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Effects of zinc-bearing palygorskite on rumen fermentation in vitro

OBJECTIVE: The aim of the study was to investigate the effect of zinc-bearing palygorskite (Zn-Pal) on rumen fermentation by in vitro gas-production system. METHODS: In trial, 90 incubators were evenly divided into five groups: control (0% Zn-Pal), treatment I (0.2% Zn-Pal), treatment II (0.4% Zn-Pa...

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Autores principales: Chen, Mengjiao, Xi, Yumeng, Zhang, Lin, Zeng, Hanfang, Li, Yeqing, Han, Zhaoyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325408/
https://www.ncbi.nlm.nih.gov/pubmed/29747497
http://dx.doi.org/10.5713/ajas.17.0920
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author Chen, Mengjiao
Xi, Yumeng
Zhang, Lin
Zeng, Hanfang
Li, Yeqing
Han, Zhaoyu
author_facet Chen, Mengjiao
Xi, Yumeng
Zhang, Lin
Zeng, Hanfang
Li, Yeqing
Han, Zhaoyu
author_sort Chen, Mengjiao
collection PubMed
description OBJECTIVE: The aim of the study was to investigate the effect of zinc-bearing palygorskite (Zn-Pal) on rumen fermentation by in vitro gas-production system. METHODS: In trial, 90 incubators were evenly divided into five groups: control (0% Zn-Pal), treatment I (0.2% Zn-Pal), treatment II (0.4% Zn-Pal), treatment III (0.6% Zn-Pal), and treatment IV (0.8% Zn-Pal). The contents of zinc for treatments were 0, 49, 98, 147, 196 mg/kg, respectively. The main chemical composition and microstructure of Zn-Pal was investigated by X-ray diffraction. The physicochemical features were evaluated by Zeta potential analysis, cation-exchange capacity, ethylene blue absorption and specific surface area (the Brunauer–Emmett–Teller method). In vitro gas production (GP) was recorded at 3, 6, 9, 12, 18, 24, 36, 48, 60, and 72 h incubation. Incubation was stopped at 0, 6, 12, 24, 48, and 72 h and the inoculants were tested for pH, microbial protein yield (MCP), NH(3)-N, volatile fatty acids (VFAs), lipopolysaccharide (LPS). RESULTS: The results showed that the GP in the treatment groups was not significantly different from the control groups (p>0.05). Compared to the control group, pH was higher at 24 h, 48 h (p<0.05), and 72 h (p<0.01) (range 6 to 7). The concentration of NH(3)-N in the three treatment groups was higher than in the control group at 24 h (p<0.01), meanwhile, it was lower at 48 h and 72 h (p<0.01), except in the treatment IV. The concentration of MCP in treatment I group was higher than in the control at 48 h (p<0.01). Compared with control, the LPS concentration in treatment III became lower at 12 h (p<0.05). Total VFAs in treatments were higher than in the control at 24 h, 48 h (p<0.05). CONCLUSION: These results suggest that the addition of Zn-Pal can improve the rumen fermentation, especially when adding 0.2% Zn-Pal.
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spelling pubmed-63254082019-01-17 Effects of zinc-bearing palygorskite on rumen fermentation in vitro Chen, Mengjiao Xi, Yumeng Zhang, Lin Zeng, Hanfang Li, Yeqing Han, Zhaoyu Asian-Australas J Anim Sci Article OBJECTIVE: The aim of the study was to investigate the effect of zinc-bearing palygorskite (Zn-Pal) on rumen fermentation by in vitro gas-production system. METHODS: In trial, 90 incubators were evenly divided into five groups: control (0% Zn-Pal), treatment I (0.2% Zn-Pal), treatment II (0.4% Zn-Pal), treatment III (0.6% Zn-Pal), and treatment IV (0.8% Zn-Pal). The contents of zinc for treatments were 0, 49, 98, 147, 196 mg/kg, respectively. The main chemical composition and microstructure of Zn-Pal was investigated by X-ray diffraction. The physicochemical features were evaluated by Zeta potential analysis, cation-exchange capacity, ethylene blue absorption and specific surface area (the Brunauer–Emmett–Teller method). In vitro gas production (GP) was recorded at 3, 6, 9, 12, 18, 24, 36, 48, 60, and 72 h incubation. Incubation was stopped at 0, 6, 12, 24, 48, and 72 h and the inoculants were tested for pH, microbial protein yield (MCP), NH(3)-N, volatile fatty acids (VFAs), lipopolysaccharide (LPS). RESULTS: The results showed that the GP in the treatment groups was not significantly different from the control groups (p>0.05). Compared to the control group, pH was higher at 24 h, 48 h (p<0.05), and 72 h (p<0.01) (range 6 to 7). The concentration of NH(3)-N in the three treatment groups was higher than in the control group at 24 h (p<0.01), meanwhile, it was lower at 48 h and 72 h (p<0.01), except in the treatment IV. The concentration of MCP in treatment I group was higher than in the control at 48 h (p<0.01). Compared with control, the LPS concentration in treatment III became lower at 12 h (p<0.05). Total VFAs in treatments were higher than in the control at 24 h, 48 h (p<0.05). CONCLUSION: These results suggest that the addition of Zn-Pal can improve the rumen fermentation, especially when adding 0.2% Zn-Pal. Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2019-01 2018-04-19 /pmc/articles/PMC6325408/ /pubmed/29747497 http://dx.doi.org/10.5713/ajas.17.0920 Text en Copyright © 2019 by Asian-Australasian Journal of Animal Sciences This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Chen, Mengjiao
Xi, Yumeng
Zhang, Lin
Zeng, Hanfang
Li, Yeqing
Han, Zhaoyu
Effects of zinc-bearing palygorskite on rumen fermentation in vitro
title Effects of zinc-bearing palygorskite on rumen fermentation in vitro
title_full Effects of zinc-bearing palygorskite on rumen fermentation in vitro
title_fullStr Effects of zinc-bearing palygorskite on rumen fermentation in vitro
title_full_unstemmed Effects of zinc-bearing palygorskite on rumen fermentation in vitro
title_short Effects of zinc-bearing palygorskite on rumen fermentation in vitro
title_sort effects of zinc-bearing palygorskite on rumen fermentation in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325408/
https://www.ncbi.nlm.nih.gov/pubmed/29747497
http://dx.doi.org/10.5713/ajas.17.0920
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