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Melatonin alleviates meiotic defects in fetal mouse oocytes induced by Di (2-ethylhexyl) phthalate in vitro

Di (2-ethylhexyl) phthalate (DEHP), an estrogen-like compound that is a ubiquitous environmental contaminant, has been reported to adversely affect human and mammalian reproduction. Many studies have found that exposure to DEHP during pregnancy perturbs female germ cell meiosis and is detrimental to...

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Autores principales: Sun, Zhong-Yi, Zhang, Pan, Wang, Jun-Jie, Liu, Jing-Cai, Li, Lan, Shen, Wei, Zhai, Qiu-Yue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326675/
https://www.ncbi.nlm.nih.gov/pubmed/30591620
http://dx.doi.org/10.18632/aging.101715
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author Sun, Zhong-Yi
Zhang, Pan
Wang, Jun-Jie
Liu, Jing-Cai
Li, Lan
Shen, Wei
Zhai, Qiu-Yue
author_facet Sun, Zhong-Yi
Zhang, Pan
Wang, Jun-Jie
Liu, Jing-Cai
Li, Lan
Shen, Wei
Zhai, Qiu-Yue
author_sort Sun, Zhong-Yi
collection PubMed
description Di (2-ethylhexyl) phthalate (DEHP), an estrogen-like compound that is a ubiquitous environmental contaminant, has been reported to adversely affect human and mammalian reproduction. Many studies have found that exposure to DEHP during pregnancy perturbs female germ cell meiosis and is detrimental to oogenesis. Previous studies have demonstrated that melatonin (MLT) is beneficial to reproductive endocrinology, oogenesis, and embryonic development as the ability to antioxidative and antiapoptotic. However, whether the meiotic defect of germ cells exposed to DEHP could be rescued by MLT is not clear. Here, we cultured 12.5 days post coitum (dpc) fetal mouse ovaries for 6 days, exposed them to 100 μM DEHP with or without 1 μM MLT in vitro.. The results showed that DEHP exposure induced the abnormal formation of DNA double-strand breaks (DSBs), and inhibited the repair of DSBs during meiotic recombination. In addition, we found defective oocytes were prone to undergo apoptosis. Notably, this defect could be remarkably ameliorated by the addition of MLT via a reduction of the levels of reactive oxygen species and an inhibition of apoptosis. In conclusion, our data revealed that MLT had a protective action against the meiotic deterioration of fetal oocytes induced by DEHP in the mouse in vitro.
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spelling pubmed-63266752019-01-16 Melatonin alleviates meiotic defects in fetal mouse oocytes induced by Di (2-ethylhexyl) phthalate in vitro Sun, Zhong-Yi Zhang, Pan Wang, Jun-Jie Liu, Jing-Cai Li, Lan Shen, Wei Zhai, Qiu-Yue Aging (Albany NY) Research Paper Di (2-ethylhexyl) phthalate (DEHP), an estrogen-like compound that is a ubiquitous environmental contaminant, has been reported to adversely affect human and mammalian reproduction. Many studies have found that exposure to DEHP during pregnancy perturbs female germ cell meiosis and is detrimental to oogenesis. Previous studies have demonstrated that melatonin (MLT) is beneficial to reproductive endocrinology, oogenesis, and embryonic development as the ability to antioxidative and antiapoptotic. However, whether the meiotic defect of germ cells exposed to DEHP could be rescued by MLT is not clear. Here, we cultured 12.5 days post coitum (dpc) fetal mouse ovaries for 6 days, exposed them to 100 μM DEHP with or without 1 μM MLT in vitro.. The results showed that DEHP exposure induced the abnormal formation of DNA double-strand breaks (DSBs), and inhibited the repair of DSBs during meiotic recombination. In addition, we found defective oocytes were prone to undergo apoptosis. Notably, this defect could be remarkably ameliorated by the addition of MLT via a reduction of the levels of reactive oxygen species and an inhibition of apoptosis. In conclusion, our data revealed that MLT had a protective action against the meiotic deterioration of fetal oocytes induced by DEHP in the mouse in vitro. Impact Journals 2018-12-26 /pmc/articles/PMC6326675/ /pubmed/30591620 http://dx.doi.org/10.18632/aging.101715 Text en Copyright © 2018 Sun et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Sun, Zhong-Yi
Zhang, Pan
Wang, Jun-Jie
Liu, Jing-Cai
Li, Lan
Shen, Wei
Zhai, Qiu-Yue
Melatonin alleviates meiotic defects in fetal mouse oocytes induced by Di (2-ethylhexyl) phthalate in vitro
title Melatonin alleviates meiotic defects in fetal mouse oocytes induced by Di (2-ethylhexyl) phthalate in vitro
title_full Melatonin alleviates meiotic defects in fetal mouse oocytes induced by Di (2-ethylhexyl) phthalate in vitro
title_fullStr Melatonin alleviates meiotic defects in fetal mouse oocytes induced by Di (2-ethylhexyl) phthalate in vitro
title_full_unstemmed Melatonin alleviates meiotic defects in fetal mouse oocytes induced by Di (2-ethylhexyl) phthalate in vitro
title_short Melatonin alleviates meiotic defects in fetal mouse oocytes induced by Di (2-ethylhexyl) phthalate in vitro
title_sort melatonin alleviates meiotic defects in fetal mouse oocytes induced by di (2-ethylhexyl) phthalate in vitro
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326675/
https://www.ncbi.nlm.nih.gov/pubmed/30591620
http://dx.doi.org/10.18632/aging.101715
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