal rabbit model. In total, 48 healthy female rabbits were randomly divided into four groups (12 rabbits/group): Group A, sham-operated group; group B, negative control group; group C, sham-implantation group; and group D, implantation with amniotic membrane lacrimal stents. A Schirmer I test (SIT), corneal fluorescein staining, optical coherence tomography angiography and corneal confocal microscopy were conducted and the biomechanical properties of the amniotic membrane were measured prior to and 2, 4 and 6 weeks after the operations. There were marked differences in the amount of tear secretion and the SIT following the operation compared with prior to the operation. The amount of tear secretion of group B and C was significantly reduced (F=8.894; P=0.0017). Compared with groups A, B and C, group D demonstrated a significant increase in the amount of tear secretion and a significant reduction in SIT (P<0.05). However, there was no statistical difference between the tear secretion of the A, B and C groups (P>0.05). The cornea epithelial of groups A and D was significantly thinner compared with groups B and C. The superior part [superior temporal (ST)5, superior (S)5, superior nasal (SN)5, ST6, S6 and SN6] was significantly thinner compared with the central part of the cornea epithelium. The corneal epithelia of all groups were thin in the center and thick at the edge. At 6 weeks after the operation, group D exhibited a markedly decreased number of anterior stromal luminescent cells, compared with groups A, B and C. Furthermore, the bending degree of corneal stromal neural trunks was significantly improved, and the density, branches and curvature of corneal epithelium sub-basal nerves ameliorated to a certain extent (P<0.05). Amniotic membrane lacrimal stents demonstrated specific therapeutic effects on dry eye in perimenopausal female rabbits. D.A. Spandidos 2019-02 2018-12-10 /pmc/articles/PMC6323207/ /pubmed/30569093 http://dx.doi.org/10.3892/mmr.2018.9731 Text en Copyright: © Ma et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Ma, Mingyang
Yuan, Qing
Ye, Lei
Liu, Kangcheng
Ye, Linhong
Min, You-Lan
Jiang, Nan
Li, Qinghai
Shi, Wenqing
Xu, Xiaowei
Zhu, Peiwen
Shao, Yi
An experimental study of amniotic lacrimal duct stents in the treatment of perimenopausal female rabbits with dry eye
title An experimental study of amniotic lacrimal duct stents in the treatment of perimenopausal female rabbits with dry eye
title_full An experimental study of amniotic lacrimal duct stents in the treatment of perimenopausal female rabbits with dry eye
title_fullStr An experimental study of amniotic lacrimal duct stents in the treatment of perimenopausal female rabbits with dry eye
title_full_unstemmed An experimental study of amniotic lacrimal duct stents in the treatment of perimenopausal female rabbits with dry eye
title_short An experimental study of amniotic lacrimal duct stents in the treatment of perimenopausal female rabbits with dry eye
title_sort experimental study of amniotic lacrimal duct stents in the treatment of perimenopausal female rabbits with dry eye
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323207/
https://www.ncbi.nlm.nih.gov/pubmed/30569093
http://dx.doi.org/10.3892/mmr.2018.9731
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