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Dopamine levels in human tear fluid
PURPOSE: To determine the levels of dopamine in tear fluid and demonstrate the use of tear fluid as a non-invasive source for dopamine measurements in humans. METHODS: The study cohort included 30 clinically healthy individuals without any pre-existing ocular or systemic conditions. Matched tear flu...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324103/ https://www.ncbi.nlm.nih.gov/pubmed/30574889 http://dx.doi.org/10.4103/ijo.IJO_568_18 |
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author | Sharma, Niyati Seshagiri Acharya, Suraj Kumar Nair, Archana Padmanabhan Matalia, Jyoti Shetty, Rohit Ghosh, Arkasubhra Sethu, Swaminathan |
author_facet | Sharma, Niyati Seshagiri Acharya, Suraj Kumar Nair, Archana Padmanabhan Matalia, Jyoti Shetty, Rohit Ghosh, Arkasubhra Sethu, Swaminathan |
author_sort | Sharma, Niyati Seshagiri |
collection | PubMed |
description | PURPOSE: To determine the levels of dopamine in tear fluid and demonstrate the use of tear fluid as a non-invasive source for dopamine measurements in humans. METHODS: The study cohort included 30 clinically healthy individuals without any pre-existing ocular or systemic conditions. Matched tear fluid (using Schirmer's strips and capillary tubes) and plasma were collected from the subjects. Dopamine levels were evaluated using direct competitive chemiluminescent enzyme-linked immunosorbent assay (ELISA), dopamine kit (Cloud Clone Corp, TX, USA). RESULTS: Significantly higher dopamine levels were found in the tear fluid compared to plasma in the study subjects. The level of dopamine was 97.2 ± 11.80 pg/ml (mean ± SEM), 279 ± 14.8 pg/ml (mean ± SEM), and 470.4 ± 37.64 pg/ml (mean ± SEM) in the plasma and in the tears collected using Schirmer's strips and capillary tubes, respectively. CONCLUSION: Dopamine was detectable in all the tear fluid samples tested and was also found to be at a higher concentration than in plasma samples. Tear fluid can be used as a non-invasive sample source to monitor dopamine levels. |
format | Online Article Text |
id | pubmed-6324103 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-63241032019-01-25 Dopamine levels in human tear fluid Sharma, Niyati Seshagiri Acharya, Suraj Kumar Nair, Archana Padmanabhan Matalia, Jyoti Shetty, Rohit Ghosh, Arkasubhra Sethu, Swaminathan Indian J Ophthalmol Original Article PURPOSE: To determine the levels of dopamine in tear fluid and demonstrate the use of tear fluid as a non-invasive source for dopamine measurements in humans. METHODS: The study cohort included 30 clinically healthy individuals without any pre-existing ocular or systemic conditions. Matched tear fluid (using Schirmer's strips and capillary tubes) and plasma were collected from the subjects. Dopamine levels were evaluated using direct competitive chemiluminescent enzyme-linked immunosorbent assay (ELISA), dopamine kit (Cloud Clone Corp, TX, USA). RESULTS: Significantly higher dopamine levels were found in the tear fluid compared to plasma in the study subjects. The level of dopamine was 97.2 ± 11.80 pg/ml (mean ± SEM), 279 ± 14.8 pg/ml (mean ± SEM), and 470.4 ± 37.64 pg/ml (mean ± SEM) in the plasma and in the tears collected using Schirmer's strips and capillary tubes, respectively. CONCLUSION: Dopamine was detectable in all the tear fluid samples tested and was also found to be at a higher concentration than in plasma samples. Tear fluid can be used as a non-invasive sample source to monitor dopamine levels. Medknow Publications & Media Pvt Ltd 2019-01 /pmc/articles/PMC6324103/ /pubmed/30574889 http://dx.doi.org/10.4103/ijo.IJO_568_18 Text en Copyright: © 2018 Indian Journal of Ophthalmology http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Sharma, Niyati Seshagiri Acharya, Suraj Kumar Nair, Archana Padmanabhan Matalia, Jyoti Shetty, Rohit Ghosh, Arkasubhra Sethu, Swaminathan Dopamine levels in human tear fluid |
title | Dopamine levels in human tear fluid |
title_full | Dopamine levels in human tear fluid |
title_fullStr | Dopamine levels in human tear fluid |
title_full_unstemmed | Dopamine levels in human tear fluid |
title_short | Dopamine levels in human tear fluid |
title_sort | dopamine levels in human tear fluid |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324103/ https://www.ncbi.nlm.nih.gov/pubmed/30574889 http://dx.doi.org/10.4103/ijo.IJO_568_18 |
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