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Whole-genome comparisons of Penicillium spp. reveals secondary metabolic gene clusters and candidate genes associated with fungal aggressiveness during apple fruit decay

Blue mold is a postharvest rot of pomaceous fruits caused by Penicillium expansum and a number of other Penicillium species. The genome of the highly aggressive P. expansum strain R19 was re-sequenced and analyzed together with the genome of the less aggressive P. solitum strain RS1. Whole genome sc...

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Autores principales: Wu, Guangxi, Jurick II, Wayne M., Lichtner, Franz J., Peng, Hui, Yin, Guohua, Gaskins, Verneta L., Yin, Yanbin, Hua, Sui-Sheng, Peter, Kari A., Bennett, Joan W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330040/
https://www.ncbi.nlm.nih.gov/pubmed/30643697
http://dx.doi.org/10.7717/peerj.6170
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author Wu, Guangxi
Jurick II, Wayne M.
Lichtner, Franz J.
Peng, Hui
Yin, Guohua
Gaskins, Verneta L.
Yin, Yanbin
Hua, Sui-Sheng
Peter, Kari A.
Bennett, Joan W.
author_facet Wu, Guangxi
Jurick II, Wayne M.
Lichtner, Franz J.
Peng, Hui
Yin, Guohua
Gaskins, Verneta L.
Yin, Yanbin
Hua, Sui-Sheng
Peter, Kari A.
Bennett, Joan W.
author_sort Wu, Guangxi
collection PubMed
description Blue mold is a postharvest rot of pomaceous fruits caused by Penicillium expansum and a number of other Penicillium species. The genome of the highly aggressive P. expansum strain R19 was re-sequenced and analyzed together with the genome of the less aggressive P. solitum strain RS1. Whole genome scale similarities and differences were examined. A phylogenetic analysis of P. expansum, P. solitum, and several closely related Penicillium species revealed that the two pathogens isolated from decayed apple with blue mold symptoms are not each other’s closest relatives. Among a total of 10,560 and 10,672 protein coding sequences respectively, a comparative genomics analysis revealed 41 genes in P. expansum R19 and 43 genes in P. solitum RS1 that are unique to these two species. These genes may be associated with pome fruit–fungal interactions, subsequent decay processes, and mycotoxin accumulation. An intact patulin gene cluster consisting of 15 biosynthetic genes was identified in the patulin producing P. expansum strain R19, while only a remnant, seven-gene cluster was identified in the patulin-deficient P. solitum strain. However, P. solitum contained a large number of additional secondary metabolite gene clusters, indicating that this species has the potential capacity to produce an array of known as well as not-yet-identified products of possible toxicological or biotechnological interest.
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spelling pubmed-63300402019-01-14 Whole-genome comparisons of Penicillium spp. reveals secondary metabolic gene clusters and candidate genes associated with fungal aggressiveness during apple fruit decay Wu, Guangxi Jurick II, Wayne M. Lichtner, Franz J. Peng, Hui Yin, Guohua Gaskins, Verneta L. Yin, Yanbin Hua, Sui-Sheng Peter, Kari A. Bennett, Joan W. PeerJ Agricultural Science Blue mold is a postharvest rot of pomaceous fruits caused by Penicillium expansum and a number of other Penicillium species. The genome of the highly aggressive P. expansum strain R19 was re-sequenced and analyzed together with the genome of the less aggressive P. solitum strain RS1. Whole genome scale similarities and differences were examined. A phylogenetic analysis of P. expansum, P. solitum, and several closely related Penicillium species revealed that the two pathogens isolated from decayed apple with blue mold symptoms are not each other’s closest relatives. Among a total of 10,560 and 10,672 protein coding sequences respectively, a comparative genomics analysis revealed 41 genes in P. expansum R19 and 43 genes in P. solitum RS1 that are unique to these two species. These genes may be associated with pome fruit–fungal interactions, subsequent decay processes, and mycotoxin accumulation. An intact patulin gene cluster consisting of 15 biosynthetic genes was identified in the patulin producing P. expansum strain R19, while only a remnant, seven-gene cluster was identified in the patulin-deficient P. solitum strain. However, P. solitum contained a large number of additional secondary metabolite gene clusters, indicating that this species has the potential capacity to produce an array of known as well as not-yet-identified products of possible toxicological or biotechnological interest. PeerJ Inc. 2019-01-09 /pmc/articles/PMC6330040/ /pubmed/30643697 http://dx.doi.org/10.7717/peerj.6170 Text en ©2019 Wu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Wu, Guangxi
Jurick II, Wayne M.
Lichtner, Franz J.
Peng, Hui
Yin, Guohua
Gaskins, Verneta L.
Yin, Yanbin
Hua, Sui-Sheng
Peter, Kari A.
Bennett, Joan W.
Whole-genome comparisons of Penicillium spp. reveals secondary metabolic gene clusters and candidate genes associated with fungal aggressiveness during apple fruit decay
title Whole-genome comparisons of Penicillium spp. reveals secondary metabolic gene clusters and candidate genes associated with fungal aggressiveness during apple fruit decay
title_full Whole-genome comparisons of Penicillium spp. reveals secondary metabolic gene clusters and candidate genes associated with fungal aggressiveness during apple fruit decay
title_fullStr Whole-genome comparisons of Penicillium spp. reveals secondary metabolic gene clusters and candidate genes associated with fungal aggressiveness during apple fruit decay
title_full_unstemmed Whole-genome comparisons of Penicillium spp. reveals secondary metabolic gene clusters and candidate genes associated with fungal aggressiveness during apple fruit decay
title_short Whole-genome comparisons of Penicillium spp. reveals secondary metabolic gene clusters and candidate genes associated with fungal aggressiveness during apple fruit decay
title_sort whole-genome comparisons of penicillium spp. reveals secondary metabolic gene clusters and candidate genes associated with fungal aggressiveness during apple fruit decay
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330040/
https://www.ncbi.nlm.nih.gov/pubmed/30643697
http://dx.doi.org/10.7717/peerj.6170
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