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Regulation of Myosin-5b by Rab11a and the Rab11 family interacting protein 2

Mammalian myosin-5b (Myo5b) plays a critical role in the recycling of endosomes to the plasma membrane via the interactions with Rab11a and the Rab11 family interacting protein 2 (FIP2). However, it remains unclear on how Rab11a and FIP2 are coordinated in tethering Myo5b with the vesicles and activ...

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Autores principales: Ji, Huan-Hong, Yao, Lin-Lin, Liu, Chang, Li, Xiang-dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328864/
https://www.ncbi.nlm.nih.gov/pubmed/30545898
http://dx.doi.org/10.1042/BSR20181252
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author Ji, Huan-Hong
Yao, Lin-Lin
Liu, Chang
Li, Xiang-dong
author_facet Ji, Huan-Hong
Yao, Lin-Lin
Liu, Chang
Li, Xiang-dong
author_sort Ji, Huan-Hong
collection PubMed
description Mammalian myosin-5b (Myo5b) plays a critical role in the recycling of endosomes to the plasma membrane via the interactions with Rab11a and the Rab11 family interacting protein 2 (FIP2). However, it remains unclear on how Rab11a and FIP2 are coordinated in tethering Myo5b with the vesicles and activating the motor function of Myo5b. In the present study, we show that Rab11a binds to the globular tail domain (GTD) of Myo5b and this binding abolishes the head–GTD interaction of Myo5b, thus activating the motor function of Myo5b. On the other hand, FIP2 directly interacts with both Rab11a and the tail of Myo5b, and the binding of FIP2 to Myo5b does not affect Myo5b motor function. Moreover, Rab11a displays higher affinity to FIP2 than to Myo5b, suggesting that Rab11a binds preferentially to FIP2 than to Myo5b. Based on the current findings, we propose that the association of Myo5b with vesicles is mediated by FIP2, which bridges Myo5b and the membrane-bound Rab11a, whereas the motor function of Myo5b is regulated by Rab11a.
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spelling pubmed-63288642019-01-18 Regulation of Myosin-5b by Rab11a and the Rab11 family interacting protein 2 Ji, Huan-Hong Yao, Lin-Lin Liu, Chang Li, Xiang-dong Biosci Rep Research Articles Mammalian myosin-5b (Myo5b) plays a critical role in the recycling of endosomes to the plasma membrane via the interactions with Rab11a and the Rab11 family interacting protein 2 (FIP2). However, it remains unclear on how Rab11a and FIP2 are coordinated in tethering Myo5b with the vesicles and activating the motor function of Myo5b. In the present study, we show that Rab11a binds to the globular tail domain (GTD) of Myo5b and this binding abolishes the head–GTD interaction of Myo5b, thus activating the motor function of Myo5b. On the other hand, FIP2 directly interacts with both Rab11a and the tail of Myo5b, and the binding of FIP2 to Myo5b does not affect Myo5b motor function. Moreover, Rab11a displays higher affinity to FIP2 than to Myo5b, suggesting that Rab11a binds preferentially to FIP2 than to Myo5b. Based on the current findings, we propose that the association of Myo5b with vesicles is mediated by FIP2, which bridges Myo5b and the membrane-bound Rab11a, whereas the motor function of Myo5b is regulated by Rab11a. Portland Press Ltd. 2019-01-08 /pmc/articles/PMC6328864/ /pubmed/30545898 http://dx.doi.org/10.1042/BSR20181252 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Ji, Huan-Hong
Yao, Lin-Lin
Liu, Chang
Li, Xiang-dong
Regulation of Myosin-5b by Rab11a and the Rab11 family interacting protein 2
title Regulation of Myosin-5b by Rab11a and the Rab11 family interacting protein 2
title_full Regulation of Myosin-5b by Rab11a and the Rab11 family interacting protein 2
title_fullStr Regulation of Myosin-5b by Rab11a and the Rab11 family interacting protein 2
title_full_unstemmed Regulation of Myosin-5b by Rab11a and the Rab11 family interacting protein 2
title_short Regulation of Myosin-5b by Rab11a and the Rab11 family interacting protein 2
title_sort regulation of myosin-5b by rab11a and the rab11 family interacting protein 2
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328864/
https://www.ncbi.nlm.nih.gov/pubmed/30545898
http://dx.doi.org/10.1042/BSR20181252
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