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Optimization of a peptide extraction and LC–MS protocol for quantitative analysis of antimicrobial peptides

We optimized a peptide extraction and LC–MS protocol for identification and quantification of antimicrobial peptides (AMPs) in biological samples. Amphipathic AMPs were extracted with various concentrations of ethanol, methanol, acetonitrile, formic acid, acetic acid or trichloroacetic acid in water...

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Detalles Bibliográficos
Autores principales: Chen, Wen, Hwang, Yoon Y, Gleaton, Jeremy W, Titus, James K, Hamlin, Nicholas J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331757/
https://www.ncbi.nlm.nih.gov/pubmed/30652017
http://dx.doi.org/10.4155/fsoa-2018-0073