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miR-302 cluster inhibits angiogenesis and growth of K562 leukemia cells by targeting VEGFA
BACKGROUND: miR-302 cluster has been reported as a tumor suppressor in many human cancers; yet, its function in chronic myeloid leukemia (CML) tumorigenesis remains largely unclear. The study was aimed to explore the functional roles of miR-302 cluster in CML progression. MATERIALS AND METHODS: Quan...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329480/ https://www.ncbi.nlm.nih.gov/pubmed/30662269 http://dx.doi.org/10.2147/OTT.S190146 |
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author | Cao, Jiang Li, Li Han, Xiao Cheng, Hai Chen, Wei Qi, Kunming Chen, Chong Wu, Qingyun Niu, Mingshan Zeng, Lingyu Xu, Kailin |
author_facet | Cao, Jiang Li, Li Han, Xiao Cheng, Hai Chen, Wei Qi, Kunming Chen, Chong Wu, Qingyun Niu, Mingshan Zeng, Lingyu Xu, Kailin |
author_sort | Cao, Jiang |
collection | PubMed |
description | BACKGROUND: miR-302 cluster has been reported as a tumor suppressor in many human cancers; yet, its function in chronic myeloid leukemia (CML) tumorigenesis remains largely unclear. The study was aimed to explore the functional roles of miR-302 cluster in CML progression. MATERIALS AND METHODS: Quantitative reverse transcriptase PCR and Western blot were performed to evaluate miR-302 cluster and vascular endothelial growth factor A (VEGFA) expression levels. Cell Counting Kit-8 assay, colony formation assay and human umbilical vein endothelial cell line capillary tube formation were used to determine the influence of miR-302 cluster on the growth and angiogenesis of K562 cells, respectively. Luciferase reporter assay was employed to confirm the direct target interaction between miR-302 cluster and VEGFA. RESULTS: This study demonstrated that miR-302 cluster was frequently downregulated in CML samples and cell lines and high level of miR-302 cluster was significantly associated with good prognosis of CML patients. Compared with miRNA negative control, miR-302 cluster mimics obviously suppressed cell growth, colony formation and angiogenesis. Further studies revealed that VEGFA was a direct target gene of miR-302 cluster. Moreover, overexpression of VEGFA dramatically abated the inhibition of miR-302 cluster on cell growth and angiogenesis. CONCLUSION: The present study, for the first time, identified miR-302 cluster as a tumor suppressor, and overexpression of miR-302 cluster inhibited growth and angiogenesis in K562 cells. miR-302 cluster may be a potential therapeutic target in CML to develop the adjuvant antiangiogenic therapy based on VEGFA. |
format | Online Article Text |
id | pubmed-6329480 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-63294802019-01-18 miR-302 cluster inhibits angiogenesis and growth of K562 leukemia cells by targeting VEGFA Cao, Jiang Li, Li Han, Xiao Cheng, Hai Chen, Wei Qi, Kunming Chen, Chong Wu, Qingyun Niu, Mingshan Zeng, Lingyu Xu, Kailin Onco Targets Ther Original Research BACKGROUND: miR-302 cluster has been reported as a tumor suppressor in many human cancers; yet, its function in chronic myeloid leukemia (CML) tumorigenesis remains largely unclear. The study was aimed to explore the functional roles of miR-302 cluster in CML progression. MATERIALS AND METHODS: Quantitative reverse transcriptase PCR and Western blot were performed to evaluate miR-302 cluster and vascular endothelial growth factor A (VEGFA) expression levels. Cell Counting Kit-8 assay, colony formation assay and human umbilical vein endothelial cell line capillary tube formation were used to determine the influence of miR-302 cluster on the growth and angiogenesis of K562 cells, respectively. Luciferase reporter assay was employed to confirm the direct target interaction between miR-302 cluster and VEGFA. RESULTS: This study demonstrated that miR-302 cluster was frequently downregulated in CML samples and cell lines and high level of miR-302 cluster was significantly associated with good prognosis of CML patients. Compared with miRNA negative control, miR-302 cluster mimics obviously suppressed cell growth, colony formation and angiogenesis. Further studies revealed that VEGFA was a direct target gene of miR-302 cluster. Moreover, overexpression of VEGFA dramatically abated the inhibition of miR-302 cluster on cell growth and angiogenesis. CONCLUSION: The present study, for the first time, identified miR-302 cluster as a tumor suppressor, and overexpression of miR-302 cluster inhibited growth and angiogenesis in K562 cells. miR-302 cluster may be a potential therapeutic target in CML to develop the adjuvant antiangiogenic therapy based on VEGFA. Dove Medical Press 2019-01-08 /pmc/articles/PMC6329480/ /pubmed/30662269 http://dx.doi.org/10.2147/OTT.S190146 Text en © 2019 Cao et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Cao, Jiang Li, Li Han, Xiao Cheng, Hai Chen, Wei Qi, Kunming Chen, Chong Wu, Qingyun Niu, Mingshan Zeng, Lingyu Xu, Kailin miR-302 cluster inhibits angiogenesis and growth of K562 leukemia cells by targeting VEGFA |
title | miR-302 cluster inhibits angiogenesis and growth of K562 leukemia cells by targeting VEGFA |
title_full | miR-302 cluster inhibits angiogenesis and growth of K562 leukemia cells by targeting VEGFA |
title_fullStr | miR-302 cluster inhibits angiogenesis and growth of K562 leukemia cells by targeting VEGFA |
title_full_unstemmed | miR-302 cluster inhibits angiogenesis and growth of K562 leukemia cells by targeting VEGFA |
title_short | miR-302 cluster inhibits angiogenesis and growth of K562 leukemia cells by targeting VEGFA |
title_sort | mir-302 cluster inhibits angiogenesis and growth of k562 leukemia cells by targeting vegfa |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329480/ https://www.ncbi.nlm.nih.gov/pubmed/30662269 http://dx.doi.org/10.2147/OTT.S190146 |
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