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Ultracentrifugation versus kit exosome isolation: nanoLC–MS and other tools reveal similar performance biomarkers, but also contaminations
AIM: For isolation of exosomes, differential ultracentrifugation and an isolation kit from a major vendor were compared. MATERIALS & METHODS: ‘Case study’ exosomes isolated from patient-derived cells from glioblastoma multiforme and a breast cancer cell line were analyzed. RESULTS: Transmission...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Future Science Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331754/ https://www.ncbi.nlm.nih.gov/pubmed/30652024 http://dx.doi.org/10.4155/fsoa-2018-0088 |
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author | Skottvoll, Frøydis Sved Berg, Henriette Engen Bjørseth, Kamilla Lund, Kaja Roos, Norbert Bekhradnia, Sara Thiede, Bernd Sandberg, Cecilie Vik-Mo, Einar Osland Roberg-Larsen, Hanne Nyström, Bo Lundanes, Elsa Wilson, Steven Ray |
author_facet | Skottvoll, Frøydis Sved Berg, Henriette Engen Bjørseth, Kamilla Lund, Kaja Roos, Norbert Bekhradnia, Sara Thiede, Bernd Sandberg, Cecilie Vik-Mo, Einar Osland Roberg-Larsen, Hanne Nyström, Bo Lundanes, Elsa Wilson, Steven Ray |
author_sort | Skottvoll, Frøydis Sved |
collection | PubMed |
description | AIM: For isolation of exosomes, differential ultracentrifugation and an isolation kit from a major vendor were compared. MATERIALS & METHODS: ‘Case study’ exosomes isolated from patient-derived cells from glioblastoma multiforme and a breast cancer cell line were analyzed. RESULTS: Transmission electron microscopy, dynamic light scattering, western blotting, and so forth, revealed comparable performance. Potential protein biomarkers for both diseases were also identified in the isolates using nanoLC–MS. Western blotting and nanoLC–MS also revealed negative exosome markers regarding both isolation approaches. CONCLUSION: The two isolation methods had an overall similar performance, but we hesitate to use the term ‘exosome isolation’ as impurities may be present with both isolation methods. NanoLC–MS can detect disease biomarkers in exosomes and is useful for critical assessment of exosome enrichment procedures. |
format | Online Article Text |
id | pubmed-6331754 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Future Science Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-63317542019-01-16 Ultracentrifugation versus kit exosome isolation: nanoLC–MS and other tools reveal similar performance biomarkers, but also contaminations Skottvoll, Frøydis Sved Berg, Henriette Engen Bjørseth, Kamilla Lund, Kaja Roos, Norbert Bekhradnia, Sara Thiede, Bernd Sandberg, Cecilie Vik-Mo, Einar Osland Roberg-Larsen, Hanne Nyström, Bo Lundanes, Elsa Wilson, Steven Ray Future Sci OA Research Article AIM: For isolation of exosomes, differential ultracentrifugation and an isolation kit from a major vendor were compared. MATERIALS & METHODS: ‘Case study’ exosomes isolated from patient-derived cells from glioblastoma multiforme and a breast cancer cell line were analyzed. RESULTS: Transmission electron microscopy, dynamic light scattering, western blotting, and so forth, revealed comparable performance. Potential protein biomarkers for both diseases were also identified in the isolates using nanoLC–MS. Western blotting and nanoLC–MS also revealed negative exosome markers regarding both isolation approaches. CONCLUSION: The two isolation methods had an overall similar performance, but we hesitate to use the term ‘exosome isolation’ as impurities may be present with both isolation methods. NanoLC–MS can detect disease biomarkers in exosomes and is useful for critical assessment of exosome enrichment procedures. Future Science Ltd 2018-11-09 /pmc/articles/PMC6331754/ /pubmed/30652024 http://dx.doi.org/10.4155/fsoa-2018-0088 Text en © 2018 The Authors This work is licensed under a Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/) |
spellingShingle | Research Article Skottvoll, Frøydis Sved Berg, Henriette Engen Bjørseth, Kamilla Lund, Kaja Roos, Norbert Bekhradnia, Sara Thiede, Bernd Sandberg, Cecilie Vik-Mo, Einar Osland Roberg-Larsen, Hanne Nyström, Bo Lundanes, Elsa Wilson, Steven Ray Ultracentrifugation versus kit exosome isolation: nanoLC–MS and other tools reveal similar performance biomarkers, but also contaminations |
title | Ultracentrifugation versus kit exosome isolation: nanoLC–MS and other tools reveal similar performance biomarkers, but also contaminations |
title_full | Ultracentrifugation versus kit exosome isolation: nanoLC–MS and other tools reveal similar performance biomarkers, but also contaminations |
title_fullStr | Ultracentrifugation versus kit exosome isolation: nanoLC–MS and other tools reveal similar performance biomarkers, but also contaminations |
title_full_unstemmed | Ultracentrifugation versus kit exosome isolation: nanoLC–MS and other tools reveal similar performance biomarkers, but also contaminations |
title_short | Ultracentrifugation versus kit exosome isolation: nanoLC–MS and other tools reveal similar performance biomarkers, but also contaminations |
title_sort | ultracentrifugation versus kit exosome isolation: nanolc–ms and other tools reveal similar performance biomarkers, but also contaminations |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331754/ https://www.ncbi.nlm.nih.gov/pubmed/30652024 http://dx.doi.org/10.4155/fsoa-2018-0088 |
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