Cargando…
A rapid method for determining protein diffusion through hydrogels for regenerative medicine applications
Hydrogels present versatile platforms for the encapsulation and delivery of proteins and cells for regenerative medicine applications. However, differences in hydrogel cross-linking density, polymer weight content, and affinity for proteins all contribute to diverse diffusion rates of proteins throu...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIP Publishing LLC
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324205/ https://www.ncbi.nlm.nih.gov/pubmed/31069307 http://dx.doi.org/10.1063/1.4999925 |
_version_ | 1783385928504442880 |
---|---|
author | Hettiaratchi, Marian H. Schudel, Alex Rouse, Tel García, Andrés J. Thomas, Susan N. Guldberg, Robert E. McDevitt, Todd C. |
author_facet | Hettiaratchi, Marian H. Schudel, Alex Rouse, Tel García, Andrés J. Thomas, Susan N. Guldberg, Robert E. McDevitt, Todd C. |
author_sort | Hettiaratchi, Marian H. |
collection | PubMed |
description | Hydrogels present versatile platforms for the encapsulation and delivery of proteins and cells for regenerative medicine applications. However, differences in hydrogel cross-linking density, polymer weight content, and affinity for proteins all contribute to diverse diffusion rates of proteins through hydrogel networks. Here, we describe a simple method to accurately measure protein diffusion through hydrogels, within a few hours and without the use of large amounts of protein. We tracked the diffusion of several proteins of varying molecular weights along the axial direction of capillary tubes filled with alginate, collagen, or poly(ethylene glycol) hydrogels. The rate of protein diffusion decreased with increasing molecular weight. A computational model of protein diffusion through capillary tubes was also created to predict and verify experimental protein diffusion coefficients. This in vitro capillary tube-based method of measuring protein diffusion represents a simple strategy to interrogate protein diffusion through natural and synthetic hydrogels and aid in the design of better biomaterial-based delivery vehicles that can effectively modulate protein release. |
format | Online Article Text |
id | pubmed-6324205 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | AIP Publishing LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-63242052019-05-08 A rapid method for determining protein diffusion through hydrogels for regenerative medicine applications Hettiaratchi, Marian H. Schudel, Alex Rouse, Tel García, Andrés J. Thomas, Susan N. Guldberg, Robert E. McDevitt, Todd C. APL Bioeng Articles Hydrogels present versatile platforms for the encapsulation and delivery of proteins and cells for regenerative medicine applications. However, differences in hydrogel cross-linking density, polymer weight content, and affinity for proteins all contribute to diverse diffusion rates of proteins through hydrogel networks. Here, we describe a simple method to accurately measure protein diffusion through hydrogels, within a few hours and without the use of large amounts of protein. We tracked the diffusion of several proteins of varying molecular weights along the axial direction of capillary tubes filled with alginate, collagen, or poly(ethylene glycol) hydrogels. The rate of protein diffusion decreased with increasing molecular weight. A computational model of protein diffusion through capillary tubes was also created to predict and verify experimental protein diffusion coefficients. This in vitro capillary tube-based method of measuring protein diffusion represents a simple strategy to interrogate protein diffusion through natural and synthetic hydrogels and aid in the design of better biomaterial-based delivery vehicles that can effectively modulate protein release. AIP Publishing LLC 2018-06-12 /pmc/articles/PMC6324205/ /pubmed/31069307 http://dx.doi.org/10.1063/1.4999925 Text en © 2018 Author(s). 2473-2877/2018/2(2)/026110/14 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Articles Hettiaratchi, Marian H. Schudel, Alex Rouse, Tel García, Andrés J. Thomas, Susan N. Guldberg, Robert E. McDevitt, Todd C. A rapid method for determining protein diffusion through hydrogels for regenerative medicine applications |
title | A rapid method for determining protein diffusion through hydrogels for regenerative medicine applications |
title_full | A rapid method for determining protein diffusion through hydrogels for regenerative medicine applications |
title_fullStr | A rapid method for determining protein diffusion through hydrogels for regenerative medicine applications |
title_full_unstemmed | A rapid method for determining protein diffusion through hydrogels for regenerative medicine applications |
title_short | A rapid method for determining protein diffusion through hydrogels for regenerative medicine applications |
title_sort | rapid method for determining protein diffusion through hydrogels for regenerative medicine applications |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324205/ https://www.ncbi.nlm.nih.gov/pubmed/31069307 http://dx.doi.org/10.1063/1.4999925 |
work_keys_str_mv | AT hettiaratchimarianh arapidmethodfordeterminingproteindiffusionthroughhydrogelsforregenerativemedicineapplications AT schudelalex arapidmethodfordeterminingproteindiffusionthroughhydrogelsforregenerativemedicineapplications AT rousetel arapidmethodfordeterminingproteindiffusionthroughhydrogelsforregenerativemedicineapplications AT garciaandresj arapidmethodfordeterminingproteindiffusionthroughhydrogelsforregenerativemedicineapplications AT thomassusann arapidmethodfordeterminingproteindiffusionthroughhydrogelsforregenerativemedicineapplications AT guldbergroberte arapidmethodfordeterminingproteindiffusionthroughhydrogelsforregenerativemedicineapplications AT mcdevitttoddc arapidmethodfordeterminingproteindiffusionthroughhydrogelsforregenerativemedicineapplications AT hettiaratchimarianh rapidmethodfordeterminingproteindiffusionthroughhydrogelsforregenerativemedicineapplications AT schudelalex rapidmethodfordeterminingproteindiffusionthroughhydrogelsforregenerativemedicineapplications AT rousetel rapidmethodfordeterminingproteindiffusionthroughhydrogelsforregenerativemedicineapplications AT garciaandresj rapidmethodfordeterminingproteindiffusionthroughhydrogelsforregenerativemedicineapplications AT thomassusann rapidmethodfordeterminingproteindiffusionthroughhydrogelsforregenerativemedicineapplications AT guldbergroberte rapidmethodfordeterminingproteindiffusionthroughhydrogelsforregenerativemedicineapplications AT mcdevitttoddc rapidmethodfordeterminingproteindiffusionthroughhydrogelsforregenerativemedicineapplications |