Cargando…
Juglans Regia L. Leaf Extract Attenuates Diabetic Nephropathy Progression in Experimental Diabetes: An Immunohistochemical Study
BACKGROUND: There is accumulating evidence that Juglans regia L. (GRL) leaf extract has hypoglycemic and antioxidative properties. The present study aimed to investigate the protective effects of GRL leaf extract against diabetic nephropathy (DN). METHODS: In total, 28 male adult Sprague-Dawley rats...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Iranian Journal of Medical Sciences
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330519/ https://www.ncbi.nlm.nih.gov/pubmed/30666075 |
_version_ | 1783386992723099648 |
---|---|
author | Nasiry, Davood Khalatbary, Ali Reza Ahmadvand, Hassan Talebpour Amiri, Fereshteh |
author_facet | Nasiry, Davood Khalatbary, Ali Reza Ahmadvand, Hassan Talebpour Amiri, Fereshteh |
author_sort | Nasiry, Davood |
collection | PubMed |
description | BACKGROUND: There is accumulating evidence that Juglans regia L. (GRL) leaf extract has hypoglycemic and antioxidative properties. The present study aimed to investigate the protective effects of GRL leaf extract against diabetic nephropathy (DN). METHODS: In total, 28 male adult Sprague-Dawley rats were used. The DN rat model was generated by intraperitoneal injection of a single 55 mg/kg dose of streptozotocin (STZ). A subset of the STZ-induced diabetic rats received intragastric administration of GRL leaf extract (200 mg/kg/day) starting 1 week (preventive group) and 4 weeks (curative group) after the onset of hyperglycemia up to the end of the 8th week, whereas other diabetic rats received only isotonic saline (diabetic group) as the same volume of GRL leaf extract. To evaluate the effects of GRL leaf extract on the diabetic nephropathy, various parameters of apoptosis and inflammation were assessed. Statistical analysis was performed using the SPSS software, version 15.0. The data were compared between the groups using the Tukey’s multiple comparison test and the analysis of the variance. P values ˂0.05 were considered statistically significant. RESULTS: Fasting blood sugar (FBS) levels (P=0.001) and histopathological changes in the kidney of diabetic rats attenuated after GRL leaf extract consumption. Greater caspase-3 (P=0.004), COX-2 (P=0.008), PARP (P=0.007), and iNOS (P=0.005) expression could be detected in the STZ-diabetic rats, which were significantly (P=0.009) attenuated after GRL leaf extract consumption. In addition, attenuation of lipid peroxidation in the diabetic rats was detected after GRL consumption (P=0.01). CONCLUSION: GRL leaf extract exerts preventive and curative effects against diabetic nephropathy. |
format | Online Article Text |
id | pubmed-6330519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Iranian Journal of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-63305192019-01-21 Juglans Regia L. Leaf Extract Attenuates Diabetic Nephropathy Progression in Experimental Diabetes: An Immunohistochemical Study Nasiry, Davood Khalatbary, Ali Reza Ahmadvand, Hassan Talebpour Amiri, Fereshteh Iran J Med Sci Original Article BACKGROUND: There is accumulating evidence that Juglans regia L. (GRL) leaf extract has hypoglycemic and antioxidative properties. The present study aimed to investigate the protective effects of GRL leaf extract against diabetic nephropathy (DN). METHODS: In total, 28 male adult Sprague-Dawley rats were used. The DN rat model was generated by intraperitoneal injection of a single 55 mg/kg dose of streptozotocin (STZ). A subset of the STZ-induced diabetic rats received intragastric administration of GRL leaf extract (200 mg/kg/day) starting 1 week (preventive group) and 4 weeks (curative group) after the onset of hyperglycemia up to the end of the 8th week, whereas other diabetic rats received only isotonic saline (diabetic group) as the same volume of GRL leaf extract. To evaluate the effects of GRL leaf extract on the diabetic nephropathy, various parameters of apoptosis and inflammation were assessed. Statistical analysis was performed using the SPSS software, version 15.0. The data were compared between the groups using the Tukey’s multiple comparison test and the analysis of the variance. P values ˂0.05 were considered statistically significant. RESULTS: Fasting blood sugar (FBS) levels (P=0.001) and histopathological changes in the kidney of diabetic rats attenuated after GRL leaf extract consumption. Greater caspase-3 (P=0.004), COX-2 (P=0.008), PARP (P=0.007), and iNOS (P=0.005) expression could be detected in the STZ-diabetic rats, which were significantly (P=0.009) attenuated after GRL leaf extract consumption. In addition, attenuation of lipid peroxidation in the diabetic rats was detected after GRL consumption (P=0.01). CONCLUSION: GRL leaf extract exerts preventive and curative effects against diabetic nephropathy. Iranian Journal of Medical Sciences 2019-01 /pmc/articles/PMC6330519/ /pubmed/30666075 Text en Copyright: © Iranian Journal of Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Nasiry, Davood Khalatbary, Ali Reza Ahmadvand, Hassan Talebpour Amiri, Fereshteh Juglans Regia L. Leaf Extract Attenuates Diabetic Nephropathy Progression in Experimental Diabetes: An Immunohistochemical Study |
title | Juglans Regia L. Leaf Extract Attenuates Diabetic Nephropathy Progression in Experimental Diabetes: An Immunohistochemical Study
|
title_full | Juglans Regia L. Leaf Extract Attenuates Diabetic Nephropathy Progression in Experimental Diabetes: An Immunohistochemical Study
|
title_fullStr | Juglans Regia L. Leaf Extract Attenuates Diabetic Nephropathy Progression in Experimental Diabetes: An Immunohistochemical Study
|
title_full_unstemmed | Juglans Regia L. Leaf Extract Attenuates Diabetic Nephropathy Progression in Experimental Diabetes: An Immunohistochemical Study
|
title_short | Juglans Regia L. Leaf Extract Attenuates Diabetic Nephropathy Progression in Experimental Diabetes: An Immunohistochemical Study
|
title_sort | juglans regia l. leaf extract attenuates diabetic nephropathy progression in experimental diabetes: an immunohistochemical study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330519/ https://www.ncbi.nlm.nih.gov/pubmed/30666075 |
work_keys_str_mv | AT nasirydavood juglansregialleafextractattenuatesdiabeticnephropathyprogressioninexperimentaldiabetesanimmunohistochemicalstudy AT khalatbaryalireza juglansregialleafextractattenuatesdiabeticnephropathyprogressioninexperimentaldiabetesanimmunohistochemicalstudy AT ahmadvandhassan juglansregialleafextractattenuatesdiabeticnephropathyprogressioninexperimentaldiabetesanimmunohistochemicalstudy AT talebpouramirifereshteh juglansregialleafextractattenuatesdiabeticnephropathyprogressioninexperimentaldiabetesanimmunohistochemicalstudy |