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A rapid method for determining protein diffusion through hydrogels for regenerative medicine applications

Hydrogels present versatile platforms for the encapsulation and delivery of proteins and cells for regenerative medicine applications. However, differences in hydrogel cross-linking density, polymer weight content, and affinity for proteins all contribute to diverse diffusion rates of proteins throu...

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Detalles Bibliográficos
Autores principales: Hettiaratchi, Marian H., Schudel, Alex, Rouse, Tel, García, Andrés J., Thomas, Susan N., Guldberg, Robert E., McDevitt, Todd C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324205/
https://www.ncbi.nlm.nih.gov/pubmed/31069307
http://dx.doi.org/10.1063/1.4999925
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author Hettiaratchi, Marian H.
Schudel, Alex
Rouse, Tel
García, Andrés J.
Thomas, Susan N.
Guldberg, Robert E.
McDevitt, Todd C.
author_facet Hettiaratchi, Marian H.
Schudel, Alex
Rouse, Tel
García, Andrés J.
Thomas, Susan N.
Guldberg, Robert E.
McDevitt, Todd C.
author_sort Hettiaratchi, Marian H.
collection PubMed
description Hydrogels present versatile platforms for the encapsulation and delivery of proteins and cells for regenerative medicine applications. However, differences in hydrogel cross-linking density, polymer weight content, and affinity for proteins all contribute to diverse diffusion rates of proteins through hydrogel networks. Here, we describe a simple method to accurately measure protein diffusion through hydrogels, within a few hours and without the use of large amounts of protein. We tracked the diffusion of several proteins of varying molecular weights along the axial direction of capillary tubes filled with alginate, collagen, or poly(ethylene glycol) hydrogels. The rate of protein diffusion decreased with increasing molecular weight. A computational model of protein diffusion through capillary tubes was also created to predict and verify experimental protein diffusion coefficients. This in vitro capillary tube-based method of measuring protein diffusion represents a simple strategy to interrogate protein diffusion through natural and synthetic hydrogels and aid in the design of better biomaterial-based delivery vehicles that can effectively modulate protein release.
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spelling pubmed-63242052019-05-08 A rapid method for determining protein diffusion through hydrogels for regenerative medicine applications Hettiaratchi, Marian H. Schudel, Alex Rouse, Tel García, Andrés J. Thomas, Susan N. Guldberg, Robert E. McDevitt, Todd C. APL Bioeng Articles Hydrogels present versatile platforms for the encapsulation and delivery of proteins and cells for regenerative medicine applications. However, differences in hydrogel cross-linking density, polymer weight content, and affinity for proteins all contribute to diverse diffusion rates of proteins through hydrogel networks. Here, we describe a simple method to accurately measure protein diffusion through hydrogels, within a few hours and without the use of large amounts of protein. We tracked the diffusion of several proteins of varying molecular weights along the axial direction of capillary tubes filled with alginate, collagen, or poly(ethylene glycol) hydrogels. The rate of protein diffusion decreased with increasing molecular weight. A computational model of protein diffusion through capillary tubes was also created to predict and verify experimental protein diffusion coefficients. This in vitro capillary tube-based method of measuring protein diffusion represents a simple strategy to interrogate protein diffusion through natural and synthetic hydrogels and aid in the design of better biomaterial-based delivery vehicles that can effectively modulate protein release. AIP Publishing LLC 2018-06-12 /pmc/articles/PMC6324205/ /pubmed/31069307 http://dx.doi.org/10.1063/1.4999925 Text en © 2018 Author(s). 2473-2877/2018/2(2)/026110/14 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Articles
Hettiaratchi, Marian H.
Schudel, Alex
Rouse, Tel
García, Andrés J.
Thomas, Susan N.
Guldberg, Robert E.
McDevitt, Todd C.
A rapid method for determining protein diffusion through hydrogels for regenerative medicine applications
title A rapid method for determining protein diffusion through hydrogels for regenerative medicine applications
title_full A rapid method for determining protein diffusion through hydrogels for regenerative medicine applications
title_fullStr A rapid method for determining protein diffusion through hydrogels for regenerative medicine applications
title_full_unstemmed A rapid method for determining protein diffusion through hydrogels for regenerative medicine applications
title_short A rapid method for determining protein diffusion through hydrogels for regenerative medicine applications
title_sort rapid method for determining protein diffusion through hydrogels for regenerative medicine applications
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324205/
https://www.ncbi.nlm.nih.gov/pubmed/31069307
http://dx.doi.org/10.1063/1.4999925
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