Cargando…
Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach
BACKGROUND: Serum haptoglobin (Hp) has been closely associated with cardio-cerebrovascular diseases. We investigated a metabolic profile associated with circulating Hp and carotid arterial functions via a targeted metabolomics approach to provide insight into potential mechanisms. METHODS: A total o...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329046/ https://www.ncbi.nlm.nih.gov/pubmed/30634984 http://dx.doi.org/10.1186/s12933-019-0808-2 |
_version_ | 1783386756601610240 |
---|---|
author | Wang, Shiyun Wang, Jie Zhang, Rong Zhao, Aihua Zheng, Xiaojiao Yan, Dandan Jiang, Feng Jia, Wei Hu, Cheng Jia, Weiping |
author_facet | Wang, Shiyun Wang, Jie Zhang, Rong Zhao, Aihua Zheng, Xiaojiao Yan, Dandan Jiang, Feng Jia, Wei Hu, Cheng Jia, Weiping |
author_sort | Wang, Shiyun |
collection | PubMed |
description | BACKGROUND: Serum haptoglobin (Hp) has been closely associated with cardio-cerebrovascular diseases. We investigated a metabolic profile associated with circulating Hp and carotid arterial functions via a targeted metabolomics approach to provide insight into potential mechanisms. METHODS: A total of 240 participants, including 120 patients with type 2 diabetes mellitus (T2DM) and 120 non-diabetes mellitus (non-DM) subjects were recruited in this study. Targeted metabolic profiles of serum metabolites were determined using an AbsoluteIDQ™ p180 Kit (BIOCRATES Life Sciences AG, Innsbruck, Austria). Ultrasound of the bilateral common carotid artery was used to measure intima-media thickness and inter-adventitial diameter. Serum Hp levels were tested by enzyme-linked immunosorbent assay. RESULTS: Serum Hp levels in T2DM patients and non-DM subjects were 103.40 (72.46, 131.99) mg/dL and 100.20 (53.99, 140.66) mg/dL, respectively. Significant differences of 19 metabolites and 17 metabolites were found among serum Hp tertiles in T2DM patients and non-DM subjects, respectively (P < 0.05). Of these, phosphatidylcholine acyl-alkyl C32:2 (PC ae C32:2) was the common metabolite observed in two populations, which was associated with the serum Hp groups and lipid traits (P < 0.05). Furthermore, the metabolite ratios of two acidic amino acids, including aspartate to PC ae C32:2 (Asp/PC ae C32:2) and glutamate to PC ae C32:2 (Glu/PC ae C32:2) were correlated with serum Hp, carotid arterial functions and other biochemical index in both populations significantly (P < 0.05). CONCLUSIONS: Targeted metabolomics analyses might provide a new insight into the potential mechanisms underlying the association between serum Hp and carotid arterial functions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12933-019-0808-2) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6329046 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-63290462019-01-16 Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach Wang, Shiyun Wang, Jie Zhang, Rong Zhao, Aihua Zheng, Xiaojiao Yan, Dandan Jiang, Feng Jia, Wei Hu, Cheng Jia, Weiping Cardiovasc Diabetol Original Investigation BACKGROUND: Serum haptoglobin (Hp) has been closely associated with cardio-cerebrovascular diseases. We investigated a metabolic profile associated with circulating Hp and carotid arterial functions via a targeted metabolomics approach to provide insight into potential mechanisms. METHODS: A total of 240 participants, including 120 patients with type 2 diabetes mellitus (T2DM) and 120 non-diabetes mellitus (non-DM) subjects were recruited in this study. Targeted metabolic profiles of serum metabolites were determined using an AbsoluteIDQ™ p180 Kit (BIOCRATES Life Sciences AG, Innsbruck, Austria). Ultrasound of the bilateral common carotid artery was used to measure intima-media thickness and inter-adventitial diameter. Serum Hp levels were tested by enzyme-linked immunosorbent assay. RESULTS: Serum Hp levels in T2DM patients and non-DM subjects were 103.40 (72.46, 131.99) mg/dL and 100.20 (53.99, 140.66) mg/dL, respectively. Significant differences of 19 metabolites and 17 metabolites were found among serum Hp tertiles in T2DM patients and non-DM subjects, respectively (P < 0.05). Of these, phosphatidylcholine acyl-alkyl C32:2 (PC ae C32:2) was the common metabolite observed in two populations, which was associated with the serum Hp groups and lipid traits (P < 0.05). Furthermore, the metabolite ratios of two acidic amino acids, including aspartate to PC ae C32:2 (Asp/PC ae C32:2) and glutamate to PC ae C32:2 (Glu/PC ae C32:2) were correlated with serum Hp, carotid arterial functions and other biochemical index in both populations significantly (P < 0.05). CONCLUSIONS: Targeted metabolomics analyses might provide a new insight into the potential mechanisms underlying the association between serum Hp and carotid arterial functions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12933-019-0808-2) contains supplementary material, which is available to authorized users. BioMed Central 2019-01-11 /pmc/articles/PMC6329046/ /pubmed/30634984 http://dx.doi.org/10.1186/s12933-019-0808-2 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Original Investigation Wang, Shiyun Wang, Jie Zhang, Rong Zhao, Aihua Zheng, Xiaojiao Yan, Dandan Jiang, Feng Jia, Wei Hu, Cheng Jia, Weiping Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach |
title | Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach |
title_full | Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach |
title_fullStr | Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach |
title_full_unstemmed | Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach |
title_short | Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach |
title_sort | association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach |
topic | Original Investigation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329046/ https://www.ncbi.nlm.nih.gov/pubmed/30634984 http://dx.doi.org/10.1186/s12933-019-0808-2 |
work_keys_str_mv | AT wangshiyun associationbetweenserumhaptoglobinandcarotidarterialfunctionsusefulnessofatargetedmetabolomicsapproach AT wangjie associationbetweenserumhaptoglobinandcarotidarterialfunctionsusefulnessofatargetedmetabolomicsapproach AT zhangrong associationbetweenserumhaptoglobinandcarotidarterialfunctionsusefulnessofatargetedmetabolomicsapproach AT zhaoaihua associationbetweenserumhaptoglobinandcarotidarterialfunctionsusefulnessofatargetedmetabolomicsapproach AT zhengxiaojiao associationbetweenserumhaptoglobinandcarotidarterialfunctionsusefulnessofatargetedmetabolomicsapproach AT yandandan associationbetweenserumhaptoglobinandcarotidarterialfunctionsusefulnessofatargetedmetabolomicsapproach AT jiangfeng associationbetweenserumhaptoglobinandcarotidarterialfunctionsusefulnessofatargetedmetabolomicsapproach AT jiawei associationbetweenserumhaptoglobinandcarotidarterialfunctionsusefulnessofatargetedmetabolomicsapproach AT hucheng associationbetweenserumhaptoglobinandcarotidarterialfunctionsusefulnessofatargetedmetabolomicsapproach AT jiaweiping associationbetweenserumhaptoglobinandcarotidarterialfunctionsusefulnessofatargetedmetabolomicsapproach |