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Association of Growth Factors Genes with Miscarriage

BACKGROUND: The study was aimed to investigate the association of VEGFA gene polymorphic variants -2578C>A (rs699947) and -634G>C (rs2010963) and TGFB1 gene 915G>C (rs1800471) and gene expression level with miscarriage in the first trimester. METHODS: 288 women with different courses of pre...

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Autores principales: Marakhovskaya, Tatiana Alekseevna, Butenko, Elena Viktorovna, Kovalenko, Konstantin Alekseevich, Mashkina, Elena Vladimirovna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328980/
https://www.ncbi.nlm.nih.gov/pubmed/30746337
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author Marakhovskaya, Tatiana Alekseevna
Butenko, Elena Viktorovna
Kovalenko, Konstantin Alekseevich
Mashkina, Elena Vladimirovna
author_facet Marakhovskaya, Tatiana Alekseevna
Butenko, Elena Viktorovna
Kovalenko, Konstantin Alekseevich
Mashkina, Elena Vladimirovna
author_sort Marakhovskaya, Tatiana Alekseevna
collection PubMed
description BACKGROUND: The study was aimed to investigate the association of VEGFA gene polymorphic variants -2578C>A (rs699947) and -634G>C (rs2010963) and TGFB1 gene 915G>C (rs1800471) and gene expression level with miscarriage in the first trimester. METHODS: 288 women with different courses of pregnancy and 61 chorionic tissue samples were involved in case-control study. Allele-specific polymerase chain reaction in real time was used for genotyping. Next, gene-gene interactions were analyzed using the multifactor dimensionality reduction method. VEGFA and TGFB1 genes expression levels were determined by RT-PCR. RESULTS: It was found that SNP rs699947 was associated with the miscarriage risk change (p=0.05). The CC genotype was associated with reduced risk of abortion in the first trimester, and the CA genotype with increased risk. Genotypes VEGFA -2578CC/VEGFA -634CG, VEGFA -2578AA/VEGFA -634CG,and VEGFA -2578CC/ VEGFA -634CG/TGFB1 936CC were associated with lowered risk of miscarriage in the first trimester. mRNA level of TGFB1 was significantly higher in decidual tissue compared to chorionic tissue in normally progressing pregnancy (p=0.003). VEGFA gene expression level was directly correlated with the TGFB1 mRNA level (R=0.60; p=0.038). In pregnancy loss, an inverse relationship was observed (R=−0.76; p=0.028). CONCLUSION: The SNP rs699947 is associated with pregnancy loss in the first trimester. The MDR analysis data showed the significant relationship between VEGFA and TGFB1 genes in two-locus and three-locus models. A change in the ratio of the concentrations of growth factors can disrupt the processes of cell division, apoptosis and angiogenesis processes.
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spelling pubmed-63289802019-02-11 Association of Growth Factors Genes with Miscarriage Marakhovskaya, Tatiana Alekseevna Butenko, Elena Viktorovna Kovalenko, Konstantin Alekseevich Mashkina, Elena Vladimirovna J Reprod Infertil Original Article BACKGROUND: The study was aimed to investigate the association of VEGFA gene polymorphic variants -2578C>A (rs699947) and -634G>C (rs2010963) and TGFB1 gene 915G>C (rs1800471) and gene expression level with miscarriage in the first trimester. METHODS: 288 women with different courses of pregnancy and 61 chorionic tissue samples were involved in case-control study. Allele-specific polymerase chain reaction in real time was used for genotyping. Next, gene-gene interactions were analyzed using the multifactor dimensionality reduction method. VEGFA and TGFB1 genes expression levels were determined by RT-PCR. RESULTS: It was found that SNP rs699947 was associated with the miscarriage risk change (p=0.05). The CC genotype was associated with reduced risk of abortion in the first trimester, and the CA genotype with increased risk. Genotypes VEGFA -2578CC/VEGFA -634CG, VEGFA -2578AA/VEGFA -634CG,and VEGFA -2578CC/ VEGFA -634CG/TGFB1 936CC were associated with lowered risk of miscarriage in the first trimester. mRNA level of TGFB1 was significantly higher in decidual tissue compared to chorionic tissue in normally progressing pregnancy (p=0.003). VEGFA gene expression level was directly correlated with the TGFB1 mRNA level (R=0.60; p=0.038). In pregnancy loss, an inverse relationship was observed (R=−0.76; p=0.028). CONCLUSION: The SNP rs699947 is associated with pregnancy loss in the first trimester. The MDR analysis data showed the significant relationship between VEGFA and TGFB1 genes in two-locus and three-locus models. A change in the ratio of the concentrations of growth factors can disrupt the processes of cell division, apoptosis and angiogenesis processes. Avicenna Research Institute 2018 /pmc/articles/PMC6328980/ /pubmed/30746337 Text en Copyright© 2018, Avicenna Research Institute. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Marakhovskaya, Tatiana Alekseevna
Butenko, Elena Viktorovna
Kovalenko, Konstantin Alekseevich
Mashkina, Elena Vladimirovna
Association of Growth Factors Genes with Miscarriage
title Association of Growth Factors Genes with Miscarriage
title_full Association of Growth Factors Genes with Miscarriage
title_fullStr Association of Growth Factors Genes with Miscarriage
title_full_unstemmed Association of Growth Factors Genes with Miscarriage
title_short Association of Growth Factors Genes with Miscarriage
title_sort association of growth factors genes with miscarriage
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328980/
https://www.ncbi.nlm.nih.gov/pubmed/30746337
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