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Expression cloning human and rat renal cortex Na/P(i) cotransporters: behind the scenes in the Murer laboratory

In the pre-genomic era, the cloning of a cDNA represented a significant achievement, particularly if the gene of interest encoded a membrane protein. At the time, molecular probes such as partial peptide sequences, suitable nucleic acid sequences, or antibodies were unavailable for most proteins and...

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Detalles Bibliográficos
Autores principales: Magagnin, Simona, Werner, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326002/
https://www.ncbi.nlm.nih.gov/pubmed/30175377
http://dx.doi.org/10.1007/s00424-018-2198-9
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author Magagnin, Simona
Werner, Andreas
author_facet Magagnin, Simona
Werner, Andreas
author_sort Magagnin, Simona
collection PubMed
description In the pre-genomic era, the cloning of a cDNA represented a significant achievement, particularly if the gene of interest encoded a membrane protein. At the time, molecular probes such as partial peptide sequences, suitable nucleic acid sequences, or antibodies were unavailable for most proteins and the “sodium-phosphate transporter” was no exception. In contrast, brush-border membrane vesicles and epithelial cell culture experiments had established a reliable set of functional hallmarks that described Na-dependent phosphate transport activity in some detail. Moreover, aspects of hormonal regulation of phosphate homeostasis could be recapitulated in these model systems. Expression cloning elegantly combined functional protein expression in Xenopus laevis oocytes with molecular biology to overcome the lack of molecular probes.
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spelling pubmed-63260022019-01-23 Expression cloning human and rat renal cortex Na/P(i) cotransporters: behind the scenes in the Murer laboratory Magagnin, Simona Werner, Andreas Pflugers Arch Invited Review In the pre-genomic era, the cloning of a cDNA represented a significant achievement, particularly if the gene of interest encoded a membrane protein. At the time, molecular probes such as partial peptide sequences, suitable nucleic acid sequences, or antibodies were unavailable for most proteins and the “sodium-phosphate transporter” was no exception. In contrast, brush-border membrane vesicles and epithelial cell culture experiments had established a reliable set of functional hallmarks that described Na-dependent phosphate transport activity in some detail. Moreover, aspects of hormonal regulation of phosphate homeostasis could be recapitulated in these model systems. Expression cloning elegantly combined functional protein expression in Xenopus laevis oocytes with molecular biology to overcome the lack of molecular probes. Springer Berlin Heidelberg 2018-09-03 2019 /pmc/articles/PMC6326002/ /pubmed/30175377 http://dx.doi.org/10.1007/s00424-018-2198-9 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Invited Review
Magagnin, Simona
Werner, Andreas
Expression cloning human and rat renal cortex Na/P(i) cotransporters: behind the scenes in the Murer laboratory
title Expression cloning human and rat renal cortex Na/P(i) cotransporters: behind the scenes in the Murer laboratory
title_full Expression cloning human and rat renal cortex Na/P(i) cotransporters: behind the scenes in the Murer laboratory
title_fullStr Expression cloning human and rat renal cortex Na/P(i) cotransporters: behind the scenes in the Murer laboratory
title_full_unstemmed Expression cloning human and rat renal cortex Na/P(i) cotransporters: behind the scenes in the Murer laboratory
title_short Expression cloning human and rat renal cortex Na/P(i) cotransporters: behind the scenes in the Murer laboratory
title_sort expression cloning human and rat renal cortex na/p(i) cotransporters: behind the scenes in the murer laboratory
topic Invited Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326002/
https://www.ncbi.nlm.nih.gov/pubmed/30175377
http://dx.doi.org/10.1007/s00424-018-2198-9
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