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Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco

Water deficit is a key limiting factor that affects the growth, development and productivity of crops. It is vital to understand the mechanisms by which plants respond to drought stress. Here an N-acetylglutamate kinase gene, ZmNAGK, was cloned from maize (Zea mays). ZmNAGK was expressed at high lev...

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Autores principales: Liu, Weijuan, Xiang, Yang, Zhang, Xiaoyun, Han, Gaoqiang, Sun, Xiujuan, Sheng, Yu, Yan, Jingwei, Scheller, Henrik Vibe, Zhang, Aying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328498/
https://www.ncbi.nlm.nih.gov/pubmed/30662448
http://dx.doi.org/10.3389/fpls.2018.01902
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author Liu, Weijuan
Xiang, Yang
Zhang, Xiaoyun
Han, Gaoqiang
Sun, Xiujuan
Sheng, Yu
Yan, Jingwei
Scheller, Henrik Vibe
Zhang, Aying
author_facet Liu, Weijuan
Xiang, Yang
Zhang, Xiaoyun
Han, Gaoqiang
Sun, Xiujuan
Sheng, Yu
Yan, Jingwei
Scheller, Henrik Vibe
Zhang, Aying
author_sort Liu, Weijuan
collection PubMed
description Water deficit is a key limiting factor that affects the growth, development and productivity of crops. It is vital to understand the mechanisms by which plants respond to drought stress. Here an N-acetylglutamate kinase gene, ZmNAGK, was cloned from maize (Zea mays). ZmNAGK was expressed at high levels in maize leaves and at lower levels in root, stem, female flower and male flower. The expression of ZmNAGK was significantly induced by PEG, NaCl, ABA, brassinosteroid and H(2)O(2). The ectopic expression of ZmNAGK in tobacco resulted in higher tolerance to drought compared to plants transformed with empty vector. Further physiological analysis revealed that overexpression of ZmNAGK could enhance the activities of antioxidant defense enzymes, and decrease malondialdehyde content and leakage of electrolyte in tobacco under drought stress. Moreover, the ZmNAGK transgenic tobacco accumulated more arginine and nitric oxide (NO) than control plants under drought stress. In addition, the ZmNAGK transgenic tobaccos activated drought responses faster than vector-transformed plants. These results indicate that ZmNAGK can play a vital role in enhancing drought tolerance by likely affecting the arginine and NO accumulation, and ZmNAGK could be involved in different strategies in response to drought stress.
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spelling pubmed-63284982019-01-18 Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco Liu, Weijuan Xiang, Yang Zhang, Xiaoyun Han, Gaoqiang Sun, Xiujuan Sheng, Yu Yan, Jingwei Scheller, Henrik Vibe Zhang, Aying Front Plant Sci Plant Science Water deficit is a key limiting factor that affects the growth, development and productivity of crops. It is vital to understand the mechanisms by which plants respond to drought stress. Here an N-acetylglutamate kinase gene, ZmNAGK, was cloned from maize (Zea mays). ZmNAGK was expressed at high levels in maize leaves and at lower levels in root, stem, female flower and male flower. The expression of ZmNAGK was significantly induced by PEG, NaCl, ABA, brassinosteroid and H(2)O(2). The ectopic expression of ZmNAGK in tobacco resulted in higher tolerance to drought compared to plants transformed with empty vector. Further physiological analysis revealed that overexpression of ZmNAGK could enhance the activities of antioxidant defense enzymes, and decrease malondialdehyde content and leakage of electrolyte in tobacco under drought stress. Moreover, the ZmNAGK transgenic tobacco accumulated more arginine and nitric oxide (NO) than control plants under drought stress. In addition, the ZmNAGK transgenic tobaccos activated drought responses faster than vector-transformed plants. These results indicate that ZmNAGK can play a vital role in enhancing drought tolerance by likely affecting the arginine and NO accumulation, and ZmNAGK could be involved in different strategies in response to drought stress. Frontiers Media S.A. 2019-01-04 /pmc/articles/PMC6328498/ /pubmed/30662448 http://dx.doi.org/10.3389/fpls.2018.01902 Text en Copyright © 2019 Liu, Xiang, Zhang, Han, Sun, Sheng, Yan, Scheller and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Liu, Weijuan
Xiang, Yang
Zhang, Xiaoyun
Han, Gaoqiang
Sun, Xiujuan
Sheng, Yu
Yan, Jingwei
Scheller, Henrik Vibe
Zhang, Aying
Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco
title Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco
title_full Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco
title_fullStr Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco
title_full_unstemmed Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco
title_short Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco
title_sort over-expression of a maize n-acetylglutamate kinase gene (zmnagk) improves drought tolerance in tobacco
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328498/
https://www.ncbi.nlm.nih.gov/pubmed/30662448
http://dx.doi.org/10.3389/fpls.2018.01902
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