Using CRISPR/Cas9 genome editing in tomato to create a gibberellin‐responsive dominant dwarf DELLA allele

The tomato PROCERA gene encodes a DELLA protein, and loss‐of‐function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss‐of‐function mutations and a dominant dwarf mutation that carries a deletio...

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Detalles Bibliográficos
Autores principales: Tomlinson, Laurence, Yang, Ying, Emenecker, Ryan, Smoker, Matthew, Taylor, Jodie, Perkins, Sara, Smith, Justine, MacLean, Dan, Olszewski, Neil E., Jones, Jonathan D. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330640/
https://www.ncbi.nlm.nih.gov/pubmed/29797460
http://dx.doi.org/10.1111/pbi.12952

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