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Inhibition of protein kinase D disrupts spindle formation and actin assembly during porcine oocyte maturation
Protein kinase D (PKD) subfamily which includes PKD1, PKD2 and PKD3 is a novel family of serine/threonine kinases. PKD has been widely implicated in the regulation of multiple physiological effects including immune responses, apoptosis and cell proliferation. However, the roles of PKD in oocytes hav...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326681/ https://www.ncbi.nlm.nih.gov/pubmed/30555056 http://dx.doi.org/10.18632/aging.101667 |
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author | Zhang, Yu Wang, Hong-Hui Wan, Xiang Xu, Yao Pan, Meng-Hao Sun, Shao-Chen |
author_facet | Zhang, Yu Wang, Hong-Hui Wan, Xiang Xu, Yao Pan, Meng-Hao Sun, Shao-Chen |
author_sort | Zhang, Yu |
collection | PubMed |
description | Protein kinase D (PKD) subfamily which includes PKD1, PKD2 and PKD3 is a novel family of serine/threonine kinases. PKD has been widely implicated in the regulation of multiple physiological effects including immune responses, apoptosis and cell proliferation. However, the roles of PKD in oocytes have not been fully clarified. In this study we investigated the regulatory functions of PKD during porcine oocyte maturation. Our results indicated that PKD expressed in porcine oocytes and the inhibition of PKD family activity led to the failure of meiosis resumption and the first polar body extrusion. Further analysis indicated that the spindle assembly and chromosome alignment were disrupted after PKD family inhibition, and this might be through its regulatory role on MAPK phosphorylation. We also found that PKD phosphorylated cofilin for actin assembly, which further affected cortical actin distribution, indicating the roles of PKD family on cytoskeleton. In addition, a decreased expression of PKD in postovulatory aging porcine oocytes was observed, which might connect PKD with cytoskeleton defects in aged oocytes. Taken together, these results suggest that PKD possesses important functions in porcine oocyte maturation by regulating spindle organization and actin assembly. |
format | Online Article Text |
id | pubmed-6326681 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-63266812019-01-16 Inhibition of protein kinase D disrupts spindle formation and actin assembly during porcine oocyte maturation Zhang, Yu Wang, Hong-Hui Wan, Xiang Xu, Yao Pan, Meng-Hao Sun, Shao-Chen Aging (Albany NY) Research Paper Protein kinase D (PKD) subfamily which includes PKD1, PKD2 and PKD3 is a novel family of serine/threonine kinases. PKD has been widely implicated in the regulation of multiple physiological effects including immune responses, apoptosis and cell proliferation. However, the roles of PKD in oocytes have not been fully clarified. In this study we investigated the regulatory functions of PKD during porcine oocyte maturation. Our results indicated that PKD expressed in porcine oocytes and the inhibition of PKD family activity led to the failure of meiosis resumption and the first polar body extrusion. Further analysis indicated that the spindle assembly and chromosome alignment were disrupted after PKD family inhibition, and this might be through its regulatory role on MAPK phosphorylation. We also found that PKD phosphorylated cofilin for actin assembly, which further affected cortical actin distribution, indicating the roles of PKD family on cytoskeleton. In addition, a decreased expression of PKD in postovulatory aging porcine oocytes was observed, which might connect PKD with cytoskeleton defects in aged oocytes. Taken together, these results suggest that PKD possesses important functions in porcine oocyte maturation by regulating spindle organization and actin assembly. Impact Journals 2018-12-14 /pmc/articles/PMC6326681/ /pubmed/30555056 http://dx.doi.org/10.18632/aging.101667 Text en Copyright © 2018 Zhang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Zhang, Yu Wang, Hong-Hui Wan, Xiang Xu, Yao Pan, Meng-Hao Sun, Shao-Chen Inhibition of protein kinase D disrupts spindle formation and actin assembly during porcine oocyte maturation |
title | Inhibition of protein kinase D disrupts spindle formation and actin assembly during porcine oocyte maturation |
title_full | Inhibition of protein kinase D disrupts spindle formation and actin assembly during porcine oocyte maturation |
title_fullStr | Inhibition of protein kinase D disrupts spindle formation and actin assembly during porcine oocyte maturation |
title_full_unstemmed | Inhibition of protein kinase D disrupts spindle formation and actin assembly during porcine oocyte maturation |
title_short | Inhibition of protein kinase D disrupts spindle formation and actin assembly during porcine oocyte maturation |
title_sort | inhibition of protein kinase d disrupts spindle formation and actin assembly during porcine oocyte maturation |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326681/ https://www.ncbi.nlm.nih.gov/pubmed/30555056 http://dx.doi.org/10.18632/aging.101667 |
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