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Long noncoding RNA RUSC1-AS-N promotes cell proliferation and metastasis through Wnt/β-catenin signaling in human breast cancer

Breast cancer is one of the most frequently diagnosed cancers among females worldwide. Long noncoding RNAs (lncRNAs) have been revealed to serve significant roles in diagnosis and treatment of breast cancer. In the present study, the novel lncRNA RUSC1-AS-N was demonstrated to promote cell viability...

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Autores principales: Zhou, Peng, Liu, Peng, Zhang, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323231/
https://www.ncbi.nlm.nih.gov/pubmed/30569097
http://dx.doi.org/10.3892/mmr.2018.9763
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author Zhou, Peng
Liu, Peng
Zhang, Jin
author_facet Zhou, Peng
Liu, Peng
Zhang, Jin
author_sort Zhou, Peng
collection PubMed
description Breast cancer is one of the most frequently diagnosed cancers among females worldwide. Long noncoding RNAs (lncRNAs) have been revealed to serve significant roles in diagnosis and treatment of breast cancer. In the present study, the novel lncRNA RUSC1-AS-N was demonstrated to promote cell viability and metastasis. A total of 100 patients with breast cancer were recruited for this study and it was revealed that RUSC1-AS-N was upregulated in tumor tissues compared with in adjacent non-cancerous counterparts. In addition, using several breast cancer cell lines, it was demonstrated that the mRNA levels of RUSC1-AS-N were highest in the notably metastatic cell lines MDA-MB-231 and MDA-MB-468. Knockdown of RUSC1-AS-N in breast cancer cells inhibited cell proliferation in the colony formation and cell proliferation assays. Furthermore, depletion of RUSC1-AS-N suppressed cell metastasis, as revealed by wound-healing and western blot assays. In addition, the protein levels of Wnt1 and β-catenin were significantly decreased when RUSC1-AS-N was knocked down. However, Wnt signaling pathway activator Wnt agonist 1 reversed the effects of RUSC1-AS-N knockdown on cell proliferation and metastasis. The present study demonstrated that lncRNA RUSC1-AS-N promoted cell viability and metastasis via Wnt/β-catenin signaling in human breast cancer, which may indicate novel targets for the treatment of breast cancer in clinic.
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spelling pubmed-63232312019-01-15 Long noncoding RNA RUSC1-AS-N promotes cell proliferation and metastasis through Wnt/β-catenin signaling in human breast cancer Zhou, Peng Liu, Peng Zhang, Jin Mol Med Rep Articles Breast cancer is one of the most frequently diagnosed cancers among females worldwide. Long noncoding RNAs (lncRNAs) have been revealed to serve significant roles in diagnosis and treatment of breast cancer. In the present study, the novel lncRNA RUSC1-AS-N was demonstrated to promote cell viability and metastasis. A total of 100 patients with breast cancer were recruited for this study and it was revealed that RUSC1-AS-N was upregulated in tumor tissues compared with in adjacent non-cancerous counterparts. In addition, using several breast cancer cell lines, it was demonstrated that the mRNA levels of RUSC1-AS-N were highest in the notably metastatic cell lines MDA-MB-231 and MDA-MB-468. Knockdown of RUSC1-AS-N in breast cancer cells inhibited cell proliferation in the colony formation and cell proliferation assays. Furthermore, depletion of RUSC1-AS-N suppressed cell metastasis, as revealed by wound-healing and western blot assays. In addition, the protein levels of Wnt1 and β-catenin were significantly decreased when RUSC1-AS-N was knocked down. However, Wnt signaling pathway activator Wnt agonist 1 reversed the effects of RUSC1-AS-N knockdown on cell proliferation and metastasis. The present study demonstrated that lncRNA RUSC1-AS-N promoted cell viability and metastasis via Wnt/β-catenin signaling in human breast cancer, which may indicate novel targets for the treatment of breast cancer in clinic. D.A. Spandidos 2019-02 2018-12-14 /pmc/articles/PMC6323231/ /pubmed/30569097 http://dx.doi.org/10.3892/mmr.2018.9763 Text en Copyright: © Zhou et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhou, Peng
Liu, Peng
Zhang, Jin
Long noncoding RNA RUSC1-AS-N promotes cell proliferation and metastasis through Wnt/β-catenin signaling in human breast cancer
title Long noncoding RNA RUSC1-AS-N promotes cell proliferation and metastasis through Wnt/β-catenin signaling in human breast cancer
title_full Long noncoding RNA RUSC1-AS-N promotes cell proliferation and metastasis through Wnt/β-catenin signaling in human breast cancer
title_fullStr Long noncoding RNA RUSC1-AS-N promotes cell proliferation and metastasis through Wnt/β-catenin signaling in human breast cancer
title_full_unstemmed Long noncoding RNA RUSC1-AS-N promotes cell proliferation and metastasis through Wnt/β-catenin signaling in human breast cancer
title_short Long noncoding RNA RUSC1-AS-N promotes cell proliferation and metastasis through Wnt/β-catenin signaling in human breast cancer
title_sort long noncoding rna rusc1-as-n promotes cell proliferation and metastasis through wnt/β-catenin signaling in human breast cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323231/
https://www.ncbi.nlm.nih.gov/pubmed/30569097
http://dx.doi.org/10.3892/mmr.2018.9763
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