Data on antigen recognition hindrance by antibodies covalently immobilized to Protein G magnetic beads by dimethyl pimelimidate (DMP) cross-linking

The data presented herein is related to the article entitled "Trypanosoma cruzi immunoproteome: calpain-like CAP5.5 differentially detected throughout distinct stages of human Chagas disease cardiomyopathy" [1]. Electrophoretic analyses under denaturing and reducing conditions indicate tha...

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Detalles Bibliográficos
Autores principales: Caminha, Marcelle A., de Lorena, Virginia Maria B., de Oliveira Júnior, Wilson, Perales, Jonas, Carvalho, Paulo C., Lima, Diogo B., Cavalcanti, Maria da Glória A.M., Martins, Sílvia M., Valente, Richard H., Menna-Barreto, Rubem F.S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Immunology and Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327068/
https://www.ncbi.nlm.nih.gov/pubmed/30671503
http://dx.doi.org/10.1016/j.dib.2018.12.057
Descripción
Sumario:The data presented herein is related to the article entitled "Trypanosoma cruzi immunoproteome: calpain-like CAP5.5 differentially detected throughout distinct stages of human Chagas disease cardiomyopathy" [1]. Electrophoretic analyses under denaturing and reducing conditions indicate that covalent immobilization of human IgG to Protein G magnetic beads by cross-linking with 50 mM dimethyl pimelimidate hinders the recognition of T. cruzi antigens in immunoprecipitation assays.

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