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Optimization of a peptide extraction and LC–MS protocol for quantitative analysis of antimicrobial peptides
We optimized a peptide extraction and LC–MS protocol for identification and quantification of antimicrobial peptides (AMPs) in biological samples. Amphipathic AMPs were extracted with various concentrations of ethanol, methanol, acetonitrile, formic acid, acetic acid or trichloroacetic acid in water...
Autores principales: | Chen, Wen, Hwang, Yoon Y, Gleaton, Jeremy W, Titus, James K, Hamlin, Nicholas J |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Future Science Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331757/ https://www.ncbi.nlm.nih.gov/pubmed/30652017 http://dx.doi.org/10.4155/fsoa-2018-0073 |
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