Cysteine Disulfides (Cys-ss-X) as Sensitive Plasma Biomarkers of Oxidative Stress

We developed a high-throughput mass spectrometry–based method to simultaneously quantify numerous small-molecule thiols and disulfides in blood plasma. Application of this assay to analyze plasma from patients with known oxidative stress (sickle cell disease and sepsis) and from a patient with sickl...

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Autores principales: Fu, Xiaoyun, Cate, Shelby A., Dominguez, Melissa, Osborn, Warren, Özpolat, Tahsin, Konkle, Barbara A., Chen, Junmei, López, José A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331564/
https://www.ncbi.nlm.nih.gov/pubmed/30643157
http://dx.doi.org/10.1038/s41598-018-35566-2
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author Fu, Xiaoyun
Cate, Shelby A.
Dominguez, Melissa
Osborn, Warren
Özpolat, Tahsin
Konkle, Barbara A.
Chen, Junmei
López, José A.
author_facet Fu, Xiaoyun
Cate, Shelby A.
Dominguez, Melissa
Osborn, Warren
Özpolat, Tahsin
Konkle, Barbara A.
Chen, Junmei
López, José A.
author_sort Fu, Xiaoyun
collection PubMed
description We developed a high-throughput mass spectrometry–based method to simultaneously quantify numerous small-molecule thiols and disulfides in blood plasma. Application of this assay to analyze plasma from patients with known oxidative stress (sickle cell disease and sepsis) and from a patient with sickle cell disease treated with the antioxidant N-acetylcysteine suggests that cysteine disulfides, in particular protein-bound cysteine, serve as sensitive plasma biomarkers for the extent of oxidative stress and effectiveness of antioxidant treatment.
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spelling pubmed-63315642019-01-16 Cysteine Disulfides (Cys-ss-X) as Sensitive Plasma Biomarkers of Oxidative Stress Fu, Xiaoyun Cate, Shelby A. Dominguez, Melissa Osborn, Warren Özpolat, Tahsin Konkle, Barbara A. Chen, Junmei López, José A. Sci Rep Article We developed a high-throughput mass spectrometry–based method to simultaneously quantify numerous small-molecule thiols and disulfides in blood plasma. Application of this assay to analyze plasma from patients with known oxidative stress (sickle cell disease and sepsis) and from a patient with sickle cell disease treated with the antioxidant N-acetylcysteine suggests that cysteine disulfides, in particular protein-bound cysteine, serve as sensitive plasma biomarkers for the extent of oxidative stress and effectiveness of antioxidant treatment. Nature Publishing Group UK 2019-01-14 /pmc/articles/PMC6331564/ /pubmed/30643157 http://dx.doi.org/10.1038/s41598-018-35566-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Fu, Xiaoyun
Cate, Shelby A.
Dominguez, Melissa
Osborn, Warren
Özpolat, Tahsin
Konkle, Barbara A.
Chen, Junmei
López, José A.
Cysteine Disulfides (Cys-ss-X) as Sensitive Plasma Biomarkers of Oxidative Stress
title Cysteine Disulfides (Cys-ss-X) as Sensitive Plasma Biomarkers of Oxidative Stress
title_full Cysteine Disulfides (Cys-ss-X) as Sensitive Plasma Biomarkers of Oxidative Stress
title_fullStr Cysteine Disulfides (Cys-ss-X) as Sensitive Plasma Biomarkers of Oxidative Stress
title_full_unstemmed Cysteine Disulfides (Cys-ss-X) as Sensitive Plasma Biomarkers of Oxidative Stress
title_short Cysteine Disulfides (Cys-ss-X) as Sensitive Plasma Biomarkers of Oxidative Stress
title_sort cysteine disulfides (cys-ss-x) as sensitive plasma biomarkers of oxidative stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331564/
https://www.ncbi.nlm.nih.gov/pubmed/30643157
http://dx.doi.org/10.1038/s41598-018-35566-2
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