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The Removal of HBV in Plasma by Extracorporeal Immunoadsorption from Plasma: A Potential Therapy of Hepatitis B Patients

OBJECTIVE: To establish a novel HBV specific immunoadsorbent for the removing of HBV particles. METHODS: The anti-HBsAg monoclonal antibody was immobilized on sepharose beads to produce a sepharose anti-HBs column. Then the immunoadsorbent was evaluated and characterized by scanning electron microsc...

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Detalles Bibliográficos
Autores principales: Han, Zhenwei, Lu, Xuan, Tang, Yiping, Yang, Yuanyuan, Liu, Qiuchen, Cheng, Pengfei, Zhou, Li, Wang, Yefu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323487/
https://www.ncbi.nlm.nih.gov/pubmed/30671444
http://dx.doi.org/10.1155/2018/1269063
Descripción
Sumario:OBJECTIVE: To establish a novel HBV specific immunoadsorbent for the removing of HBV particles. METHODS: The anti-HBsAg monoclonal antibody was immobilized on sepharose beads to produce a sepharose anti-HBs column. Then the immunoadsorbent was evaluated and characterized by scanning electron microscopy. In addition, time-dependent effects of the eradication capacity of anti-HBsAg functionalized sepharose beads against HBV were investigated. RESULTS: Proposed immunoadsorbents exhibited a favorable biocompatibility as well as specificity. With the optimized recycle time, the decontamination performance of HBV particles and quantity of HBsAg were assessed either by real-time quantitative PCR or ELISA, which showed that the immunoadsorbent could remove approximately 90% of the HBV and 90% of the HBsAg from human plasma samples. CONCLUSIONS: All these results indicated that the novel immunoadsorbent could effectively remove HBV particles and likely serve as a novel therapy option or at least supplementary for the treatment regimen of HBV.