Crystal Structure of Photosystem I Monomer From Synechocystis PCC 6803

A single histidine addition to the C-terminus of PsaL of Synechocystis sp. PCC 6803 was previously reported by our lab to shift the trimer-to-monomer ratio of PSI in favor of the monomeric form. P700 re-reduction and NADP(+) photo-reduction measurements of the PsaL(HIS) strain show no effect on PSI...

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Detalles Bibliográficos
Autores principales: Netzer-El, Sigal Yoli, Caspy, Ido, Nelson, Nathan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328476/
https://www.ncbi.nlm.nih.gov/pubmed/30662446
http://dx.doi.org/10.3389/fpls.2018.01865
Descripción
Sumario:A single histidine addition to the C-terminus of PsaL of Synechocystis sp. PCC 6803 was previously reported by our lab to shift the trimer-to-monomer ratio of PSI in favor of the monomeric form. P700 re-reduction and NADP(+) photo-reduction measurements of the PsaL(HIS) strain show no effect on PSI activity in comparison to the WT trimeric PSI. Crystal structure of the PsaL(HIS) monomeric PSI reveals several alterations that occurred in the trimerisation site of PSI, primarily a deformation of the C-terminus of PsaL and loss of chlorophyll a and β-carotene molecules.

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