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Direct radiation-induced effects on dental hard tissue

BACKGROUND: Radiation caries is a complication of radiotherapy characterized by enamel erosion and dentin exposure. The mechanisms of characteristic radiation caries formation are not well-understood. The aim of this study was to evaluate the direct radiation-induced effects on dental hard tissue an...

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Autores principales: Lu, Hui, Zhao, Qi, Guo, Jiang, Zeng, Binghui, Yu, Xinlin, Yu, Dongsheng, Zhao, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329176/
https://www.ncbi.nlm.nih.gov/pubmed/30635005
http://dx.doi.org/10.1186/s13014-019-1208-1
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author Lu, Hui
Zhao, Qi
Guo, Jiang
Zeng, Binghui
Yu, Xinlin
Yu, Dongsheng
Zhao, Wei
author_facet Lu, Hui
Zhao, Qi
Guo, Jiang
Zeng, Binghui
Yu, Xinlin
Yu, Dongsheng
Zhao, Wei
author_sort Lu, Hui
collection PubMed
description BACKGROUND: Radiation caries is a complication of radiotherapy characterized by enamel erosion and dentin exposure. The mechanisms of characteristic radiation caries formation are not well-understood. The aim of this study was to evaluate the direct radiation-induced effects on dental hard tissue and investigate their role in the formation of radiation caries. METHODS: Sixty non-carious third molars were divided into three groups (n = 20), which would be exposed to 0 Gy, 30 Gy, and 60 Gy radiation, respectively. After radiation, microhardness and elastic modulus were measured at four depths by means of a Vickers microhardness tester and atomic force microscopy (AFM). The microstructure was observed by scanning electron microscopy (SEM). X-ray diffraction and Raman microspectroscopy were used to determine crystal properties and protein/mineral (2931/960 cm(− 1)) ratios. RESULTS: A statistically significant decrease in microhardness and elastic modulus values 50 μm from the dentino-enamel junction (DEJ) in enamel was revealed in the 30-Gy and 60-Gy groups. With the increasing dose, destruction of interprismatic substance and fissures at the DEJ-adjacent region were found. A greater reduction of crystallinity was revealed in enamel compared with dentin. Raman spectroscopic analysis showed a slight increase of the protein/mineral ratio for enamel following accumulated radiation, while the protein/mineral ratio for dentin was decreased. CONCLUSIONS: Radiation could directly alter the mechanical properties, micro-morphology, crystal properties, and chemical composition of dental hard tissue. The early destruction of DEJ-adjacent enamel, combined with decreased crystallinity of enamel under radiation exposure, may be related to the formation of characteristic radiation caries.
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spelling pubmed-63291762019-01-16 Direct radiation-induced effects on dental hard tissue Lu, Hui Zhao, Qi Guo, Jiang Zeng, Binghui Yu, Xinlin Yu, Dongsheng Zhao, Wei Radiat Oncol Research BACKGROUND: Radiation caries is a complication of radiotherapy characterized by enamel erosion and dentin exposure. The mechanisms of characteristic radiation caries formation are not well-understood. The aim of this study was to evaluate the direct radiation-induced effects on dental hard tissue and investigate their role in the formation of radiation caries. METHODS: Sixty non-carious third molars were divided into three groups (n = 20), which would be exposed to 0 Gy, 30 Gy, and 60 Gy radiation, respectively. After radiation, microhardness and elastic modulus were measured at four depths by means of a Vickers microhardness tester and atomic force microscopy (AFM). The microstructure was observed by scanning electron microscopy (SEM). X-ray diffraction and Raman microspectroscopy were used to determine crystal properties and protein/mineral (2931/960 cm(− 1)) ratios. RESULTS: A statistically significant decrease in microhardness and elastic modulus values 50 μm from the dentino-enamel junction (DEJ) in enamel was revealed in the 30-Gy and 60-Gy groups. With the increasing dose, destruction of interprismatic substance and fissures at the DEJ-adjacent region were found. A greater reduction of crystallinity was revealed in enamel compared with dentin. Raman spectroscopic analysis showed a slight increase of the protein/mineral ratio for enamel following accumulated radiation, while the protein/mineral ratio for dentin was decreased. CONCLUSIONS: Radiation could directly alter the mechanical properties, micro-morphology, crystal properties, and chemical composition of dental hard tissue. The early destruction of DEJ-adjacent enamel, combined with decreased crystallinity of enamel under radiation exposure, may be related to the formation of characteristic radiation caries. BioMed Central 2019-01-11 /pmc/articles/PMC6329176/ /pubmed/30635005 http://dx.doi.org/10.1186/s13014-019-1208-1 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Lu, Hui
Zhao, Qi
Guo, Jiang
Zeng, Binghui
Yu, Xinlin
Yu, Dongsheng
Zhao, Wei
Direct radiation-induced effects on dental hard tissue
title Direct radiation-induced effects on dental hard tissue
title_full Direct radiation-induced effects on dental hard tissue
title_fullStr Direct radiation-induced effects on dental hard tissue
title_full_unstemmed Direct radiation-induced effects on dental hard tissue
title_short Direct radiation-induced effects on dental hard tissue
title_sort direct radiation-induced effects on dental hard tissue
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329176/
https://www.ncbi.nlm.nih.gov/pubmed/30635005
http://dx.doi.org/10.1186/s13014-019-1208-1
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