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Didymin improves UV irradiation resistance in C. elegans
Didymin, a type of flavono-o-glycoside compound naturally present in citrus fruits, has been reported to be an effective anticancer agent. However, its effects on stress resistance are unclear. In this study, we treated Caenorhabditis elegans with didymin at several concentrations. We found that did...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330030/ https://www.ncbi.nlm.nih.gov/pubmed/30643686 http://dx.doi.org/10.7717/peerj.6218 |
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author | Zhou, Lin Wang, Lu Zhang, Jialing Li, Jiahe Bai, Shuju Ma, Junfeng Fu, Xueqi |
author_facet | Zhou, Lin Wang, Lu Zhang, Jialing Li, Jiahe Bai, Shuju Ma, Junfeng Fu, Xueqi |
author_sort | Zhou, Lin |
collection | PubMed |
description | Didymin, a type of flavono-o-glycoside compound naturally present in citrus fruits, has been reported to be an effective anticancer agent. However, its effects on stress resistance are unclear. In this study, we treated Caenorhabditis elegans with didymin at several concentrations. We found that didymin reduced the effects of UV stressor on nematodes by decreasing reactive oxygen species levels and increasing superoxide dismutase (SOD) activity. Furthermore, we found that specific didymin-treated mutant nematodes daf-16(mu86) & daf-2(e1370), daf-16(mu86), akt-1(ok525), akt-2(ok393), and age-1(hx546) were susceptible to UV irradiation, whereas daf-2(e1371) was resistant to UV irradiation. In addition, we found that didymin not only promoted DAF-16 to transfer from cytoplasm to nucleus, but also increased both protein and mRNA expression levels of SOD-3 and HSP-16.2 after UV irradiation. Our results show that didymin affects UV irradiation resistance and it may act on daf-2 to regulate downstream genes through the insulin/IGF-1-like signaling pathway. |
format | Online Article Text |
id | pubmed-6330030 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63300302019-01-14 Didymin improves UV irradiation resistance in C. elegans Zhou, Lin Wang, Lu Zhang, Jialing Li, Jiahe Bai, Shuju Ma, Junfeng Fu, Xueqi PeerJ Biochemistry Didymin, a type of flavono-o-glycoside compound naturally present in citrus fruits, has been reported to be an effective anticancer agent. However, its effects on stress resistance are unclear. In this study, we treated Caenorhabditis elegans with didymin at several concentrations. We found that didymin reduced the effects of UV stressor on nematodes by decreasing reactive oxygen species levels and increasing superoxide dismutase (SOD) activity. Furthermore, we found that specific didymin-treated mutant nematodes daf-16(mu86) & daf-2(e1370), daf-16(mu86), akt-1(ok525), akt-2(ok393), and age-1(hx546) were susceptible to UV irradiation, whereas daf-2(e1371) was resistant to UV irradiation. In addition, we found that didymin not only promoted DAF-16 to transfer from cytoplasm to nucleus, but also increased both protein and mRNA expression levels of SOD-3 and HSP-16.2 after UV irradiation. Our results show that didymin affects UV irradiation resistance and it may act on daf-2 to regulate downstream genes through the insulin/IGF-1-like signaling pathway. PeerJ Inc. 2019-01-09 /pmc/articles/PMC6330030/ /pubmed/30643686 http://dx.doi.org/10.7717/peerj.6218 Text en © 2019 Zhou et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Biochemistry Zhou, Lin Wang, Lu Zhang, Jialing Li, Jiahe Bai, Shuju Ma, Junfeng Fu, Xueqi Didymin improves UV irradiation resistance in C. elegans |
title | Didymin improves UV irradiation resistance in C. elegans |
title_full | Didymin improves UV irradiation resistance in C. elegans |
title_fullStr | Didymin improves UV irradiation resistance in C. elegans |
title_full_unstemmed | Didymin improves UV irradiation resistance in C. elegans |
title_short | Didymin improves UV irradiation resistance in C. elegans |
title_sort | didymin improves uv irradiation resistance in c. elegans |
topic | Biochemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330030/ https://www.ncbi.nlm.nih.gov/pubmed/30643686 http://dx.doi.org/10.7717/peerj.6218 |
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