A feature-based analysis identifies COL1A2 as a regulator in pancreatic cancer

This study aimed to identify genetic biomarkers in pancreatic cancer (PC) and explore its function in PC via a feature-base analysis of bioinformatics. OMIM and DisGeNET databases discovered 209 PC connected genes and then 516 connected genes were identified. We selected 29 genes according to optima...

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Autores principales: Wu, Jie, Liu, Jing, Wei, XiaoQing, Yu, Qi, Niu, XiangHuan, Tang, ShuHong, Song, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327995/
https://www.ncbi.nlm.nih.gov/pubmed/30734598
http://dx.doi.org/10.1080/14756366.2018.1484734
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author Wu, Jie
Liu, Jing
Wei, XiaoQing
Yu, Qi
Niu, XiangHuan
Tang, ShuHong
Song, Lei
author_facet Wu, Jie
Liu, Jing
Wei, XiaoQing
Yu, Qi
Niu, XiangHuan
Tang, ShuHong
Song, Lei
author_sort Wu, Jie
collection PubMed
description This study aimed to identify genetic biomarkers in pancreatic cancer (PC) and explore its function in PC via a feature-base analysis of bioinformatics. OMIM and DisGeNET databases discovered 209 PC connected genes and then 516 connected genes were identified. We selected 29 genes according to optimal features and chose COL1A2, which had the highest expression, for the following experiment. The expression of COL1A2 was determined by qRT-PCR; cell proliferation was determined by MTT assay; migration and invasion after COL1A2 and miR-25-3p transfection was evaluated by Transwell assay. COL1A2 presented the highest expression in PC tissues, which was validated in functional experiments. MiR-25-3p suppressed the expression of COL1A2 in cell lines and inhibited migration, invasion and proliferation of PC cells. MiR-25-3p could suppress the expression of COL1A2 and inhibit the proliferation, migration and invasion of PC cells which provided a new idea for the detection and treatment of PC.
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spelling pubmed-63279952019-01-16 A feature-based analysis identifies COL1A2 as a regulator in pancreatic cancer Wu, Jie Liu, Jing Wei, XiaoQing Yu, Qi Niu, XiangHuan Tang, ShuHong Song, Lei J Enzyme Inhib Med Chem Research Paper This study aimed to identify genetic biomarkers in pancreatic cancer (PC) and explore its function in PC via a feature-base analysis of bioinformatics. OMIM and DisGeNET databases discovered 209 PC connected genes and then 516 connected genes were identified. We selected 29 genes according to optimal features and chose COL1A2, which had the highest expression, for the following experiment. The expression of COL1A2 was determined by qRT-PCR; cell proliferation was determined by MTT assay; migration and invasion after COL1A2 and miR-25-3p transfection was evaluated by Transwell assay. COL1A2 presented the highest expression in PC tissues, which was validated in functional experiments. MiR-25-3p suppressed the expression of COL1A2 in cell lines and inhibited migration, invasion and proliferation of PC cells. MiR-25-3p could suppress the expression of COL1A2 and inhibit the proliferation, migration and invasion of PC cells which provided a new idea for the detection and treatment of PC. Taylor & Francis 2019-01-07 /pmc/articles/PMC6327995/ /pubmed/30734598 http://dx.doi.org/10.1080/14756366.2018.1484734 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Wu, Jie
Liu, Jing
Wei, XiaoQing
Yu, Qi
Niu, XiangHuan
Tang, ShuHong
Song, Lei
A feature-based analysis identifies COL1A2 as a regulator in pancreatic cancer
title A feature-based analysis identifies COL1A2 as a regulator in pancreatic cancer
title_full A feature-based analysis identifies COL1A2 as a regulator in pancreatic cancer
title_fullStr A feature-based analysis identifies COL1A2 as a regulator in pancreatic cancer
title_full_unstemmed A feature-based analysis identifies COL1A2 as a regulator in pancreatic cancer
title_short A feature-based analysis identifies COL1A2 as a regulator in pancreatic cancer
title_sort feature-based analysis identifies col1a2 as a regulator in pancreatic cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327995/
https://www.ncbi.nlm.nih.gov/pubmed/30734598
http://dx.doi.org/10.1080/14756366.2018.1484734
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