Distribution of tumor-infiltrating immune cells in glioblastoma

AIM: Evaluation of features related to infiltrating immune cell level in glioblastoma. METHODS: Tumor-infiltrating lymphocytes (TILs) through H&E staining, and TILs (CD3, CD4, CD8 and CD20) and macrophage (CD68 and CD163) levels through immunohistochemistry were evaluated through digital analysi...

Descripción completa

Detalles Bibliográficos
Autores principales: Orrego, Enrique, Castaneda, Carlos A, Castillo, Miluska, Bernabe, Luis A, Casavilca, Sandro, Chakravarti, Arnab, Meng, Wei, Garcia-Corrochano, Pamela, Villa-Robles, Maria R, Zevallos, Rocio, Mejia, Omar, Deza, Pedro, Belmar-Lopez, Carolina, Ojeda, Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Medicine Ltd 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331699/
https://www.ncbi.nlm.nih.gov/pubmed/30299157
http://dx.doi.org/10.2217/cns-2017-0037
Descripción
Sumario:AIM: Evaluation of features related to infiltrating immune cell level in glioblastoma. METHODS: Tumor-infiltrating lymphocytes (TILs) through H&E staining, and TILs (CD3, CD4, CD8 and CD20) and macrophage (CD68 and CD163) levels through immunohistochemistry were evaluated through digital analysis. RESULTS: CD68 (9.1%), CD163 (2.2%), CD3 (1.6%) and CD8 (1.6%) had the highest density. Higher CD4(+) was associated with unmethylated MGMT (p = 0.016). Higher CD8(+) was associated with larger tumoral size (p = 0.027). Higher CD163(+) was associated with higher age (p = 0.044) and recursive partitioning analysis = 4. Women (p < 0.05), total resection (p < 0.05), MGMT-methylation (p < 0.001), radiotherapy (p < 0.001), chemotherapy (p < 0.001) and lower CD4(+) (p < 0.05) were associated with longer overall survival. CONCLUSION: Macrophages are more frequent than TILs. Some subsets are associated with clinical features.

Ejemplares similares