A protocol for generating induced T cells by reprogramming B cells in vivo

Obtaining T cells by reprogramming is one of the major goals in regenerative medicine. Here, we describe a protocol for generating functional T cells from Hoxb5-expressing pro/pre-B cells in vivo. This protocol includes the construction of Hoxb5 recombinant plasmids, retroviral packaging, isolation...

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Detalles Bibliográficos
Autores principales: Weng, Qitong, Hu, Fangxiao, Zhang, Mengyun, Dong, Yong, Lv, Cui, Wang, Ying, Liu, Xiaofei, Wang, Jinyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chinese Society for Cell Biology 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326249/
https://www.ncbi.nlm.nih.gov/pubmed/30671224
http://dx.doi.org/10.1016/j.cr.2018.05.001
Descripción
Sumario:Obtaining T cells by reprogramming is one of the major goals in regenerative medicine. Here, we describe a protocol for generating functional T cells from Hoxb5-expressing pro/pre-B cells in vivo. This protocol includes the construction of Hoxb5 recombinant plasmids, retroviral packaging, isolation and viral transduction of pro/pre-B cells, cell transplantation, and phenotypic analysis of induced T cells. The procedure is reproducible and straightforward, providing an approach for generating induced T cells for translational research.

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