Cargando…
A gene expression map of shoot domains reveals regulatory mechanisms
Gene regulatory networks control development via domain-specific gene expression. In seed plants, self-renewing stem cells located in the shoot apical meristem (SAM) produce leaves from the SAM peripheral zone. After initiation, leaves develop polarity patterns to form a planar shape. Here we compar...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329838/ https://www.ncbi.nlm.nih.gov/pubmed/30635575 http://dx.doi.org/10.1038/s41467-018-08083-z |
_version_ | 1783386880121765888 |
---|---|
author | Tian, Caihuan Wang, Ying Yu, Haopeng He, Jun Wang, Jin Shi, Bihai Du, Qingwei Provart, Nicholas J. Meyerowitz, Elliot M. Jiao, Yuling |
author_facet | Tian, Caihuan Wang, Ying Yu, Haopeng He, Jun Wang, Jin Shi, Bihai Du, Qingwei Provart, Nicholas J. Meyerowitz, Elliot M. Jiao, Yuling |
author_sort | Tian, Caihuan |
collection | PubMed |
description | Gene regulatory networks control development via domain-specific gene expression. In seed plants, self-renewing stem cells located in the shoot apical meristem (SAM) produce leaves from the SAM peripheral zone. After initiation, leaves develop polarity patterns to form a planar shape. Here we compare translating RNAs among SAM and leaf domains. Using translating ribosome affinity purification and RNA sequencing to quantify gene expression in target domains, we generate a domain-specific translatome map covering representative vegetative stage SAM and leaf domains. We discuss the predicted cellular functions of these domains and provide evidence that dome seemingly unrelated domains, utilize common regulatory modules. Experimental follow up shows that the RABBIT EARS and HANABA TARANU transcription factors have roles in axillary meristem initiation. This dataset provides a community resource for further study of shoot development and response to internal and environmental signals. |
format | Online Article Text |
id | pubmed-6329838 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-63298382019-01-15 A gene expression map of shoot domains reveals regulatory mechanisms Tian, Caihuan Wang, Ying Yu, Haopeng He, Jun Wang, Jin Shi, Bihai Du, Qingwei Provart, Nicholas J. Meyerowitz, Elliot M. Jiao, Yuling Nat Commun Article Gene regulatory networks control development via domain-specific gene expression. In seed plants, self-renewing stem cells located in the shoot apical meristem (SAM) produce leaves from the SAM peripheral zone. After initiation, leaves develop polarity patterns to form a planar shape. Here we compare translating RNAs among SAM and leaf domains. Using translating ribosome affinity purification and RNA sequencing to quantify gene expression in target domains, we generate a domain-specific translatome map covering representative vegetative stage SAM and leaf domains. We discuss the predicted cellular functions of these domains and provide evidence that dome seemingly unrelated domains, utilize common regulatory modules. Experimental follow up shows that the RABBIT EARS and HANABA TARANU transcription factors have roles in axillary meristem initiation. This dataset provides a community resource for further study of shoot development and response to internal and environmental signals. Nature Publishing Group UK 2019-01-11 /pmc/articles/PMC6329838/ /pubmed/30635575 http://dx.doi.org/10.1038/s41467-018-08083-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Tian, Caihuan Wang, Ying Yu, Haopeng He, Jun Wang, Jin Shi, Bihai Du, Qingwei Provart, Nicholas J. Meyerowitz, Elliot M. Jiao, Yuling A gene expression map of shoot domains reveals regulatory mechanisms |
title | A gene expression map of shoot domains reveals regulatory mechanisms |
title_full | A gene expression map of shoot domains reveals regulatory mechanisms |
title_fullStr | A gene expression map of shoot domains reveals regulatory mechanisms |
title_full_unstemmed | A gene expression map of shoot domains reveals regulatory mechanisms |
title_short | A gene expression map of shoot domains reveals regulatory mechanisms |
title_sort | gene expression map of shoot domains reveals regulatory mechanisms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329838/ https://www.ncbi.nlm.nih.gov/pubmed/30635575 http://dx.doi.org/10.1038/s41467-018-08083-z |
work_keys_str_mv | AT tiancaihuan ageneexpressionmapofshootdomainsrevealsregulatorymechanisms AT wangying ageneexpressionmapofshootdomainsrevealsregulatorymechanisms AT yuhaopeng ageneexpressionmapofshootdomainsrevealsregulatorymechanisms AT hejun ageneexpressionmapofshootdomainsrevealsregulatorymechanisms AT wangjin ageneexpressionmapofshootdomainsrevealsregulatorymechanisms AT shibihai ageneexpressionmapofshootdomainsrevealsregulatorymechanisms AT duqingwei ageneexpressionmapofshootdomainsrevealsregulatorymechanisms AT provartnicholasj ageneexpressionmapofshootdomainsrevealsregulatorymechanisms AT meyerowitzelliotm ageneexpressionmapofshootdomainsrevealsregulatorymechanisms AT jiaoyuling ageneexpressionmapofshootdomainsrevealsregulatorymechanisms AT tiancaihuan geneexpressionmapofshootdomainsrevealsregulatorymechanisms AT wangying geneexpressionmapofshootdomainsrevealsregulatorymechanisms AT yuhaopeng geneexpressionmapofshootdomainsrevealsregulatorymechanisms AT hejun geneexpressionmapofshootdomainsrevealsregulatorymechanisms AT wangjin geneexpressionmapofshootdomainsrevealsregulatorymechanisms AT shibihai geneexpressionmapofshootdomainsrevealsregulatorymechanisms AT duqingwei geneexpressionmapofshootdomainsrevealsregulatorymechanisms AT provartnicholasj geneexpressionmapofshootdomainsrevealsregulatorymechanisms AT meyerowitzelliotm geneexpressionmapofshootdomainsrevealsregulatorymechanisms AT jiaoyuling geneexpressionmapofshootdomainsrevealsregulatorymechanisms |