Ultracentrifugation versus kit exosome isolation: nanoLC–MS and other tools reveal similar performance biomarkers, but also contaminations

AIM: For isolation of exosomes, differential ultracentrifugation and an isolation kit from a major vendor were compared. MATERIALS & METHODS: ‘Case study’ exosomes isolated from patient-derived cells from glioblastoma multiforme and a breast cancer cell line were analyzed. RESULTS: Transmission...

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Detalles Bibliográficos
Autores principales: Skottvoll, Frøydis Sved, Berg, Henriette Engen, Bjørseth, Kamilla, Lund, Kaja, Roos, Norbert, Bekhradnia, Sara, Thiede, Bernd, Sandberg, Cecilie, Vik-Mo, Einar Osland, Roberg-Larsen, Hanne, Nyström, Bo, Lundanes, Elsa, Wilson, Steven Ray
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331754/
https://www.ncbi.nlm.nih.gov/pubmed/30652024
http://dx.doi.org/10.4155/fsoa-2018-0088

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