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Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs
This study aimed to evaluate neuropeptide expression after bleaching treatment using histopathological and immunohistochemical analyses and the effects of hydrocortisone and acetaminophen on pulp inflammation, sine dental bleaching and inflammation first occur, and only then, the treatmentt. Sixty-t...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324791/ https://www.ncbi.nlm.nih.gov/pubmed/30620760 http://dx.doi.org/10.1371/journal.pone.0210338 |
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author | Gallinari, Marjorie de Oliveira Cintra, Luciano Tavares Ângelo Benetti, Francine Rahal, Vanessa Ervolino, Edilson Briso, André Luiz Fraga |
author_facet | Gallinari, Marjorie de Oliveira Cintra, Luciano Tavares Ângelo Benetti, Francine Rahal, Vanessa Ervolino, Edilson Briso, André Luiz Fraga |
author_sort | Gallinari, Marjorie de Oliveira |
collection | PubMed |
description | This study aimed to evaluate neuropeptide expression after bleaching treatment using histopathological and immunohistochemical analyses and the effects of hydrocortisone and acetaminophen on pulp inflammation, sine dental bleaching and inflammation first occur, and only then, the treatmentt. Sixty-three rats were divided into three groups (n = 21) according to the pain-relieving therapy used: I-control; II-topical application of Otosporin for 10 min after the bleaching treatment; III-oral administration of paracetamol 30 min before whitening and then every 12h. In all the study groups, placebo gel was applied to the left upper jaw (control) and a 35% H(2)O(2)-based whitening gel was applied to the right upper jaw for 45 min. Seven animals from each group were euthanized at different time points: 0h after treatment, 24h, and 48h. After euthanasia, the first molar on each side was analyzed by histology and immunohistochemistry to assess the degree of inflammation and verify the presence of the neuropeptides, substance P (SP) and calcitonin gene-related peptide (CGRP). The data were analyzed using the statistical nonparametric Kruskal-Wallis test followed by Dunn's test for individual comparisons. Extensive areas of necrosis were observed in the groups that received bleaching treatment only, whereas reduced damage were obtained in the group treated with Otosporin. The immunohistochemical analysis showed positive immunolabeling in all groups, including the control, but this was stronger in the groups that received bleaching treatment. The best results were obtained in the group that received treatment with Otosporin. The use of Otosporin after dental bleaching minimized the side effects of this treatment. |
format | Online Article Text |
id | pubmed-6324791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-63247912019-01-19 Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs Gallinari, Marjorie de Oliveira Cintra, Luciano Tavares Ângelo Benetti, Francine Rahal, Vanessa Ervolino, Edilson Briso, André Luiz Fraga PLoS One Research Article This study aimed to evaluate neuropeptide expression after bleaching treatment using histopathological and immunohistochemical analyses and the effects of hydrocortisone and acetaminophen on pulp inflammation, sine dental bleaching and inflammation first occur, and only then, the treatmentt. Sixty-three rats were divided into three groups (n = 21) according to the pain-relieving therapy used: I-control; II-topical application of Otosporin for 10 min after the bleaching treatment; III-oral administration of paracetamol 30 min before whitening and then every 12h. In all the study groups, placebo gel was applied to the left upper jaw (control) and a 35% H(2)O(2)-based whitening gel was applied to the right upper jaw for 45 min. Seven animals from each group were euthanized at different time points: 0h after treatment, 24h, and 48h. After euthanasia, the first molar on each side was analyzed by histology and immunohistochemistry to assess the degree of inflammation and verify the presence of the neuropeptides, substance P (SP) and calcitonin gene-related peptide (CGRP). The data were analyzed using the statistical nonparametric Kruskal-Wallis test followed by Dunn's test for individual comparisons. Extensive areas of necrosis were observed in the groups that received bleaching treatment only, whereas reduced damage were obtained in the group treated with Otosporin. The immunohistochemical analysis showed positive immunolabeling in all groups, including the control, but this was stronger in the groups that received bleaching treatment. The best results were obtained in the group that received treatment with Otosporin. The use of Otosporin after dental bleaching minimized the side effects of this treatment. Public Library of Science 2019-01-08 /pmc/articles/PMC6324791/ /pubmed/30620760 http://dx.doi.org/10.1371/journal.pone.0210338 Text en © 2019 Gallinari et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Gallinari, Marjorie de Oliveira Cintra, Luciano Tavares Ângelo Benetti, Francine Rahal, Vanessa Ervolino, Edilson Briso, André Luiz Fraga Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs |
title | Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs |
title_full | Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs |
title_fullStr | Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs |
title_full_unstemmed | Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs |
title_short | Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs |
title_sort | pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324791/ https://www.ncbi.nlm.nih.gov/pubmed/30620760 http://dx.doi.org/10.1371/journal.pone.0210338 |
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