Chloride promotes refolding of active Vibrio alkaline phosphatase through an inactive dimeric intermediate with an altered interface

Most enzymes are homodimers or higher order multimers. Cold‐active alkaline phosphatase from Vibrio splendidus (VAP) transitions into a dimer with very low activity under mild denaturation conditions. The desire to understand why this dimer fails to efficiently catalyse phosphomonoester hydrolysis l...

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Detalles Bibliográficos
Autores principales: Hjörleifsson, Jens Guðmundur, Ásgeirsson, Bjarni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325577/
https://www.ncbi.nlm.nih.gov/pubmed/30652084
http://dx.doi.org/10.1002/2211-5463.12565

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325577/
https://www.ncbi.nlm.nih.gov/pubmed/30652084
http://dx.doi.org/10.1002/2211-5463.12565

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