Cargando…

LDH-A promotes malignant behavior via activation of epithelial-to-mesenchymal transition in lung adenocarcinoma

Lactate dehydrogenase A (LDH-A) is a key enzyme during glycolysis, which increases the synthesis of related proteins and has elevated activity in cancer cells. The role of LDH-A in lung adenocarcinoma (LUAD) progression was investigated in the present study. Expression levels of LDH-A were assessed...

Descripción completa

Detalles Bibliográficos
Autores principales: Hou, Xiao-ming, Yuan, Shu-qiao, Zhao, Da, Liu, Xiao-jun, Wu, Xin-an
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331674/
https://www.ncbi.nlm.nih.gov/pubmed/30509961
http://dx.doi.org/10.1042/BSR20181476
_version_ 1783387180537741312
author Hou, Xiao-ming
Yuan, Shu-qiao
Zhao, Da
Liu, Xiao-jun
Wu, Xin-an
author_facet Hou, Xiao-ming
Yuan, Shu-qiao
Zhao, Da
Liu, Xiao-jun
Wu, Xin-an
author_sort Hou, Xiao-ming
collection PubMed
description Lactate dehydrogenase A (LDH-A) is a key enzyme during glycolysis, which increases the synthesis of related proteins and has elevated activity in cancer cells. The role of LDH-A in lung adenocarcinoma (LUAD) progression was investigated in the present study. Expression levels of LDH-A were assessed in LUAD samples, and the relationship between LDH-A expression status and the prognosis of LUAD patients was confirmed. The effect of LDH-A on proliferation, invasion, migration, and colony formation of cancer cells was assessed. We further determined the role of LDH-A in tumor growth in vivo by using xenograft LUAD tumor models. The potential mechanism of LDH-A promotion in LUAD progression was explored. LDH-A showed an abnormally high expression in LUAD, which is closely associated with poor prognosis in patients with LUAD. In in vitro experiments, silencing LDH-A expression in LUAD cells could effectively inhibit proliferation, invasion, migration, and colony formation of cancer cells. In in vivo experiments, tumor growth was markedly inhibited by LDH-A silencing in a xenograft model of LUAD. Notably, LDH-A could also promote tumor progression by regulating epithelial–mesenchymal transition (EMT)-related molecules. LDH-A can promote the malignant biological behaviors of LUAD cells, and thus can be a potential target for LUAD treatment.
format Online
Article
Text
id pubmed-6331674
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-63316742019-01-23 LDH-A promotes malignant behavior via activation of epithelial-to-mesenchymal transition in lung adenocarcinoma Hou, Xiao-ming Yuan, Shu-qiao Zhao, Da Liu, Xiao-jun Wu, Xin-an Biosci Rep Research Articles Lactate dehydrogenase A (LDH-A) is a key enzyme during glycolysis, which increases the synthesis of related proteins and has elevated activity in cancer cells. The role of LDH-A in lung adenocarcinoma (LUAD) progression was investigated in the present study. Expression levels of LDH-A were assessed in LUAD samples, and the relationship between LDH-A expression status and the prognosis of LUAD patients was confirmed. The effect of LDH-A on proliferation, invasion, migration, and colony formation of cancer cells was assessed. We further determined the role of LDH-A in tumor growth in vivo by using xenograft LUAD tumor models. The potential mechanism of LDH-A promotion in LUAD progression was explored. LDH-A showed an abnormally high expression in LUAD, which is closely associated with poor prognosis in patients with LUAD. In in vitro experiments, silencing LDH-A expression in LUAD cells could effectively inhibit proliferation, invasion, migration, and colony formation of cancer cells. In in vivo experiments, tumor growth was markedly inhibited by LDH-A silencing in a xenograft model of LUAD. Notably, LDH-A could also promote tumor progression by regulating epithelial–mesenchymal transition (EMT)-related molecules. LDH-A can promote the malignant biological behaviors of LUAD cells, and thus can be a potential target for LUAD treatment. Portland Press Ltd. 2019-01-15 /pmc/articles/PMC6331674/ /pubmed/30509961 http://dx.doi.org/10.1042/BSR20181476 Text en © 2019 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Hou, Xiao-ming
Yuan, Shu-qiao
Zhao, Da
Liu, Xiao-jun
Wu, Xin-an
LDH-A promotes malignant behavior via activation of epithelial-to-mesenchymal transition in lung adenocarcinoma
title LDH-A promotes malignant behavior via activation of epithelial-to-mesenchymal transition in lung adenocarcinoma
title_full LDH-A promotes malignant behavior via activation of epithelial-to-mesenchymal transition in lung adenocarcinoma
title_fullStr LDH-A promotes malignant behavior via activation of epithelial-to-mesenchymal transition in lung adenocarcinoma
title_full_unstemmed LDH-A promotes malignant behavior via activation of epithelial-to-mesenchymal transition in lung adenocarcinoma
title_short LDH-A promotes malignant behavior via activation of epithelial-to-mesenchymal transition in lung adenocarcinoma
title_sort ldh-a promotes malignant behavior via activation of epithelial-to-mesenchymal transition in lung adenocarcinoma
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331674/
https://www.ncbi.nlm.nih.gov/pubmed/30509961
http://dx.doi.org/10.1042/BSR20181476
work_keys_str_mv AT houxiaoming ldhapromotesmalignantbehaviorviaactivationofepithelialtomesenchymaltransitioninlungadenocarcinoma
AT yuanshuqiao ldhapromotesmalignantbehaviorviaactivationofepithelialtomesenchymaltransitioninlungadenocarcinoma
AT zhaoda ldhapromotesmalignantbehaviorviaactivationofepithelialtomesenchymaltransitioninlungadenocarcinoma
AT liuxiaojun ldhapromotesmalignantbehaviorviaactivationofepithelialtomesenchymaltransitioninlungadenocarcinoma
AT wuxinan ldhapromotesmalignantbehaviorviaactivationofepithelialtomesenchymaltransitioninlungadenocarcinoma