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Delayed differentiation of vaginal and uterine microbiomes in dairy cows developing postpartum endometritis
Bacterial overgrowth in the uterus is a normal event after parturition. In contrast to the healthy cow, animals unable to control the infection within 21 days after calving develop postpartum endometritis. Studies on the Microbial Ecology of the bovine reproductive tract have focused on either vagin...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328119/ https://www.ncbi.nlm.nih.gov/pubmed/30629579 http://dx.doi.org/10.1371/journal.pone.0200974 |
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author | Miranda-CasoLuengo, Raúl Lu, Junnan Williams, Erin J. Miranda-CasoLuengo, Aleksandra A. Carrington, Stephen D. Evans, Alexander C. O. Meijer, Wim G. |
author_facet | Miranda-CasoLuengo, Raúl Lu, Junnan Williams, Erin J. Miranda-CasoLuengo, Aleksandra A. Carrington, Stephen D. Evans, Alexander C. O. Meijer, Wim G. |
author_sort | Miranda-CasoLuengo, Raúl |
collection | PubMed |
description | Bacterial overgrowth in the uterus is a normal event after parturition. In contrast to the healthy cow, animals unable to control the infection within 21 days after calving develop postpartum endometritis. Studies on the Microbial Ecology of the bovine reproductive tract have focused on either vaginal or uterine microbiomes. This is the first study that compares both microbiomes in the same animals. Terminal Restriction Fragment Length Polymorphism of the 16S rRNA gene showed that despite large differences associated to individuals, a shared community exist in vagina and uterus during the postpartum period. The largest changes associated with development of endometritis were observed at 7 days postpartum, a time when vaginal and uterine microbiomes were most similar. 16S rRNA pyrosequencing of the vaginal microbiome at 7 days postpartum showed at least three different microbiome types that were associated with later development of postpartum endometritis. All three microbiome types featured reduced bacterial diversity. Taken together, the above findings support a scenario where disruption of the compartmentalization of the reproductive tract during parturition results in the dispersal and mixing of the vaginal and uterine microbiomes, which subsequently are subject to differentiation. This differentiation was observed early postpartum in the healthy cow. In contrast, loss of bacterial diversity and dominance of the microbiome by few bacterial taxa were related to a delayed succession at 7DPP in cows that at 21 DPP or later were diagnosed with endometritis. |
format | Online Article Text |
id | pubmed-6328119 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-63281192019-02-01 Delayed differentiation of vaginal and uterine microbiomes in dairy cows developing postpartum endometritis Miranda-CasoLuengo, Raúl Lu, Junnan Williams, Erin J. Miranda-CasoLuengo, Aleksandra A. Carrington, Stephen D. Evans, Alexander C. O. Meijer, Wim G. PLoS One Research Article Bacterial overgrowth in the uterus is a normal event after parturition. In contrast to the healthy cow, animals unable to control the infection within 21 days after calving develop postpartum endometritis. Studies on the Microbial Ecology of the bovine reproductive tract have focused on either vaginal or uterine microbiomes. This is the first study that compares both microbiomes in the same animals. Terminal Restriction Fragment Length Polymorphism of the 16S rRNA gene showed that despite large differences associated to individuals, a shared community exist in vagina and uterus during the postpartum period. The largest changes associated with development of endometritis were observed at 7 days postpartum, a time when vaginal and uterine microbiomes were most similar. 16S rRNA pyrosequencing of the vaginal microbiome at 7 days postpartum showed at least three different microbiome types that were associated with later development of postpartum endometritis. All three microbiome types featured reduced bacterial diversity. Taken together, the above findings support a scenario where disruption of the compartmentalization of the reproductive tract during parturition results in the dispersal and mixing of the vaginal and uterine microbiomes, which subsequently are subject to differentiation. This differentiation was observed early postpartum in the healthy cow. In contrast, loss of bacterial diversity and dominance of the microbiome by few bacterial taxa were related to a delayed succession at 7DPP in cows that at 21 DPP or later were diagnosed with endometritis. Public Library of Science 2019-01-10 /pmc/articles/PMC6328119/ /pubmed/30629579 http://dx.doi.org/10.1371/journal.pone.0200974 Text en © 2019 Miranda-CasoLuengo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Miranda-CasoLuengo, Raúl Lu, Junnan Williams, Erin J. Miranda-CasoLuengo, Aleksandra A. Carrington, Stephen D. Evans, Alexander C. O. Meijer, Wim G. Delayed differentiation of vaginal and uterine microbiomes in dairy cows developing postpartum endometritis |
title | Delayed differentiation of vaginal and uterine microbiomes in dairy cows developing postpartum endometritis |
title_full | Delayed differentiation of vaginal and uterine microbiomes in dairy cows developing postpartum endometritis |
title_fullStr | Delayed differentiation of vaginal and uterine microbiomes in dairy cows developing postpartum endometritis |
title_full_unstemmed | Delayed differentiation of vaginal and uterine microbiomes in dairy cows developing postpartum endometritis |
title_short | Delayed differentiation of vaginal and uterine microbiomes in dairy cows developing postpartum endometritis |
title_sort | delayed differentiation of vaginal and uterine microbiomes in dairy cows developing postpartum endometritis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328119/ https://www.ncbi.nlm.nih.gov/pubmed/30629579 http://dx.doi.org/10.1371/journal.pone.0200974 |
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