Cargando…
Evoked transients of pH-sensitive fluorescent false neurotransmitter reveal dopamine hot spots in the globus pallidus
Dopamine neurotransmission is suspected to play important physiological roles in multiple sparsely innervated brain nuclei, but there has not been a means to measure synaptic dopamine release in such regions. The globus pallidus externa (GPe) is a major locus in the basal ganglia that displays a spa...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324876/ https://www.ncbi.nlm.nih.gov/pubmed/30566076 http://dx.doi.org/10.7554/eLife.42383 |
_version_ | 1783386047100485632 |
---|---|
author | Meszaros, Jozsef Cheung, Timothy Erler, Maya M Kang, Un Jung Sames, Dalibor Kellendonk, Christoph Sulzer, David |
author_facet | Meszaros, Jozsef Cheung, Timothy Erler, Maya M Kang, Un Jung Sames, Dalibor Kellendonk, Christoph Sulzer, David |
author_sort | Meszaros, Jozsef |
collection | PubMed |
description | Dopamine neurotransmission is suspected to play important physiological roles in multiple sparsely innervated brain nuclei, but there has not been a means to measure synaptic dopamine release in such regions. The globus pallidus externa (GPe) is a major locus in the basal ganglia that displays a sparse innervation of en passant dopamine axonal fibers. Due to the low levels of innervation that preclude electrochemical analysis, it is unknown if these axons engage in neurotransmission. To address this, we introduce an optical approach using a pH-sensitive fluorescent false neurotransmitter, FFN102, that exhibits increased fluorescence upon exocytosis from the acidic synaptic vesicle to the neutral extracellular milieu. In marked contrast to the striatum, FFN102 transients in the mouse GPe were spatially heterogeneous and smaller than in striatum with the exception of sparse hot spots. GPe transients were also significantly enhanced by high frequency stimulation. Our results support hot spots of dopamine release from substantia nigra axons. |
format | Online Article Text |
id | pubmed-6324876 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-63248762019-01-10 Evoked transients of pH-sensitive fluorescent false neurotransmitter reveal dopamine hot spots in the globus pallidus Meszaros, Jozsef Cheung, Timothy Erler, Maya M Kang, Un Jung Sames, Dalibor Kellendonk, Christoph Sulzer, David eLife Neuroscience Dopamine neurotransmission is suspected to play important physiological roles in multiple sparsely innervated brain nuclei, but there has not been a means to measure synaptic dopamine release in such regions. The globus pallidus externa (GPe) is a major locus in the basal ganglia that displays a sparse innervation of en passant dopamine axonal fibers. Due to the low levels of innervation that preclude electrochemical analysis, it is unknown if these axons engage in neurotransmission. To address this, we introduce an optical approach using a pH-sensitive fluorescent false neurotransmitter, FFN102, that exhibits increased fluorescence upon exocytosis from the acidic synaptic vesicle to the neutral extracellular milieu. In marked contrast to the striatum, FFN102 transients in the mouse GPe were spatially heterogeneous and smaller than in striatum with the exception of sparse hot spots. GPe transients were also significantly enhanced by high frequency stimulation. Our results support hot spots of dopamine release from substantia nigra axons. eLife Sciences Publications, Ltd 2018-12-19 /pmc/articles/PMC6324876/ /pubmed/30566076 http://dx.doi.org/10.7554/eLife.42383 Text en © 2018, Meszaros et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Meszaros, Jozsef Cheung, Timothy Erler, Maya M Kang, Un Jung Sames, Dalibor Kellendonk, Christoph Sulzer, David Evoked transients of pH-sensitive fluorescent false neurotransmitter reveal dopamine hot spots in the globus pallidus |
title | Evoked transients of pH-sensitive fluorescent false neurotransmitter reveal dopamine hot spots in the globus pallidus |
title_full | Evoked transients of pH-sensitive fluorescent false neurotransmitter reveal dopamine hot spots in the globus pallidus |
title_fullStr | Evoked transients of pH-sensitive fluorescent false neurotransmitter reveal dopamine hot spots in the globus pallidus |
title_full_unstemmed | Evoked transients of pH-sensitive fluorescent false neurotransmitter reveal dopamine hot spots in the globus pallidus |
title_short | Evoked transients of pH-sensitive fluorescent false neurotransmitter reveal dopamine hot spots in the globus pallidus |
title_sort | evoked transients of ph-sensitive fluorescent false neurotransmitter reveal dopamine hot spots in the globus pallidus |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324876/ https://www.ncbi.nlm.nih.gov/pubmed/30566076 http://dx.doi.org/10.7554/eLife.42383 |
work_keys_str_mv | AT meszarosjozsef evokedtransientsofphsensitivefluorescentfalseneurotransmitterrevealdopaminehotspotsintheglobuspallidus AT cheungtimothy evokedtransientsofphsensitivefluorescentfalseneurotransmitterrevealdopaminehotspotsintheglobuspallidus AT erlermayam evokedtransientsofphsensitivefluorescentfalseneurotransmitterrevealdopaminehotspotsintheglobuspallidus AT kangunjung evokedtransientsofphsensitivefluorescentfalseneurotransmitterrevealdopaminehotspotsintheglobuspallidus AT samesdalibor evokedtransientsofphsensitivefluorescentfalseneurotransmitterrevealdopaminehotspotsintheglobuspallidus AT kellendonkchristoph evokedtransientsofphsensitivefluorescentfalseneurotransmitterrevealdopaminehotspotsintheglobuspallidus AT sulzerdavid evokedtransientsofphsensitivefluorescentfalseneurotransmitterrevealdopaminehotspotsintheglobuspallidus |