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Danggui Buxue Tang, an ancient Chinese herbal decoction, protects β-amyloid-induced cell death in cultured cortical neurons

BACKGROUND: Danggui Buxue Tang (DBT) is a historical Chinese herbal decoction, and which has more than 800 years of applications. This herbal decoction solely contains two materials: Astragali Radix (AR) and Angelicae Sinensis Radix (ASR) at a weight ratio of 5:1. Clinically, DBT aims to improve ane...

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Autores principales: Gong, Guowei, Qi, Baohui, Liang, Yan T., Dong, Tina T. X., Wang, Huai Y., Tsim, Karl W. K., Zheng, Yuzhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323849/
https://www.ncbi.nlm.nih.gov/pubmed/30621672
http://dx.doi.org/10.1186/s12906-018-2411-6
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author Gong, Guowei
Qi, Baohui
Liang, Yan T.
Dong, Tina T. X.
Wang, Huai Y.
Tsim, Karl W. K.
Zheng, Yuzhong
author_facet Gong, Guowei
Qi, Baohui
Liang, Yan T.
Dong, Tina T. X.
Wang, Huai Y.
Tsim, Karl W. K.
Zheng, Yuzhong
author_sort Gong, Guowei
collection PubMed
description BACKGROUND: Danggui Buxue Tang (DBT) is a historical Chinese herbal decoction, and which has more than 800 years of applications. This herbal decoction solely contains two materials: Astragali Radix (AR) and Angelicae Sinensis Radix (ASR) at a weight ratio of 5:1. Clinically, DBT aims to improve anemia syndrome. In complementary and alternative medicine theory, the cause of neurodegenerative disease is proposed to be related with anemia. In line to this notion, low levels of hemoglobin and red blood cell have been reported in patients suffering from Alzheimer’s disease (AD), a chronic neurodegenerative disease caused by β-amyloid peptide (Aβ) accumulation. Therefore, we would like to probe the neuroprotective functions of this ancient herbal formula in vitro. METHOD: The neuroprotective effects of DBT in the Aβ-induced cell death were detected in cultured cortical neurons by multiple techniques, i.e. confocal and western blot. RESULTS: In the cultures, application of DBT reduced Aβ-induced apoptosis rate in a dose-dependent manner. In Aβ-treated cortical neurons, the expression ratio of Bcl2 to Bax was altered by DBT. In parallel, application of DBT markedly suppressed the Aβ-induced expressions of apoptotic markers, i.e. cleaved-caspase 3/9 and PARP. CONCLUSION: Taken these results, DBT shows promising protective effects against Aβ-induced stress or insult in cultured neurons.
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spelling pubmed-63238492019-01-11 Danggui Buxue Tang, an ancient Chinese herbal decoction, protects β-amyloid-induced cell death in cultured cortical neurons Gong, Guowei Qi, Baohui Liang, Yan T. Dong, Tina T. X. Wang, Huai Y. Tsim, Karl W. K. Zheng, Yuzhong BMC Complement Altern Med Research Article BACKGROUND: Danggui Buxue Tang (DBT) is a historical Chinese herbal decoction, and which has more than 800 years of applications. This herbal decoction solely contains two materials: Astragali Radix (AR) and Angelicae Sinensis Radix (ASR) at a weight ratio of 5:1. Clinically, DBT aims to improve anemia syndrome. In complementary and alternative medicine theory, the cause of neurodegenerative disease is proposed to be related with anemia. In line to this notion, low levels of hemoglobin and red blood cell have been reported in patients suffering from Alzheimer’s disease (AD), a chronic neurodegenerative disease caused by β-amyloid peptide (Aβ) accumulation. Therefore, we would like to probe the neuroprotective functions of this ancient herbal formula in vitro. METHOD: The neuroprotective effects of DBT in the Aβ-induced cell death were detected in cultured cortical neurons by multiple techniques, i.e. confocal and western blot. RESULTS: In the cultures, application of DBT reduced Aβ-induced apoptosis rate in a dose-dependent manner. In Aβ-treated cortical neurons, the expression ratio of Bcl2 to Bax was altered by DBT. In parallel, application of DBT markedly suppressed the Aβ-induced expressions of apoptotic markers, i.e. cleaved-caspase 3/9 and PARP. CONCLUSION: Taken these results, DBT shows promising protective effects against Aβ-induced stress or insult in cultured neurons. BioMed Central 2019-01-08 /pmc/articles/PMC6323849/ /pubmed/30621672 http://dx.doi.org/10.1186/s12906-018-2411-6 Text en © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Gong, Guowei
Qi, Baohui
Liang, Yan T.
Dong, Tina T. X.
Wang, Huai Y.
Tsim, Karl W. K.
Zheng, Yuzhong
Danggui Buxue Tang, an ancient Chinese herbal decoction, protects β-amyloid-induced cell death in cultured cortical neurons
title Danggui Buxue Tang, an ancient Chinese herbal decoction, protects β-amyloid-induced cell death in cultured cortical neurons
title_full Danggui Buxue Tang, an ancient Chinese herbal decoction, protects β-amyloid-induced cell death in cultured cortical neurons
title_fullStr Danggui Buxue Tang, an ancient Chinese herbal decoction, protects β-amyloid-induced cell death in cultured cortical neurons
title_full_unstemmed Danggui Buxue Tang, an ancient Chinese herbal decoction, protects β-amyloid-induced cell death in cultured cortical neurons
title_short Danggui Buxue Tang, an ancient Chinese herbal decoction, protects β-amyloid-induced cell death in cultured cortical neurons
title_sort danggui buxue tang, an ancient chinese herbal decoction, protects β-amyloid-induced cell death in cultured cortical neurons
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323849/
https://www.ncbi.nlm.nih.gov/pubmed/30621672
http://dx.doi.org/10.1186/s12906-018-2411-6
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