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Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach

BACKGROUND: Serum haptoglobin (Hp) has been closely associated with cardio-cerebrovascular diseases. We investigated a metabolic profile associated with circulating Hp and carotid arterial functions via a targeted metabolomics approach to provide insight into potential mechanisms. METHODS: A total o...

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Autores principales: Wang, Shiyun, Wang, Jie, Zhang, Rong, Zhao, Aihua, Zheng, Xiaojiao, Yan, Dandan, Jiang, Feng, Jia, Wei, Hu, Cheng, Jia, Weiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329046/
https://www.ncbi.nlm.nih.gov/pubmed/30634984
http://dx.doi.org/10.1186/s12933-019-0808-2
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author Wang, Shiyun
Wang, Jie
Zhang, Rong
Zhao, Aihua
Zheng, Xiaojiao
Yan, Dandan
Jiang, Feng
Jia, Wei
Hu, Cheng
Jia, Weiping
author_facet Wang, Shiyun
Wang, Jie
Zhang, Rong
Zhao, Aihua
Zheng, Xiaojiao
Yan, Dandan
Jiang, Feng
Jia, Wei
Hu, Cheng
Jia, Weiping
author_sort Wang, Shiyun
collection PubMed
description BACKGROUND: Serum haptoglobin (Hp) has been closely associated with cardio-cerebrovascular diseases. We investigated a metabolic profile associated with circulating Hp and carotid arterial functions via a targeted metabolomics approach to provide insight into potential mechanisms. METHODS: A total of 240 participants, including 120 patients with type 2 diabetes mellitus (T2DM) and 120 non-diabetes mellitus (non-DM) subjects were recruited in this study. Targeted metabolic profiles of serum metabolites were determined using an AbsoluteIDQ™ p180 Kit (BIOCRATES Life Sciences AG, Innsbruck, Austria). Ultrasound of the bilateral common carotid artery was used to measure intima-media thickness and inter-adventitial diameter. Serum Hp levels were tested by enzyme-linked immunosorbent assay. RESULTS: Serum Hp levels in T2DM patients and non-DM subjects were 103.40 (72.46, 131.99) mg/dL and 100.20 (53.99, 140.66) mg/dL, respectively. Significant differences of 19 metabolites and 17 metabolites were found among serum Hp tertiles in T2DM patients and non-DM subjects, respectively (P < 0.05). Of these, phosphatidylcholine acyl-alkyl C32:2 (PC ae C32:2) was the common metabolite observed in two populations, which was associated with the serum Hp groups and lipid traits (P < 0.05). Furthermore, the metabolite ratios of two acidic amino acids, including aspartate to PC ae C32:2 (Asp/PC ae C32:2) and glutamate to PC ae C32:2 (Glu/PC ae C32:2) were correlated with serum Hp, carotid arterial functions and other biochemical index in both populations significantly (P < 0.05). CONCLUSIONS: Targeted metabolomics analyses might provide a new insight into the potential mechanisms underlying the association between serum Hp and carotid arterial functions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12933-019-0808-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-63290462019-01-16 Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach Wang, Shiyun Wang, Jie Zhang, Rong Zhao, Aihua Zheng, Xiaojiao Yan, Dandan Jiang, Feng Jia, Wei Hu, Cheng Jia, Weiping Cardiovasc Diabetol Original Investigation BACKGROUND: Serum haptoglobin (Hp) has been closely associated with cardio-cerebrovascular diseases. We investigated a metabolic profile associated with circulating Hp and carotid arterial functions via a targeted metabolomics approach to provide insight into potential mechanisms. METHODS: A total of 240 participants, including 120 patients with type 2 diabetes mellitus (T2DM) and 120 non-diabetes mellitus (non-DM) subjects were recruited in this study. Targeted metabolic profiles of serum metabolites were determined using an AbsoluteIDQ™ p180 Kit (BIOCRATES Life Sciences AG, Innsbruck, Austria). Ultrasound of the bilateral common carotid artery was used to measure intima-media thickness and inter-adventitial diameter. Serum Hp levels were tested by enzyme-linked immunosorbent assay. RESULTS: Serum Hp levels in T2DM patients and non-DM subjects were 103.40 (72.46, 131.99) mg/dL and 100.20 (53.99, 140.66) mg/dL, respectively. Significant differences of 19 metabolites and 17 metabolites were found among serum Hp tertiles in T2DM patients and non-DM subjects, respectively (P < 0.05). Of these, phosphatidylcholine acyl-alkyl C32:2 (PC ae C32:2) was the common metabolite observed in two populations, which was associated with the serum Hp groups and lipid traits (P < 0.05). Furthermore, the metabolite ratios of two acidic amino acids, including aspartate to PC ae C32:2 (Asp/PC ae C32:2) and glutamate to PC ae C32:2 (Glu/PC ae C32:2) were correlated with serum Hp, carotid arterial functions and other biochemical index in both populations significantly (P < 0.05). CONCLUSIONS: Targeted metabolomics analyses might provide a new insight into the potential mechanisms underlying the association between serum Hp and carotid arterial functions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12933-019-0808-2) contains supplementary material, which is available to authorized users. BioMed Central 2019-01-11 /pmc/articles/PMC6329046/ /pubmed/30634984 http://dx.doi.org/10.1186/s12933-019-0808-2 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Original Investigation
Wang, Shiyun
Wang, Jie
Zhang, Rong
Zhao, Aihua
Zheng, Xiaojiao
Yan, Dandan
Jiang, Feng
Jia, Wei
Hu, Cheng
Jia, Weiping
Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach
title Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach
title_full Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach
title_fullStr Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach
title_full_unstemmed Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach
title_short Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach
title_sort association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach
topic Original Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329046/
https://www.ncbi.nlm.nih.gov/pubmed/30634984
http://dx.doi.org/10.1186/s12933-019-0808-2
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