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Phenotypic changes of lymphocyte populations in psoriasiform dermatitis animal model

Psoriasis is a T cell mediated, chronic inflammatory autoimmune skin disease that affects up to 2–3% of the global population and leads to a decrease in quality of life. Experimental data accumulated in recent years highlighted the important role played by the immune system in the pathogenesis of th...

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Autores principales: Surcel, Mihaela, Huică, Radu-Ionuț, Munteanu, Adriana Narcisa, Isvoranu, Gheorghița, Pîrvu, Ioana Ruxandra, Ciotaru, Dan, Constantin, Carolina, Bratu, Ovidiu, Căruntu, Constantin, Neagu, Monica, Ursaciuc, Cornel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327675/
https://www.ncbi.nlm.nih.gov/pubmed/30679970
http://dx.doi.org/10.3892/etm.2018.6978
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author Surcel, Mihaela
Huică, Radu-Ionuț
Munteanu, Adriana Narcisa
Isvoranu, Gheorghița
Pîrvu, Ioana Ruxandra
Ciotaru, Dan
Constantin, Carolina
Bratu, Ovidiu
Căruntu, Constantin
Neagu, Monica
Ursaciuc, Cornel
author_facet Surcel, Mihaela
Huică, Radu-Ionuț
Munteanu, Adriana Narcisa
Isvoranu, Gheorghița
Pîrvu, Ioana Ruxandra
Ciotaru, Dan
Constantin, Carolina
Bratu, Ovidiu
Căruntu, Constantin
Neagu, Monica
Ursaciuc, Cornel
author_sort Surcel, Mihaela
collection PubMed
description Psoriasis is a T cell mediated, chronic inflammatory autoimmune skin disease that affects up to 2–3% of the global population and leads to a decrease in quality of life. Experimental data accumulated in recent years highlighted the important role played by the immune system in the pathogenesis of this disease. Non-human psoriasis models are an important research tool that attempts to reproduce the clinical features of the disease in order to explain the pathogenesis of psoriasis and to identify possible therapeutic targets. Imiquimod-based murine model of psoriatic dermatitis is an alternative to traditional models of experimental psoriasis in mice and the induced dermatitis closely mimics the pathologic changes in human psoriasis. In order to emphasize changes in immune cell populations involved in lesion pathogenesis, we performed a murine model of psoriasiform dermatitis model by topical IMQ application. The progress and the severity of IMQ-induced skin inflammation were clinically (PASI score) and histopathologically evaluated. The immunological changes induced by IMQ treatment in lymphocyte populations from peripheral blood and spleen were evaluated by flow cytometry. The main changes observed in peripheral blood were the significantly increased T-CD8a(+) lymphocyte and NK1.1(+) cell percentages and the decreased T-CD4(+) and B lymphocyte percentages in IMQ-treated mice. In spleen samples, lymphocytes showed the same tendency of variation as in peripheral blood, but without statistical significance. A significant decrease of B cells percentages was observed in spleen suspensions. Data obtained in skin samples may suggest the involvement of CD3ε(+), CD4(+) and CD8a(+) cells in the lesional process. This murine model was analyzed by performing a basic cellular profile at three levels: peripheral blood, spleen and skin. The evaluation aimed to establish the immune framework of this experimental model that could further be used for etipathogenic mechanism identification and/or for studies regarding targeted therapies.
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spelling pubmed-63276752019-01-24 Phenotypic changes of lymphocyte populations in psoriasiform dermatitis animal model Surcel, Mihaela Huică, Radu-Ionuț Munteanu, Adriana Narcisa Isvoranu, Gheorghița Pîrvu, Ioana Ruxandra Ciotaru, Dan Constantin, Carolina Bratu, Ovidiu Căruntu, Constantin Neagu, Monica Ursaciuc, Cornel Exp Ther Med Articles Psoriasis is a T cell mediated, chronic inflammatory autoimmune skin disease that affects up to 2–3% of the global population and leads to a decrease in quality of life. Experimental data accumulated in recent years highlighted the important role played by the immune system in the pathogenesis of this disease. Non-human psoriasis models are an important research tool that attempts to reproduce the clinical features of the disease in order to explain the pathogenesis of psoriasis and to identify possible therapeutic targets. Imiquimod-based murine model of psoriatic dermatitis is an alternative to traditional models of experimental psoriasis in mice and the induced dermatitis closely mimics the pathologic changes in human psoriasis. In order to emphasize changes in immune cell populations involved in lesion pathogenesis, we performed a murine model of psoriasiform dermatitis model by topical IMQ application. The progress and the severity of IMQ-induced skin inflammation were clinically (PASI score) and histopathologically evaluated. The immunological changes induced by IMQ treatment in lymphocyte populations from peripheral blood and spleen were evaluated by flow cytometry. The main changes observed in peripheral blood were the significantly increased T-CD8a(+) lymphocyte and NK1.1(+) cell percentages and the decreased T-CD4(+) and B lymphocyte percentages in IMQ-treated mice. In spleen samples, lymphocytes showed the same tendency of variation as in peripheral blood, but without statistical significance. A significant decrease of B cells percentages was observed in spleen suspensions. Data obtained in skin samples may suggest the involvement of CD3ε(+), CD4(+) and CD8a(+) cells in the lesional process. This murine model was analyzed by performing a basic cellular profile at three levels: peripheral blood, spleen and skin. The evaluation aimed to establish the immune framework of this experimental model that could further be used for etipathogenic mechanism identification and/or for studies regarding targeted therapies. D.A. Spandidos 2019-02 2018-11-16 /pmc/articles/PMC6327675/ /pubmed/30679970 http://dx.doi.org/10.3892/etm.2018.6978 Text en Copyright: © Surcel et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Surcel, Mihaela
Huică, Radu-Ionuț
Munteanu, Adriana Narcisa
Isvoranu, Gheorghița
Pîrvu, Ioana Ruxandra
Ciotaru, Dan
Constantin, Carolina
Bratu, Ovidiu
Căruntu, Constantin
Neagu, Monica
Ursaciuc, Cornel
Phenotypic changes of lymphocyte populations in psoriasiform dermatitis animal model
title Phenotypic changes of lymphocyte populations in psoriasiform dermatitis animal model
title_full Phenotypic changes of lymphocyte populations in psoriasiform dermatitis animal model
title_fullStr Phenotypic changes of lymphocyte populations in psoriasiform dermatitis animal model
title_full_unstemmed Phenotypic changes of lymphocyte populations in psoriasiform dermatitis animal model
title_short Phenotypic changes of lymphocyte populations in psoriasiform dermatitis animal model
title_sort phenotypic changes of lymphocyte populations in psoriasiform dermatitis animal model
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327675/
https://www.ncbi.nlm.nih.gov/pubmed/30679970
http://dx.doi.org/10.3892/etm.2018.6978
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