The sequence and de novo assembly of hog deer genome

Hog deer (Axis porcinus) is a small deer species in family Cervidae and has been undergoing a serious and global decline during the past decades. Chengdu Zoo currently holds a captive population of hog deer with sufficient genetic diversity in China. We sequenced and de novo assembled its genome seq...

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Detalles Bibliográficos
Autores principales: Wang, Wei, Yan, Hui-Juan, Chen, Shi-Yi, Li, Zhen-Zhen, Yi, Jun, Niu, Li-Li, Deng, Jia-Po, Chen, Wei-Gang, Pu, Yang, Jia, Xianbo, Qu, Yu, Chen, Ang, Zhong, Yan, Yu, Xin-Ming, Pang, Shuai, Huang, Wan-Long, Han, Yue, Liu, Guang-Jian, Yu, Jian-Qiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2019
Materias:
Data Descriptor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326164/
https://www.ncbi.nlm.nih.gov/pubmed/30620341
http://dx.doi.org/10.1038/sdata.2018.305
Descripción
Sumario:Hog deer (Axis porcinus) is a small deer species in family Cervidae and has been undergoing a serious and global decline during the past decades. Chengdu Zoo currently holds a captive population of hog deer with sufficient genetic diversity in China. We sequenced and de novo assembled its genome sequence in the present study. A total of six different insert-size libraries were sequenced and generated 395 Gb of clean data in total. With aid of the linked reads of 10X Genomics, genome sequence was assembled to 2.72 Gb in length (contig N50, 66.04 Kb; scaffold N50, 20.55 Mb), in which 94.5% of expected genes were detected. We comprehensively annotated 22,473 protein-coding genes, 37,019 tRNAs, and 1,058 Mb repeated sequences. The newly generated reference genome is expected to significantly contribute to comparative analysis of genome biology and evolution within family Cervidae.

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