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Characterization of CD4(+) T Cell Subsets in Patients with Abdominal Aortic Aneurysms
BACKGROUND: The mediators produced by CD4(+) T lymphocytes are involved in the pathogenesis of aneurysmal lesions in abdominal aortic aneurysm (AAA) patients. The aim of this study was to identify and characterize the CD4(+) T cell subsets involved in human AAA. METHODS: The CD4(+) T cell subsets in...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327259/ https://www.ncbi.nlm.nih.gov/pubmed/30686933 http://dx.doi.org/10.1155/2018/6967310 |
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author | Téo, Fábio Haach de Oliveira, Rômulo Tadeu Dias Villarejos, Liana Mamoni, Ronei Luciano Altemani, Albina Menezes, Fabio Husemann Blotta, Maria Heloisa Souza Lima |
author_facet | Téo, Fábio Haach de Oliveira, Rômulo Tadeu Dias Villarejos, Liana Mamoni, Ronei Luciano Altemani, Albina Menezes, Fabio Husemann Blotta, Maria Heloisa Souza Lima |
author_sort | Téo, Fábio Haach |
collection | PubMed |
description | BACKGROUND: The mediators produced by CD4(+) T lymphocytes are involved in the pathogenesis of aneurysmal lesions in abdominal aortic aneurysm (AAA) patients. The aim of this study was to identify and characterize the CD4(+) T cell subsets involved in human AAA. METHODS: The CD4(+) T cell subsets in 30 human aneurysmal lesions were determined using flow cytometry (FC) and immunohistochemistry (IHC). The peripheral blood mononuclear cells (PBMCs) from patients with AAA were also analyzed by FC and compared with control subjects. RESULTS: Human aneurysmal lesions contained IFN-γ, IL-12p35, IL-4, IL-23p19, IL-17R, and IL-22 positive cells. PBMCs from AAA patients had higher expression levels of IFN-γ, TNF-α, IL-4, and IL-22 when compared to controls. CONCLUSIONS: Our results show the presence of T(H)1, T(H)2, T(H)17, and T(H)22 subsets in aneurysmal lesions of AAA patients and suggest that these cells may be mainly activated in situ, where they can induce tissue degradation and contribute to the pathogenesis of AAA. |
format | Online Article Text |
id | pubmed-6327259 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-63272592019-01-27 Characterization of CD4(+) T Cell Subsets in Patients with Abdominal Aortic Aneurysms Téo, Fábio Haach de Oliveira, Rômulo Tadeu Dias Villarejos, Liana Mamoni, Ronei Luciano Altemani, Albina Menezes, Fabio Husemann Blotta, Maria Heloisa Souza Lima Mediators Inflamm Research Article BACKGROUND: The mediators produced by CD4(+) T lymphocytes are involved in the pathogenesis of aneurysmal lesions in abdominal aortic aneurysm (AAA) patients. The aim of this study was to identify and characterize the CD4(+) T cell subsets involved in human AAA. METHODS: The CD4(+) T cell subsets in 30 human aneurysmal lesions were determined using flow cytometry (FC) and immunohistochemistry (IHC). The peripheral blood mononuclear cells (PBMCs) from patients with AAA were also analyzed by FC and compared with control subjects. RESULTS: Human aneurysmal lesions contained IFN-γ, IL-12p35, IL-4, IL-23p19, IL-17R, and IL-22 positive cells. PBMCs from AAA patients had higher expression levels of IFN-γ, TNF-α, IL-4, and IL-22 when compared to controls. CONCLUSIONS: Our results show the presence of T(H)1, T(H)2, T(H)17, and T(H)22 subsets in aneurysmal lesions of AAA patients and suggest that these cells may be mainly activated in situ, where they can induce tissue degradation and contribute to the pathogenesis of AAA. Hindawi 2018-12-27 /pmc/articles/PMC6327259/ /pubmed/30686933 http://dx.doi.org/10.1155/2018/6967310 Text en Copyright © 2018 Fábio Haach Téo et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Téo, Fábio Haach de Oliveira, Rômulo Tadeu Dias Villarejos, Liana Mamoni, Ronei Luciano Altemani, Albina Menezes, Fabio Husemann Blotta, Maria Heloisa Souza Lima Characterization of CD4(+) T Cell Subsets in Patients with Abdominal Aortic Aneurysms |
title | Characterization of CD4(+) T Cell Subsets in Patients with Abdominal Aortic Aneurysms |
title_full | Characterization of CD4(+) T Cell Subsets in Patients with Abdominal Aortic Aneurysms |
title_fullStr | Characterization of CD4(+) T Cell Subsets in Patients with Abdominal Aortic Aneurysms |
title_full_unstemmed | Characterization of CD4(+) T Cell Subsets in Patients with Abdominal Aortic Aneurysms |
title_short | Characterization of CD4(+) T Cell Subsets in Patients with Abdominal Aortic Aneurysms |
title_sort | characterization of cd4(+) t cell subsets in patients with abdominal aortic aneurysms |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327259/ https://www.ncbi.nlm.nih.gov/pubmed/30686933 http://dx.doi.org/10.1155/2018/6967310 |
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