Cargando…

Assessment of dental caries and salivary nitric oxide levels in children with dyspepsia

BACKGROUND: The increase in nitric oxide (NO) levels in the oral cavity and saliva have been associated with various oral diseases; however, the gastro-salivary interaction of NO remains controversial. Thus, the aim of this study was to determine and compare salivary NO levels of dyspeptic and non-d...

Descripción completa

Detalles Bibliográficos
Autores principales: Aksit-Bicak, Damla, Emekli-Alturfan, Ebru, Ustundag, Unsal Veli, Akyuz, Serap
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329182/
https://www.ncbi.nlm.nih.gov/pubmed/30634964
http://dx.doi.org/10.1186/s12903-018-0707-z
_version_ 1783386788630364160
author Aksit-Bicak, Damla
Emekli-Alturfan, Ebru
Ustundag, Unsal Veli
Akyuz, Serap
author_facet Aksit-Bicak, Damla
Emekli-Alturfan, Ebru
Ustundag, Unsal Veli
Akyuz, Serap
author_sort Aksit-Bicak, Damla
collection PubMed
description BACKGROUND: The increase in nitric oxide (NO) levels in the oral cavity and saliva have been associated with various oral diseases; however, the gastro-salivary interaction of NO remains controversial. Thus, the aim of this study was to determine and compare salivary NO levels of dyspeptic and non-dyspeptic healthy children and to conduct an evaluation of its association with dental caries. METHODS: Seventy children with dyspepsia (dyspeptic group) and 30 children without any gastrointestinal complaints (control group) were included in the study. Two biopsies from the gastric tissues were collected from dyspeptic children for histopathologic examination. Oral examination involved the assessment of dental caries, gingival index, plaque index, buffering capacity, salivary flow rate and pH. Salivary Streptococcus mutans (S. mutans) and Lactobacilli sp. counts were performed by commercial kits. For the comparison of the normal distribution between dyspeptic and control groups, Student t-test and for the comparison of the non-normal distribution, Kruskal-Wallis and Mann-Whitney-U tests were used. Chi-square test was used for comparison of qualitative data and the Pearson correlation test was used to evaluate the association between certain variables. Significance was assessed at p < 0.05 level. RESULTS: Helicobacter pylori (H.pylori) were found in gastric biopsies of 84.2% (59/70) of the dyspeptic children. While the mean salivary NO values did not differ significantly between gastric H.pylori positive, negative and control groups, the salivary NO level of the dyspeptic group (213.7 ± 51.68 μmol/dL) was found to be significantly higher than the control group (185.7 ± 16.66 μmol/dL). No significant relationship was found between the mean salivary NO values, DMFT/dmft numbers and other oral parameters. CONCLUSIONS: The association of dental caries and salivary NO levels could not be considered specific in the current study. Although there were no statistically significant differences between salivary NO levels of gastric H.pylori positive, gastric H.pylori negative and control groups, greater salivary NO levels among dyspeptic children compared with the control group demonstrated that the concentration of NO in the saliva could be used as a biological marker in dyspepsia, which could lead to the improvement of more specified, uncomplicated and susceptible methods for analysis.
format Online
Article
Text
id pubmed-6329182
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-63291822019-01-16 Assessment of dental caries and salivary nitric oxide levels in children with dyspepsia Aksit-Bicak, Damla Emekli-Alturfan, Ebru Ustundag, Unsal Veli Akyuz, Serap BMC Oral Health Research Article BACKGROUND: The increase in nitric oxide (NO) levels in the oral cavity and saliva have been associated with various oral diseases; however, the gastro-salivary interaction of NO remains controversial. Thus, the aim of this study was to determine and compare salivary NO levels of dyspeptic and non-dyspeptic healthy children and to conduct an evaluation of its association with dental caries. METHODS: Seventy children with dyspepsia (dyspeptic group) and 30 children without any gastrointestinal complaints (control group) were included in the study. Two biopsies from the gastric tissues were collected from dyspeptic children for histopathologic examination. Oral examination involved the assessment of dental caries, gingival index, plaque index, buffering capacity, salivary flow rate and pH. Salivary Streptococcus mutans (S. mutans) and Lactobacilli sp. counts were performed by commercial kits. For the comparison of the normal distribution between dyspeptic and control groups, Student t-test and for the comparison of the non-normal distribution, Kruskal-Wallis and Mann-Whitney-U tests were used. Chi-square test was used for comparison of qualitative data and the Pearson correlation test was used to evaluate the association between certain variables. Significance was assessed at p < 0.05 level. RESULTS: Helicobacter pylori (H.pylori) were found in gastric biopsies of 84.2% (59/70) of the dyspeptic children. While the mean salivary NO values did not differ significantly between gastric H.pylori positive, negative and control groups, the salivary NO level of the dyspeptic group (213.7 ± 51.68 μmol/dL) was found to be significantly higher than the control group (185.7 ± 16.66 μmol/dL). No significant relationship was found between the mean salivary NO values, DMFT/dmft numbers and other oral parameters. CONCLUSIONS: The association of dental caries and salivary NO levels could not be considered specific in the current study. Although there were no statistically significant differences between salivary NO levels of gastric H.pylori positive, gastric H.pylori negative and control groups, greater salivary NO levels among dyspeptic children compared with the control group demonstrated that the concentration of NO in the saliva could be used as a biological marker in dyspepsia, which could lead to the improvement of more specified, uncomplicated and susceptible methods for analysis. BioMed Central 2019-01-11 /pmc/articles/PMC6329182/ /pubmed/30634964 http://dx.doi.org/10.1186/s12903-018-0707-z Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Aksit-Bicak, Damla
Emekli-Alturfan, Ebru
Ustundag, Unsal Veli
Akyuz, Serap
Assessment of dental caries and salivary nitric oxide levels in children with dyspepsia
title Assessment of dental caries and salivary nitric oxide levels in children with dyspepsia
title_full Assessment of dental caries and salivary nitric oxide levels in children with dyspepsia
title_fullStr Assessment of dental caries and salivary nitric oxide levels in children with dyspepsia
title_full_unstemmed Assessment of dental caries and salivary nitric oxide levels in children with dyspepsia
title_short Assessment of dental caries and salivary nitric oxide levels in children with dyspepsia
title_sort assessment of dental caries and salivary nitric oxide levels in children with dyspepsia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329182/
https://www.ncbi.nlm.nih.gov/pubmed/30634964
http://dx.doi.org/10.1186/s12903-018-0707-z
work_keys_str_mv AT aksitbicakdamla assessmentofdentalcariesandsalivarynitricoxidelevelsinchildrenwithdyspepsia
AT emeklialturfanebru assessmentofdentalcariesandsalivarynitricoxidelevelsinchildrenwithdyspepsia
AT ustundagunsalveli assessmentofdentalcariesandsalivarynitricoxidelevelsinchildrenwithdyspepsia
AT akyuzserap assessmentofdentalcariesandsalivarynitricoxidelevelsinchildrenwithdyspepsia