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Molecular Characterization of Hard Ticks by Cytochrome c Oxidase Subunit 1 Sequences

Although widely studied, the natural diversity of the hard tick is not well known. In this study, we collected 194 sequences from 67 species, covering 7 genera of hard tick. The 5′ region of the mitochondrial cytochrome c oxidase subunit 1 region (586 bp) has been used to investigate intra- and inte...

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Autores principales: Gou, Huitian, Xue, Huiwen, Yin, Hong, Luo, Jianxun, Sun, Xiaolin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology and Tropical Medicine 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327197/
https://www.ncbi.nlm.nih.gov/pubmed/30630279
http://dx.doi.org/10.3347/kjp.2018.56.6.583
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author Gou, Huitian
Xue, Huiwen
Yin, Hong
Luo, Jianxun
Sun, Xiaolin
author_facet Gou, Huitian
Xue, Huiwen
Yin, Hong
Luo, Jianxun
Sun, Xiaolin
author_sort Gou, Huitian
collection PubMed
description Although widely studied, the natural diversity of the hard tick is not well known. In this study, we collected 194 sequences from 67 species, covering 7 genera of hard tick. The 5′ region of the mitochondrial cytochrome c oxidase subunit 1 region (586 bp) has been used to investigate intra- and inter-species variation and the phylogenetic tree of neighbor joining method has been used for assessment. As a result, by comparing the K2P-distance of intra- and interspecies, 30 samples (15.2%) shown that interspecies distance was larger than the minimum interspecfic distance. From the phylogenetic analysis, 86.8% (49) of the species were identified correctly at the genus level. On deeper analysis on these species suggested the possibility of presence cryptic species. Therefore, further work is required to delineate species boundaries and to develop a more complete understanding of hard tick diversity over larger scale.
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spelling pubmed-63271972019-01-11 Molecular Characterization of Hard Ticks by Cytochrome c Oxidase Subunit 1 Sequences Gou, Huitian Xue, Huiwen Yin, Hong Luo, Jianxun Sun, Xiaolin Korean J Parasitol Original Article Although widely studied, the natural diversity of the hard tick is not well known. In this study, we collected 194 sequences from 67 species, covering 7 genera of hard tick. The 5′ region of the mitochondrial cytochrome c oxidase subunit 1 region (586 bp) has been used to investigate intra- and inter-species variation and the phylogenetic tree of neighbor joining method has been used for assessment. As a result, by comparing the K2P-distance of intra- and interspecies, 30 samples (15.2%) shown that interspecies distance was larger than the minimum interspecfic distance. From the phylogenetic analysis, 86.8% (49) of the species were identified correctly at the genus level. On deeper analysis on these species suggested the possibility of presence cryptic species. Therefore, further work is required to delineate species boundaries and to develop a more complete understanding of hard tick diversity over larger scale. The Korean Society for Parasitology and Tropical Medicine 2018-12 2018-12-31 /pmc/articles/PMC6327197/ /pubmed/30630279 http://dx.doi.org/10.3347/kjp.2018.56.6.583 Text en Copyright © 2018 by The Korean Society for Parasitology and Tropical Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Gou, Huitian
Xue, Huiwen
Yin, Hong
Luo, Jianxun
Sun, Xiaolin
Molecular Characterization of Hard Ticks by Cytochrome c Oxidase Subunit 1 Sequences
title Molecular Characterization of Hard Ticks by Cytochrome c Oxidase Subunit 1 Sequences
title_full Molecular Characterization of Hard Ticks by Cytochrome c Oxidase Subunit 1 Sequences
title_fullStr Molecular Characterization of Hard Ticks by Cytochrome c Oxidase Subunit 1 Sequences
title_full_unstemmed Molecular Characterization of Hard Ticks by Cytochrome c Oxidase Subunit 1 Sequences
title_short Molecular Characterization of Hard Ticks by Cytochrome c Oxidase Subunit 1 Sequences
title_sort molecular characterization of hard ticks by cytochrome c oxidase subunit 1 sequences
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327197/
https://www.ncbi.nlm.nih.gov/pubmed/30630279
http://dx.doi.org/10.3347/kjp.2018.56.6.583
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