A protocol for generating induced T cells by reprogramming B cells in vivo

Obtaining T cells by reprogramming is one of the major goals in regenerative medicine. Here, we describe a protocol for generating functional T cells from Hoxb5-expressing pro/pre-B cells in vivo. This protocol includes the construction of Hoxb5 recombinant plasmids, retroviral packaging, isolation...

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Autores principales: Weng, Qitong, Hu, Fangxiao, Zhang, Mengyun, Dong, Yong, Lv, Cui, Wang, Ying, Liu, Xiaofei, Wang, Jinyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chinese Society for Cell Biology 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326249/
https://www.ncbi.nlm.nih.gov/pubmed/30671224
http://dx.doi.org/10.1016/j.cr.2018.05.001
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author Weng, Qitong
Hu, Fangxiao
Zhang, Mengyun
Dong, Yong
Lv, Cui
Wang, Ying
Liu, Xiaofei
Wang, Jinyong
author_facet Weng, Qitong
Hu, Fangxiao
Zhang, Mengyun
Dong, Yong
Lv, Cui
Wang, Ying
Liu, Xiaofei
Wang, Jinyong
author_sort Weng, Qitong
collection PubMed
description Obtaining T cells by reprogramming is one of the major goals in regenerative medicine. Here, we describe a protocol for generating functional T cells from Hoxb5-expressing pro/pre-B cells in vivo. This protocol includes the construction of Hoxb5 recombinant plasmids, retroviral packaging, isolation and viral transduction of pro/pre-B cells, cell transplantation, and phenotypic analysis of induced T cells. The procedure is reproducible and straightforward, providing an approach for generating induced T cells for translational research.
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spelling pubmed-63262492019-01-22 A protocol for generating induced T cells by reprogramming B cells in vivo Weng, Qitong Hu, Fangxiao Zhang, Mengyun Dong, Yong Lv, Cui Wang, Ying Liu, Xiaofei Wang, Jinyong Cell Regen Article Obtaining T cells by reprogramming is one of the major goals in regenerative medicine. Here, we describe a protocol for generating functional T cells from Hoxb5-expressing pro/pre-B cells in vivo. This protocol includes the construction of Hoxb5 recombinant plasmids, retroviral packaging, isolation and viral transduction of pro/pre-B cells, cell transplantation, and phenotypic analysis of induced T cells. The procedure is reproducible and straightforward, providing an approach for generating induced T cells for translational research. Chinese Society for Cell Biology 2018-07-19 /pmc/articles/PMC6326249/ /pubmed/30671224 http://dx.doi.org/10.1016/j.cr.2018.05.001 Text en © 2018 Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences. Production and hosting by Elsevier B.V. on behalf of KeAi. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Weng, Qitong
Hu, Fangxiao
Zhang, Mengyun
Dong, Yong
Lv, Cui
Wang, Ying
Liu, Xiaofei
Wang, Jinyong
A protocol for generating induced T cells by reprogramming B cells in vivo
title A protocol for generating induced T cells by reprogramming B cells in vivo
title_full A protocol for generating induced T cells by reprogramming B cells in vivo
title_fullStr A protocol for generating induced T cells by reprogramming B cells in vivo
title_full_unstemmed A protocol for generating induced T cells by reprogramming B cells in vivo
title_short A protocol for generating induced T cells by reprogramming B cells in vivo
title_sort protocol for generating induced t cells by reprogramming b cells in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326249/
https://www.ncbi.nlm.nih.gov/pubmed/30671224
http://dx.doi.org/10.1016/j.cr.2018.05.001
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