Crystal Structure of Photosystem I Monomer From Synechocystis PCC 6803

A single histidine addition to the C-terminus of PsaL of Synechocystis sp. PCC 6803 was previously reported by our lab to shift the trimer-to-monomer ratio of PSI in favor of the monomeric form. P700 re-reduction and NADP(+) photo-reduction measurements of the PsaL(HIS) strain show no effect on PSI...

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Autores principales: Netzer-El, Sigal Yoli, Caspy, Ido, Nelson, Nathan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328476/
https://www.ncbi.nlm.nih.gov/pubmed/30662446
http://dx.doi.org/10.3389/fpls.2018.01865
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author Netzer-El, Sigal Yoli
Caspy, Ido
Nelson, Nathan
author_facet Netzer-El, Sigal Yoli
Caspy, Ido
Nelson, Nathan
author_sort Netzer-El, Sigal Yoli
collection PubMed
description A single histidine addition to the C-terminus of PsaL of Synechocystis sp. PCC 6803 was previously reported by our lab to shift the trimer-to-monomer ratio of PSI in favor of the monomeric form. P700 re-reduction and NADP(+) photo-reduction measurements of the PsaL(HIS) strain show no effect on PSI activity in comparison to the WT trimeric PSI. Crystal structure of the PsaL(HIS) monomeric PSI reveals several alterations that occurred in the trimerisation site of PSI, primarily a deformation of the C-terminus of PsaL and loss of chlorophyll a and β-carotene molecules.
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spelling pubmed-63284762019-01-18 Crystal Structure of Photosystem I Monomer From Synechocystis PCC 6803 Netzer-El, Sigal Yoli Caspy, Ido Nelson, Nathan Front Plant Sci Plant Science A single histidine addition to the C-terminus of PsaL of Synechocystis sp. PCC 6803 was previously reported by our lab to shift the trimer-to-monomer ratio of PSI in favor of the monomeric form. P700 re-reduction and NADP(+) photo-reduction measurements of the PsaL(HIS) strain show no effect on PSI activity in comparison to the WT trimeric PSI. Crystal structure of the PsaL(HIS) monomeric PSI reveals several alterations that occurred in the trimerisation site of PSI, primarily a deformation of the C-terminus of PsaL and loss of chlorophyll a and β-carotene molecules. Frontiers Media S.A. 2019-01-04 /pmc/articles/PMC6328476/ /pubmed/30662446 http://dx.doi.org/10.3389/fpls.2018.01865 Text en Copyright © 2019 Netzer-El, Caspy and Nelson. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Netzer-El, Sigal Yoli
Caspy, Ido
Nelson, Nathan
Crystal Structure of Photosystem I Monomer From Synechocystis PCC 6803
title Crystal Structure of Photosystem I Monomer From Synechocystis PCC 6803
title_full Crystal Structure of Photosystem I Monomer From Synechocystis PCC 6803
title_fullStr Crystal Structure of Photosystem I Monomer From Synechocystis PCC 6803
title_full_unstemmed Crystal Structure of Photosystem I Monomer From Synechocystis PCC 6803
title_short Crystal Structure of Photosystem I Monomer From Synechocystis PCC 6803
title_sort crystal structure of photosystem i monomer from synechocystis pcc 6803
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328476/
https://www.ncbi.nlm.nih.gov/pubmed/30662446
http://dx.doi.org/10.3389/fpls.2018.01865
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