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Estrogen ameliorates allergic airway inflammation by regulating activation of NLRP3 in mice
Background: Estrogen has been suggested to play a protective role against airway inflammations, such as asthma. In these processes, the inflammasome nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain containing 3 (NLRP3) partly accounts for the activation of pro-inflamma...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328879/ https://www.ncbi.nlm.nih.gov/pubmed/30373775 http://dx.doi.org/10.1042/BSR20181117 |
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author | Cheng, Cheng Wu, Huimei Wang, Muzi Wang, Lixia Zou, Hongyun Li, Shuai Liu, Rongyu |
author_facet | Cheng, Cheng Wu, Huimei Wang, Muzi Wang, Lixia Zou, Hongyun Li, Shuai Liu, Rongyu |
author_sort | Cheng, Cheng |
collection | PubMed |
description | Background: Estrogen has been suggested to play a protective role against airway inflammations, such as asthma. In these processes, the inflammasome nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain containing 3 (NLRP3) partly accounts for the activation of pro-inflammatory factors. The aim of the present study was to investigate whether NLRP3 was involved in the protective effect of estrogen against allergic airway inflammation. Methods: An ovariectomy was performed on female C57BL/6 mice; some were sham-operated (sham). We then sensitized and challenged them with ovalbumin (OVA) to establish an airway inflammation model. Meanwhile, some mice were treated with 17β-estradiol (E2) for 28 days. Results: The expression of NLRP3 inflammasome and its downstream products, caspase-1 and the pro-inflammatory cytokine interleukin (IL)-1β (IL-1β), increased concomitantly with OVA-challenged airway inflammation and decreased with the expression of estrogen receptor β (ERβ). In addition, treating ovariectomized (OVX) mice with E2 dramatically ameliorated airway inflammation via such mechanisms as leukocyte recruitment, mucus production, and secretion of pro-inflammatory cytokines other than IL-18 in bronchoalveolar lavage (BAL) fluid (BALF). Furthermore, E2 suppressed both the mRNA expression and protein expression of NLRP3, caspase-1, and IL-1β. In summary, our study showed that NLRP3 inflammasome activation and pro-inflammatory cytokine production markedly increased in OVA-induced airway inflammation, and E2 effectively abrogated such inflammation by regulating the activation of NLRP3. |
format | Online Article Text |
id | pubmed-6328879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63288792019-01-18 Estrogen ameliorates allergic airway inflammation by regulating activation of NLRP3 in mice Cheng, Cheng Wu, Huimei Wang, Muzi Wang, Lixia Zou, Hongyun Li, Shuai Liu, Rongyu Biosci Rep Research Articles Background: Estrogen has been suggested to play a protective role against airway inflammations, such as asthma. In these processes, the inflammasome nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain containing 3 (NLRP3) partly accounts for the activation of pro-inflammatory factors. The aim of the present study was to investigate whether NLRP3 was involved in the protective effect of estrogen against allergic airway inflammation. Methods: An ovariectomy was performed on female C57BL/6 mice; some were sham-operated (sham). We then sensitized and challenged them with ovalbumin (OVA) to establish an airway inflammation model. Meanwhile, some mice were treated with 17β-estradiol (E2) for 28 days. Results: The expression of NLRP3 inflammasome and its downstream products, caspase-1 and the pro-inflammatory cytokine interleukin (IL)-1β (IL-1β), increased concomitantly with OVA-challenged airway inflammation and decreased with the expression of estrogen receptor β (ERβ). In addition, treating ovariectomized (OVX) mice with E2 dramatically ameliorated airway inflammation via such mechanisms as leukocyte recruitment, mucus production, and secretion of pro-inflammatory cytokines other than IL-18 in bronchoalveolar lavage (BAL) fluid (BALF). Furthermore, E2 suppressed both the mRNA expression and protein expression of NLRP3, caspase-1, and IL-1β. In summary, our study showed that NLRP3 inflammasome activation and pro-inflammatory cytokine production markedly increased in OVA-induced airway inflammation, and E2 effectively abrogated such inflammation by regulating the activation of NLRP3. Portland Press Ltd. 2019-01-08 /pmc/articles/PMC6328879/ /pubmed/30373775 http://dx.doi.org/10.1042/BSR20181117 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Cheng, Cheng Wu, Huimei Wang, Muzi Wang, Lixia Zou, Hongyun Li, Shuai Liu, Rongyu Estrogen ameliorates allergic airway inflammation by regulating activation of NLRP3 in mice |
title | Estrogen ameliorates allergic airway inflammation by regulating activation of NLRP3 in mice |
title_full | Estrogen ameliorates allergic airway inflammation by regulating activation of NLRP3 in mice |
title_fullStr | Estrogen ameliorates allergic airway inflammation by regulating activation of NLRP3 in mice |
title_full_unstemmed | Estrogen ameliorates allergic airway inflammation by regulating activation of NLRP3 in mice |
title_short | Estrogen ameliorates allergic airway inflammation by regulating activation of NLRP3 in mice |
title_sort | estrogen ameliorates allergic airway inflammation by regulating activation of nlrp3 in mice |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328879/ https://www.ncbi.nlm.nih.gov/pubmed/30373775 http://dx.doi.org/10.1042/BSR20181117 |
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