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Dopamine levels in human tear fluid

PURPOSE: To determine the levels of dopamine in tear fluid and demonstrate the use of tear fluid as a non-invasive source for dopamine measurements in humans. METHODS: The study cohort included 30 clinically healthy individuals without any pre-existing ocular or systemic conditions. Matched tear flu...

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Autores principales: Sharma, Niyati Seshagiri, Acharya, Suraj Kumar, Nair, Archana Padmanabhan, Matalia, Jyoti, Shetty, Rohit, Ghosh, Arkasubhra, Sethu, Swaminathan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324103/
https://www.ncbi.nlm.nih.gov/pubmed/30574889
http://dx.doi.org/10.4103/ijo.IJO_568_18
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author Sharma, Niyati Seshagiri
Acharya, Suraj Kumar
Nair, Archana Padmanabhan
Matalia, Jyoti
Shetty, Rohit
Ghosh, Arkasubhra
Sethu, Swaminathan
author_facet Sharma, Niyati Seshagiri
Acharya, Suraj Kumar
Nair, Archana Padmanabhan
Matalia, Jyoti
Shetty, Rohit
Ghosh, Arkasubhra
Sethu, Swaminathan
author_sort Sharma, Niyati Seshagiri
collection PubMed
description PURPOSE: To determine the levels of dopamine in tear fluid and demonstrate the use of tear fluid as a non-invasive source for dopamine measurements in humans. METHODS: The study cohort included 30 clinically healthy individuals without any pre-existing ocular or systemic conditions. Matched tear fluid (using Schirmer's strips and capillary tubes) and plasma were collected from the subjects. Dopamine levels were evaluated using direct competitive chemiluminescent enzyme-linked immunosorbent assay (ELISA), dopamine kit (Cloud Clone Corp, TX, USA). RESULTS: Significantly higher dopamine levels were found in the tear fluid compared to plasma in the study subjects. The level of dopamine was 97.2 ± 11.80 pg/ml (mean ± SEM), 279 ± 14.8 pg/ml (mean ± SEM), and 470.4 ± 37.64 pg/ml (mean ± SEM) in the plasma and in the tears collected using Schirmer's strips and capillary tubes, respectively. CONCLUSION: Dopamine was detectable in all the tear fluid samples tested and was also found to be at a higher concentration than in plasma samples. Tear fluid can be used as a non-invasive sample source to monitor dopamine levels.
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spelling pubmed-63241032019-01-25 Dopamine levels in human tear fluid Sharma, Niyati Seshagiri Acharya, Suraj Kumar Nair, Archana Padmanabhan Matalia, Jyoti Shetty, Rohit Ghosh, Arkasubhra Sethu, Swaminathan Indian J Ophthalmol Original Article PURPOSE: To determine the levels of dopamine in tear fluid and demonstrate the use of tear fluid as a non-invasive source for dopamine measurements in humans. METHODS: The study cohort included 30 clinically healthy individuals without any pre-existing ocular or systemic conditions. Matched tear fluid (using Schirmer's strips and capillary tubes) and plasma were collected from the subjects. Dopamine levels were evaluated using direct competitive chemiluminescent enzyme-linked immunosorbent assay (ELISA), dopamine kit (Cloud Clone Corp, TX, USA). RESULTS: Significantly higher dopamine levels were found in the tear fluid compared to plasma in the study subjects. The level of dopamine was 97.2 ± 11.80 pg/ml (mean ± SEM), 279 ± 14.8 pg/ml (mean ± SEM), and 470.4 ± 37.64 pg/ml (mean ± SEM) in the plasma and in the tears collected using Schirmer's strips and capillary tubes, respectively. CONCLUSION: Dopamine was detectable in all the tear fluid samples tested and was also found to be at a higher concentration than in plasma samples. Tear fluid can be used as a non-invasive sample source to monitor dopamine levels. Medknow Publications & Media Pvt Ltd 2019-01 /pmc/articles/PMC6324103/ /pubmed/30574889 http://dx.doi.org/10.4103/ijo.IJO_568_18 Text en Copyright: © 2018 Indian Journal of Ophthalmology http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Sharma, Niyati Seshagiri
Acharya, Suraj Kumar
Nair, Archana Padmanabhan
Matalia, Jyoti
Shetty, Rohit
Ghosh, Arkasubhra
Sethu, Swaminathan
Dopamine levels in human tear fluid
title Dopamine levels in human tear fluid
title_full Dopamine levels in human tear fluid
title_fullStr Dopamine levels in human tear fluid
title_full_unstemmed Dopamine levels in human tear fluid
title_short Dopamine levels in human tear fluid
title_sort dopamine levels in human tear fluid
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324103/
https://www.ncbi.nlm.nih.gov/pubmed/30574889
http://dx.doi.org/10.4103/ijo.IJO_568_18
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