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LC–ESI–IT-MS/MS and MALDI-TOF Approach: Identification of Natural Polymers from Rhizophora mangle Barks and Determination of Their Analgesic and Anti-inflammatory Properties
ABSTRACT: We recognize the chemical composition of the acetonic extract of Rhizophora mangle barks (AERM) using mass spectrometry analysis [liquid chromatography (LC)–ESI–IT-MS/MS and matrix-assisted laser desorption/ionization-time of flight-MS (MALDI-TOF)]. Analgesic activity was evaluated by form...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Singapore
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328426/ https://www.ncbi.nlm.nih.gov/pubmed/30430388 http://dx.doi.org/10.1007/s13659-018-0192-8 |
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author | de Souza Mesquita, Leonardo Mendes Rodrigues, Caroline Fabri Bittencourt da Rocha, Cláudia Quintino Bianchim, Mayara Silveira Rodrigues, Clenilson Martins de Oliveira, Vanda Maria Gaeta, Henrique Hessel Belchor, Mariana Novo Toyama, Marcos Hikari Vilegas, Wagner |
author_facet | de Souza Mesquita, Leonardo Mendes Rodrigues, Caroline Fabri Bittencourt da Rocha, Cláudia Quintino Bianchim, Mayara Silveira Rodrigues, Clenilson Martins de Oliveira, Vanda Maria Gaeta, Henrique Hessel Belchor, Mariana Novo Toyama, Marcos Hikari Vilegas, Wagner |
author_sort | de Souza Mesquita, Leonardo Mendes |
collection | PubMed |
description | ABSTRACT: We recognize the chemical composition of the acetonic extract of Rhizophora mangle barks (AERM) using mass spectrometry analysis [liquid chromatography (LC)–ESI–IT-MS/MS and matrix-assisted laser desorption/ionization-time of flight-MS (MALDI-TOF)]. Analgesic activity was evaluated by formalin and tail-flick experimental assays. Anti-inflammatory activity was performed by paw edema test induced by carrageenan and 48/80 compounds. The first series of experiments involved [LC]–FIA–IT-MS/MS with 11 separated catechins derivatives until degree of polymerization 3 (DP3). The spectra obtained by MALDI-TOF analysis of the AERM presented two homologous series: one based on polymers of m/z 288 Da increments (up to DP12) and another series based on polymers of m/z [288 + 162] Da increments (up to DP11). In addition to these series of flavan-3-ol, each DP had a subset of masses with a variation of − 16 Da (homologous series of afzelechins—m/z 873–3465 Da) and + 16 Da (homologous series of gallocatechins—m/z 905–3497 Da). A similar pattern with homologous series of gallocatechins and afzelechins could also be observed for a fifth and a sixth monohexoside series: glucogallocatechins (m/z 779–3371) and glucoafzelechins (m/z 747–3339). The intraperitoneal administration of different doses of AERM (50, 150 and 300 µg mL(−1)) have a morphine-like effect and intense anti-inflammatory activity. GRAPHICAL ABSTRACT: [Image: see text] |
format | Online Article Text |
id | pubmed-6328426 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-63284262019-01-25 LC–ESI–IT-MS/MS and MALDI-TOF Approach: Identification of Natural Polymers from Rhizophora mangle Barks and Determination of Their Analgesic and Anti-inflammatory Properties de Souza Mesquita, Leonardo Mendes Rodrigues, Caroline Fabri Bittencourt da Rocha, Cláudia Quintino Bianchim, Mayara Silveira Rodrigues, Clenilson Martins de Oliveira, Vanda Maria Gaeta, Henrique Hessel Belchor, Mariana Novo Toyama, Marcos Hikari Vilegas, Wagner Nat Prod Bioprospect Original Article ABSTRACT: We recognize the chemical composition of the acetonic extract of Rhizophora mangle barks (AERM) using mass spectrometry analysis [liquid chromatography (LC)–ESI–IT-MS/MS and matrix-assisted laser desorption/ionization-time of flight-MS (MALDI-TOF)]. Analgesic activity was evaluated by formalin and tail-flick experimental assays. Anti-inflammatory activity was performed by paw edema test induced by carrageenan and 48/80 compounds. The first series of experiments involved [LC]–FIA–IT-MS/MS with 11 separated catechins derivatives until degree of polymerization 3 (DP3). The spectra obtained by MALDI-TOF analysis of the AERM presented two homologous series: one based on polymers of m/z 288 Da increments (up to DP12) and another series based on polymers of m/z [288 + 162] Da increments (up to DP11). In addition to these series of flavan-3-ol, each DP had a subset of masses with a variation of − 16 Da (homologous series of afzelechins—m/z 873–3465 Da) and + 16 Da (homologous series of gallocatechins—m/z 905–3497 Da). A similar pattern with homologous series of gallocatechins and afzelechins could also be observed for a fifth and a sixth monohexoside series: glucogallocatechins (m/z 779–3371) and glucoafzelechins (m/z 747–3339). The intraperitoneal administration of different doses of AERM (50, 150 and 300 µg mL(−1)) have a morphine-like effect and intense anti-inflammatory activity. GRAPHICAL ABSTRACT: [Image: see text] Springer Singapore 2018-11-14 /pmc/articles/PMC6328426/ /pubmed/30430388 http://dx.doi.org/10.1007/s13659-018-0192-8 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article de Souza Mesquita, Leonardo Mendes Rodrigues, Caroline Fabri Bittencourt da Rocha, Cláudia Quintino Bianchim, Mayara Silveira Rodrigues, Clenilson Martins de Oliveira, Vanda Maria Gaeta, Henrique Hessel Belchor, Mariana Novo Toyama, Marcos Hikari Vilegas, Wagner LC–ESI–IT-MS/MS and MALDI-TOF Approach: Identification of Natural Polymers from Rhizophora mangle Barks and Determination of Their Analgesic and Anti-inflammatory Properties |
title | LC–ESI–IT-MS/MS and MALDI-TOF Approach: Identification of Natural Polymers from Rhizophora mangle Barks and Determination of Their Analgesic and Anti-inflammatory Properties |
title_full | LC–ESI–IT-MS/MS and MALDI-TOF Approach: Identification of Natural Polymers from Rhizophora mangle Barks and Determination of Their Analgesic and Anti-inflammatory Properties |
title_fullStr | LC–ESI–IT-MS/MS and MALDI-TOF Approach: Identification of Natural Polymers from Rhizophora mangle Barks and Determination of Their Analgesic and Anti-inflammatory Properties |
title_full_unstemmed | LC–ESI–IT-MS/MS and MALDI-TOF Approach: Identification of Natural Polymers from Rhizophora mangle Barks and Determination of Their Analgesic and Anti-inflammatory Properties |
title_short | LC–ESI–IT-MS/MS and MALDI-TOF Approach: Identification of Natural Polymers from Rhizophora mangle Barks and Determination of Their Analgesic and Anti-inflammatory Properties |
title_sort | lc–esi–it-ms/ms and maldi-tof approach: identification of natural polymers from rhizophora mangle barks and determination of their analgesic and anti-inflammatory properties |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328426/ https://www.ncbi.nlm.nih.gov/pubmed/30430388 http://dx.doi.org/10.1007/s13659-018-0192-8 |
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