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Anomalously diffusing and persistently migrating cells in 2D and 3D culture environments
Appropriately chosen descriptive models of cell migration in biomaterials will allow researchers to characterize and ultimately predict the movement of cells in engineered systems for a variety of applications in tissue engineering. The persistent random walk (PRW) model accurately describes cell mi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIP Publishing LLC
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324209/ https://www.ncbi.nlm.nih.gov/pubmed/31069309 http://dx.doi.org/10.1063/1.5019196 |
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author | Luzhansky, Igor D. Schwartz, Alyssa D. Cohen, Joshua D. MacMunn, John P. Barney, Lauren E. Jansen, Lauren E. Peyton, Shelly R. |
author_facet | Luzhansky, Igor D. Schwartz, Alyssa D. Cohen, Joshua D. MacMunn, John P. Barney, Lauren E. Jansen, Lauren E. Peyton, Shelly R. |
author_sort | Luzhansky, Igor D. |
collection | PubMed |
description | Appropriately chosen descriptive models of cell migration in biomaterials will allow researchers to characterize and ultimately predict the movement of cells in engineered systems for a variety of applications in tissue engineering. The persistent random walk (PRW) model accurately describes cell migration on two-dimensional (2D) substrates. However, this model inherently cannot describe subdiffusive cell movement, i.e., migration paths in which the root mean square displacement increases more slowly than the square root of the time interval. Subdiffusivity is a common characteristic of cells moving in confined environments, such as three-dimensional (3D) porous scaffolds, hydrogel networks, and in vivo tissues. We demonstrate that a generalized anomalous diffusion (AD) model, which uses a simple power law to relate the mean square displacement to time, more accurately captures individual cell migration paths across a range of engineered 2D and 3D environments than does the more commonly used PRW model. We used the AD model parameters to distinguish cell movement profiles on substrates with different chemokinetic factors, geometries (2D vs 3D), substrate adhesivities, and compliances. Although the two models performed with equal precision for superdiffusive cells, we suggest a simple AD model, in lieu of PRW, to describe cell trajectories in populations with a significant subdiffusive fraction, such as cells in confined, 3D environments. |
format | Online Article Text |
id | pubmed-6324209 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | AIP Publishing LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-63242092019-05-08 Anomalously diffusing and persistently migrating cells in 2D and 3D culture environments Luzhansky, Igor D. Schwartz, Alyssa D. Cohen, Joshua D. MacMunn, John P. Barney, Lauren E. Jansen, Lauren E. Peyton, Shelly R. APL Bioeng Articles Appropriately chosen descriptive models of cell migration in biomaterials will allow researchers to characterize and ultimately predict the movement of cells in engineered systems for a variety of applications in tissue engineering. The persistent random walk (PRW) model accurately describes cell migration on two-dimensional (2D) substrates. However, this model inherently cannot describe subdiffusive cell movement, i.e., migration paths in which the root mean square displacement increases more slowly than the square root of the time interval. Subdiffusivity is a common characteristic of cells moving in confined environments, such as three-dimensional (3D) porous scaffolds, hydrogel networks, and in vivo tissues. We demonstrate that a generalized anomalous diffusion (AD) model, which uses a simple power law to relate the mean square displacement to time, more accurately captures individual cell migration paths across a range of engineered 2D and 3D environments than does the more commonly used PRW model. We used the AD model parameters to distinguish cell movement profiles on substrates with different chemokinetic factors, geometries (2D vs 3D), substrate adhesivities, and compliances. Although the two models performed with equal precision for superdiffusive cells, we suggest a simple AD model, in lieu of PRW, to describe cell trajectories in populations with a significant subdiffusive fraction, such as cells in confined, 3D environments. AIP Publishing LLC 2018-06-19 /pmc/articles/PMC6324209/ /pubmed/31069309 http://dx.doi.org/10.1063/1.5019196 Text en © 2018 Author(s). 2473-2877/2018/2(2)/026112/15 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Articles Luzhansky, Igor D. Schwartz, Alyssa D. Cohen, Joshua D. MacMunn, John P. Barney, Lauren E. Jansen, Lauren E. Peyton, Shelly R. Anomalously diffusing and persistently migrating cells in 2D and 3D culture environments |
title | Anomalously diffusing and persistently migrating cells in 2D and 3D culture environments |
title_full | Anomalously diffusing and persistently migrating cells in 2D and 3D culture environments |
title_fullStr | Anomalously diffusing and persistently migrating cells in 2D and 3D culture environments |
title_full_unstemmed | Anomalously diffusing and persistently migrating cells in 2D and 3D culture environments |
title_short | Anomalously diffusing and persistently migrating cells in 2D and 3D culture environments |
title_sort | anomalously diffusing and persistently migrating cells in 2d and 3d culture environments |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324209/ https://www.ncbi.nlm.nih.gov/pubmed/31069309 http://dx.doi.org/10.1063/1.5019196 |
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