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The sequence and de novo assembly of hog deer genome
Hog deer (Axis porcinus) is a small deer species in family Cervidae and has been undergoing a serious and global decline during the past decades. Chengdu Zoo currently holds a captive population of hog deer with sufficient genetic diversity in China. We sequenced and de novo assembled its genome seq...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326164/ https://www.ncbi.nlm.nih.gov/pubmed/30620341 http://dx.doi.org/10.1038/sdata.2018.305 |
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author | Wang, Wei Yan, Hui-Juan Chen, Shi-Yi Li, Zhen-Zhen Yi, Jun Niu, Li-Li Deng, Jia-Po Chen, Wei-Gang Pu, Yang Jia, Xianbo Qu, Yu Chen, Ang Zhong, Yan Yu, Xin-Ming Pang, Shuai Huang, Wan-Long Han, Yue Liu, Guang-Jian Yu, Jian-Qiu |
author_facet | Wang, Wei Yan, Hui-Juan Chen, Shi-Yi Li, Zhen-Zhen Yi, Jun Niu, Li-Li Deng, Jia-Po Chen, Wei-Gang Pu, Yang Jia, Xianbo Qu, Yu Chen, Ang Zhong, Yan Yu, Xin-Ming Pang, Shuai Huang, Wan-Long Han, Yue Liu, Guang-Jian Yu, Jian-Qiu |
author_sort | Wang, Wei |
collection | PubMed |
description | Hog deer (Axis porcinus) is a small deer species in family Cervidae and has been undergoing a serious and global decline during the past decades. Chengdu Zoo currently holds a captive population of hog deer with sufficient genetic diversity in China. We sequenced and de novo assembled its genome sequence in the present study. A total of six different insert-size libraries were sequenced and generated 395 Gb of clean data in total. With aid of the linked reads of 10X Genomics, genome sequence was assembled to 2.72 Gb in length (contig N50, 66.04 Kb; scaffold N50, 20.55 Mb), in which 94.5% of expected genes were detected. We comprehensively annotated 22,473 protein-coding genes, 37,019 tRNAs, and 1,058 Mb repeated sequences. The newly generated reference genome is expected to significantly contribute to comparative analysis of genome biology and evolution within family Cervidae. |
format | Online Article Text |
id | pubmed-6326164 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-63261642019-01-10 The sequence and de novo assembly of hog deer genome Wang, Wei Yan, Hui-Juan Chen, Shi-Yi Li, Zhen-Zhen Yi, Jun Niu, Li-Li Deng, Jia-Po Chen, Wei-Gang Pu, Yang Jia, Xianbo Qu, Yu Chen, Ang Zhong, Yan Yu, Xin-Ming Pang, Shuai Huang, Wan-Long Han, Yue Liu, Guang-Jian Yu, Jian-Qiu Sci Data Data Descriptor Hog deer (Axis porcinus) is a small deer species in family Cervidae and has been undergoing a serious and global decline during the past decades. Chengdu Zoo currently holds a captive population of hog deer with sufficient genetic diversity in China. We sequenced and de novo assembled its genome sequence in the present study. A total of six different insert-size libraries were sequenced and generated 395 Gb of clean data in total. With aid of the linked reads of 10X Genomics, genome sequence was assembled to 2.72 Gb in length (contig N50, 66.04 Kb; scaffold N50, 20.55 Mb), in which 94.5% of expected genes were detected. We comprehensively annotated 22,473 protein-coding genes, 37,019 tRNAs, and 1,058 Mb repeated sequences. The newly generated reference genome is expected to significantly contribute to comparative analysis of genome biology and evolution within family Cervidae. Nature Publishing Group 2019-01-08 /pmc/articles/PMC6326164/ /pubmed/30620341 http://dx.doi.org/10.1038/sdata.2018.305 Text en Copyright © 2019, The Author(s) http://creativecommons.org/licenses/by/4.0/ Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files made available in this article. |
spellingShingle | Data Descriptor Wang, Wei Yan, Hui-Juan Chen, Shi-Yi Li, Zhen-Zhen Yi, Jun Niu, Li-Li Deng, Jia-Po Chen, Wei-Gang Pu, Yang Jia, Xianbo Qu, Yu Chen, Ang Zhong, Yan Yu, Xin-Ming Pang, Shuai Huang, Wan-Long Han, Yue Liu, Guang-Jian Yu, Jian-Qiu The sequence and de novo assembly of hog deer genome |
title | The sequence and de novo assembly of hog deer genome |
title_full | The sequence and de novo assembly of hog deer genome |
title_fullStr | The sequence and de novo assembly of hog deer genome |
title_full_unstemmed | The sequence and de novo assembly of hog deer genome |
title_short | The sequence and de novo assembly of hog deer genome |
title_sort | sequence and de novo assembly of hog deer genome |
topic | Data Descriptor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326164/ https://www.ncbi.nlm.nih.gov/pubmed/30620341 http://dx.doi.org/10.1038/sdata.2018.305 |
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