Cargando…
Time- and Ventricular-Specific Expression Profiles of Genes Encoding Z-Disk Proteins in Pressure Overload Model of Left Ventricular Hypertrophy
Mechanotransduction is an essential mechanism of transforming external mechanical stimulus to biochemical response. In cardiomyocytes mechanotransduction plays an important role in contraction, stretch sensing and homeostasis regulation. One of the major mechanosensitive area in cardiomyocytes, the...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330284/ https://www.ncbi.nlm.nih.gov/pubmed/30666270 http://dx.doi.org/10.3389/fgene.2018.00684 |
_version_ | 1783386937796591616 |
---|---|
author | Knyazeva, Anastasia Krutikov, Alexander Golovkin, Alexey Mishanin, Alexander Pavlov, Georgii Smolina, Natalia Hushkina, Anastasia Sejersen, Thomas Sjoberg, Gunnar Galagudza, Mikhail Kostareva, Anna |
author_facet | Knyazeva, Anastasia Krutikov, Alexander Golovkin, Alexey Mishanin, Alexander Pavlov, Georgii Smolina, Natalia Hushkina, Anastasia Sejersen, Thomas Sjoberg, Gunnar Galagudza, Mikhail Kostareva, Anna |
author_sort | Knyazeva, Anastasia |
collection | PubMed |
description | Mechanotransduction is an essential mechanism of transforming external mechanical stimulus to biochemical response. In cardiomyocytes mechanotransduction plays an important role in contraction, stretch sensing and homeostasis regulation. One of the major mechanosensitive area in cardiomyocytes, the Z-disk, consists of numbers of structural and signaling proteins, that may undergo conformational or gene expression changes under pathological stress conditions. In present study we examined a rat model of pressure overload cardiac hypertrophy validated by echocardiographic and histopathological examinations. We revealed, that during hypertrophy progression expression of several genes encoding Z-disk proteins (Actn2, Ldb3, Cmya5, Nebl) is different at early and late points of cardiac remodeling. Moreover, expression patterns of several genes are opposite in myocardium of overloaded left ventricle and hemodynamically unaffected right ventricle, and expression profiles in interventricular septum are more similar to right ventricle. Additionally, we revealed inconsistencies between mRNA and protein level changes of Actn2, one of the major structural Z-disk element. All these findings point out, that investigated Z-disk proteins participate in pathological stress adaptation through undergoing the gene expression changes, and suggest the novel important role of hypertrophic response modulation during different stages of cardiac remodeling. |
format | Online Article Text |
id | pubmed-6330284 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63302842019-01-21 Time- and Ventricular-Specific Expression Profiles of Genes Encoding Z-Disk Proteins in Pressure Overload Model of Left Ventricular Hypertrophy Knyazeva, Anastasia Krutikov, Alexander Golovkin, Alexey Mishanin, Alexander Pavlov, Georgii Smolina, Natalia Hushkina, Anastasia Sejersen, Thomas Sjoberg, Gunnar Galagudza, Mikhail Kostareva, Anna Front Genet Genetics Mechanotransduction is an essential mechanism of transforming external mechanical stimulus to biochemical response. In cardiomyocytes mechanotransduction plays an important role in contraction, stretch sensing and homeostasis regulation. One of the major mechanosensitive area in cardiomyocytes, the Z-disk, consists of numbers of structural and signaling proteins, that may undergo conformational or gene expression changes under pathological stress conditions. In present study we examined a rat model of pressure overload cardiac hypertrophy validated by echocardiographic and histopathological examinations. We revealed, that during hypertrophy progression expression of several genes encoding Z-disk proteins (Actn2, Ldb3, Cmya5, Nebl) is different at early and late points of cardiac remodeling. Moreover, expression patterns of several genes are opposite in myocardium of overloaded left ventricle and hemodynamically unaffected right ventricle, and expression profiles in interventricular septum are more similar to right ventricle. Additionally, we revealed inconsistencies between mRNA and protein level changes of Actn2, one of the major structural Z-disk element. All these findings point out, that investigated Z-disk proteins participate in pathological stress adaptation through undergoing the gene expression changes, and suggest the novel important role of hypertrophic response modulation during different stages of cardiac remodeling. Frontiers Media S.A. 2019-01-07 /pmc/articles/PMC6330284/ /pubmed/30666270 http://dx.doi.org/10.3389/fgene.2018.00684 Text en Copyright © 2019 Knyazeva, Krutikov, Golovkin, Mishanin, Pavlov, Smolina, Hushkina, Sejersen, Sjoberg, Galagudza and Kostareva. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Knyazeva, Anastasia Krutikov, Alexander Golovkin, Alexey Mishanin, Alexander Pavlov, Georgii Smolina, Natalia Hushkina, Anastasia Sejersen, Thomas Sjoberg, Gunnar Galagudza, Mikhail Kostareva, Anna Time- and Ventricular-Specific Expression Profiles of Genes Encoding Z-Disk Proteins in Pressure Overload Model of Left Ventricular Hypertrophy |
title | Time- and Ventricular-Specific Expression Profiles of Genes Encoding Z-Disk Proteins in Pressure Overload Model of Left Ventricular Hypertrophy |
title_full | Time- and Ventricular-Specific Expression Profiles of Genes Encoding Z-Disk Proteins in Pressure Overload Model of Left Ventricular Hypertrophy |
title_fullStr | Time- and Ventricular-Specific Expression Profiles of Genes Encoding Z-Disk Proteins in Pressure Overload Model of Left Ventricular Hypertrophy |
title_full_unstemmed | Time- and Ventricular-Specific Expression Profiles of Genes Encoding Z-Disk Proteins in Pressure Overload Model of Left Ventricular Hypertrophy |
title_short | Time- and Ventricular-Specific Expression Profiles of Genes Encoding Z-Disk Proteins in Pressure Overload Model of Left Ventricular Hypertrophy |
title_sort | time- and ventricular-specific expression profiles of genes encoding z-disk proteins in pressure overload model of left ventricular hypertrophy |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330284/ https://www.ncbi.nlm.nih.gov/pubmed/30666270 http://dx.doi.org/10.3389/fgene.2018.00684 |
work_keys_str_mv | AT knyazevaanastasia timeandventricularspecificexpressionprofilesofgenesencodingzdiskproteinsinpressureoverloadmodelofleftventricularhypertrophy AT krutikovalexander timeandventricularspecificexpressionprofilesofgenesencodingzdiskproteinsinpressureoverloadmodelofleftventricularhypertrophy AT golovkinalexey timeandventricularspecificexpressionprofilesofgenesencodingzdiskproteinsinpressureoverloadmodelofleftventricularhypertrophy AT mishaninalexander timeandventricularspecificexpressionprofilesofgenesencodingzdiskproteinsinpressureoverloadmodelofleftventricularhypertrophy AT pavlovgeorgii timeandventricularspecificexpressionprofilesofgenesencodingzdiskproteinsinpressureoverloadmodelofleftventricularhypertrophy AT smolinanatalia timeandventricularspecificexpressionprofilesofgenesencodingzdiskproteinsinpressureoverloadmodelofleftventricularhypertrophy AT hushkinaanastasia timeandventricularspecificexpressionprofilesofgenesencodingzdiskproteinsinpressureoverloadmodelofleftventricularhypertrophy AT sejersenthomas timeandventricularspecificexpressionprofilesofgenesencodingzdiskproteinsinpressureoverloadmodelofleftventricularhypertrophy AT sjoberggunnar timeandventricularspecificexpressionprofilesofgenesencodingzdiskproteinsinpressureoverloadmodelofleftventricularhypertrophy AT galagudzamikhail timeandventricularspecificexpressionprofilesofgenesencodingzdiskproteinsinpressureoverloadmodelofleftventricularhypertrophy AT kostarevaanna timeandventricularspecificexpressionprofilesofgenesencodingzdiskproteinsinpressureoverloadmodelofleftventricularhypertrophy |